2-[6-[2-[9-(2-Aminoethyl)purin-8-yl]ethynyl]purin-9-yl]ethanamine | 1426313-61-2

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 咪唑并嘧啶类
• 英文名称: 2-[6-[2-[9-(2-Aminoethyl)purin-8-yl]ethynyl]purin-9-yl]ethanamine
• 英文别名: 2-[6-[2-[9-(2-aminoethyl)purin-8-yl]ethynyl]purin-9-yl]ethanamine
• CAS: 1426313-61-2
• 化学式: C₁₆H₁₆N₁₀
• 分子量: 348.37
• InChiKey: SPIYAWDSYNHMOG-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -1.7
• 重原子数：
  26
• 可旋转键数：
  6
• 环数：
  4.0
• sp3杂化的碳原子比例：
  0.25
• 拓扑面积：
  139
• 氢给体数：
  2
• 氢受体数：
  8

反应信息

• 作为产物：
  描述：

  2-(adenin-9-yl)-N-<(tert-butyloxy)carbonyl>ethylamine 在 四氢呋喃 、 copper(l) iodide 、 四(三苯基膦)钯 、 亚硝酸正戊酯 、 四丁基氟化铵 、 溴 、 sodium acetate 、 四氯化锡 、 caesium carbonate 作用下， 以 四氢呋喃 、 甲醇 、 水 、 乙酸乙酯 为溶剂， 反应 13.5h， 生成 2-[6-[2-[9-(2-Aminoethyl)purin-8-yl]ethynyl]purin-9-yl]ethanamine
  参考文献：
  名称：

  乙炔桥 6,8-嘌呤二聚体作为平行 G-四链体的荧光开关探针

  摘要：

  点亮：报道了由两个乙炔桥嘌呤组成的 G 四联体荧光探针（APD）的系统设计和合成。APD 在平行 DNA（例如 c-MYC）或 RNA G-四链体存在时亮起，而双链 DNA、反平行或混合型（例如 h-Telo）G-四链体则没有荧光增强（参见图片）。还证明了 APD 在 G-四链体优先染色中的实用性。

  DOI：

  10.1002/anie.201207075

文献信息

• Method for detecting target molecule in which rolling circle amplification is used
  申请人：National University Corporation Gunma University

  公开号：US11359240B2

  公开（公告）日：2022-06-14

  A method of detecting a target molecule, the method comprising: forming a complex of a target molecule, a capture oligonucleotide, an oligonucleotide primer, and a single-stranded circular DNA; performing a nucleic acid amplification reaction by rolling circle amplification based on the formation of the complex; and detecting amplified nucleic acid; wherein the single-stranded circular DNA contains a first region, and a second region linked to the 3′-side of the first region, and preferably further contains a sequence complementary to a detection reagent-binding sequence; the primer contains a first aptamer sequence which binds to the target molecule, and a sequence which is linked to the 3′-side of the first aptamer sequence and is complementary to the first region of the single-stranded circular DNA; and the capture oligonucleotide contains a sequence complementary to the second region of the single-stranded circular DNA, and a second aptamer sequence which is linked to the 3′-side of the sequence complementary to the second region and binds to the target molecule.

  一种检测目标分子的方法，该方法包括形成目标分子、捕获寡核苷酸、寡核苷酸引物和单链环状 DNA 的复合物；根据复合物的形成，通过滚圆扩增进行核酸扩增反应；以及检测扩增的核酸；其中，单链环状 DNA 包含第一区域和与第一区域的 3′-侧相连的第二区域，并且最好进一步包含与检测试剂结合序列互补的序列；引物包含与目标分子结合的第一适配体序列，以及与第一适配体序列的 3′-侧连接并与单链环状 DNA 的第一区域互补的序列；捕获寡核苷酸包含与单链环状 DNA 第二区域互补的序列，以及与第二区域互补序列的 3′-侧相连并与靶分子结合的第二适配体序列。
• Simple Method for Detecting RNA Sequences
  申请人：National University Corporation Gunma University

  公开号：US20190040465A1

  公开（公告）日：2019-02-07

  An RNA detection kit comprising: (i) a single-stranded circular DNA template containing: a sequence of 10 to 30 bases complementary to a first portion of a target RNA; a primer-binding sequence of 7 to 8 bases adjacent to 5′-side thereof; and a sequence complementary to a detection reagent-binding sequence such as a guanine quadruplex-forming sequence; (ii) an oligonucleotide primer containing: a sequence of 8 to 15 bases complementary to a second portion adjacent to the 3′-side of the first portion of the target RNA; and a sequence of 7 to 8 bases adjacent to 3′-side thereof and complementary to the primer-binding sequence of the single-stranded circular DNA template; and (iii) a detection reagent such as a guanine quadruplex-binding reagent; is provided.
• METHOD FOR DETECTING TARGET MOLECULE IN WHICH ROLLING CIRCLE AMPLIFICATION IS USED
  申请人：National University Corporation Gunma University

  公开号：US20200071759A1

  公开（公告）日：2020-03-05

  A method of detecting a target molecule, the method comprising: forming a complex of a target molecule, a capture oligonucleotide, an oligonucleotide primer, and a single-stranded circular DNA; performing a nucleic acid amplification reaction by rolling circle amplification based on the formation of the complex; and detecting amplified nucleic acid; wherein the single-stranded circular DNA contains a first region, and a second region linked to the 3′-side of the first region, and preferably further contains a sequence complementary to a detection reagent-binding sequence; the primer contains a first aptamer sequence which binds to the target molecule, and a sequence which is linked to the 3′-side of the first aptamer sequence and is complementary to the first region of the single-stranded circular DNA; and the capture oligonucleotide contains a sequence complementary to the second region of the single-stranded circular DNA, and a second aptamer sequence which is linked to the 3′-side of the sequence complementary to the second region and binds to the target molecule.
• SIMPLE METHOD FOR DETECTING NUCLEIC ACID SEQUENCE, ETC.
  申请人：Nihon University

  公开号：US20220213525A1

  公开（公告）日：2022-07-07

  A nucleic acid detection kit including: (i) a first single-stranded circular DNA; (ii) a first oligonucleotide primer; (iii) a second single-stranded circular DNA; and (iv) a second oligonucleotide primer; wherein the first oligonucleotide primer is hound to a carrier through the 5′-end thereof, and the second oligonucleotide primer is bound, through the 5′-end thereof, to the carrier to which the first oligonucleotide primer is hound.
• An Acetylene-Bridged 6,8-Purine Dimer as a Fluorescent Switch-On Probe for Parallel G-Quadruplexes
  作者：Mehran Nikan、Marco Di Antonio、Keren Abecassis、Keith McLuckie、Shankar Balasubramanian

  DOI：10.1002/anie.201207075

  日期：2013.1.28

  Lighting up: The systematic design and synthesis of a G‐quartet‐inspired fluorescence probe (APD), which is made of two acetylene‐bridged purines, are reported. The APD lights up in the presence of parallel DNA (e.g. c‐MYC) or RNA G‐quadruplexes, while it shows no fluorescence enhancement with double‐stranded DNA, antiparallel or mixed‐type (e.g. h‐Telo) G‐quadruplexes (see picture). The utility of

  点亮：报道了由两个乙炔桥嘌呤组成的 G 四联体荧光探针（APD）的系统设计和合成。APD 在平行 DNA（例如 c-MYC）或 RNA G-四链体存在时亮起，而双链 DNA、反平行或混合型（例如 h-Telo）G-四链体则没有荧光增强（参见图片）。还证明了 APD 在 G-四链体优先染色中的实用性。