申请人:National Institute of Advanced Industrial Science and Technology
公开号:EP1365017A1
公开(公告)日:2003-11-26
The present invention provides a photo-responsive composition comprising a photo-responsive material having the property of differential adhesiveness in response to changes in light irradiation. The photo-responsive composition is used as an adhesive surface for the growth anchorage-dependent cells in a culturing dish. The position of a particular cell on the photo-responsive composition is irradiated with light to release only the cell attached at that position, and the released cell may be sortingly collected. According to the present invention, cells including anchorage-dependent cells can be sortingly collected through a simple operation while maintaining the extracellular matrix and membrane proteins of the cells, as well as the organ-specific functions of the cells, and thus without damage to the cells.
本发明提供了一种光响应组合物,该组合物由一种光响应材料组成,该材料具有随光照变化而产生不同粘附性的特性。光响应组合物可用作培养皿中依赖锚碇生长的细胞的粘附表面。用光照射光响应组合物上特定细胞的位置,只释放附着在该位置上的细胞,释放的细胞可被分类收集。根据本发明,可以通过简单的操作分类收集包括锚定依赖性细胞在内的细胞,同时保持细胞的细胞外基质和膜蛋白,以及细胞的器官特异功能,因此不会对细胞造成损伤。