碱性绿 1 | 633-03-4

• 物质功能分类: 染料及颜料 - 染料 - 碱性染料
• 分子结构分类: 有机化合物 - 苯类化合物 - 苯和取代衍生物
• 中文名称: 碱性绿 1
• 中文别名: 亮绿；碱性绿1；碱性亮绿1/灿烂绿；灿烂绿；碱性亮绿；盐基金沙绿；碱性艳绿；N-[4-[(4-二乙氨基)苯基]苯基亚甲基]-2,5-环己二烯-1-基亚基-N-乙基乙铵硫酸盐；煌绿
• 英文名称: brilliant green
• 英文别名: brillant green；brilliant green dye；[4-[[4-(Diethylamino)phenyl]-phenylmethylidene]cyclohexa-2,5-dien-1-ylidene]-diethylazanium;hydron;sulfate；[4-[[4-(diethylamino)phenyl]-phenylmethylidene]cyclohexa-2,5-dien-1-ylidene]-diethylazanium;hydron;sulfate
• CAS: 633-03-4
• 化学式: C₂₇H₃₃N₂*HO₄S
• 分子量: 482.644
• InChiKey: NNBFNNNWANBMTI-UHFFFAOYSA-M

物化性质

• 熔点：
  210 °C (dec.)(lit.)
• 沸点：
  100.0
• 密度：
  1.1016 (rough estimate)
• 蒸气密度：
  16.6 (vs air)
• 溶解度：
  100克/升
• 最大波长(λmax)：
  625nm, 428nm
• LogP：
  0.765 at 20℃
• 颜色/状态：
  Minute, glistening, golden crystals
• 分解：
  When heated to decomposition it emits very toxic fumes of /nitrogen oxides, ammonia and sulfur oxides/.
• 稳定性/保质期：
  远离氧化物

计算性质

• 辛醇/水分配系数(LogP)：
  4.96
• 重原子数：
  34
• 可旋转键数：
  7
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.3
• 拓扑面积：
  92.1
• 氢给体数：
  1
• 氢受体数：
  5

ADMET

毒理性

• 人类毒性摘录

灿烂的绿色... 已被局部应用于皮肤作为消毒剂，但接触眼睛则严重有害。在一个实例中，尝试用这种染料的1%溶液治疗结膜炎，结果导致了破坏性的角膜炎，伴有前房积脓，并最终因角膜混浊而导致双目失明。

Brilliant green ... has been employed topically on the skin as an antiseptic, but in contact with the eye is severely injurious. In one instance an attempt to treat conjunctivitis with 1% solution of this dye resulted in destructive keratitis with hypopyon and terminated in bilateral blindness due to corneal opacification.

来源:Hazardous Substances Data Bank (HSDB)

毒理性

• 人类毒性摘录

摄食会导致腹泻和腹痛。

Ingestion causes diarrhea & abdominal pain.

来源:Hazardous Substances Data Bank (HSDB)

毒理性

• 人类毒性摘录

湿疹，与龙胆紫和孔雀石绿发生交叉反应。

Eczema, cross-reaction with gentian violet and malachite green.

来源:Hazardous Substances Data Bank (HSDB)

毒理性

• 非人类毒性摘录

在兔眼中实验性地使用这种染料会造成严重伤害。

Experimentally in rabbit eyes the dye is severely injurious.

来源:Hazardous Substances Data Bank (HSDB)

毒理性

• 非人类毒性摘录

Saccharomyces cerevisiae 单倍体菌株15B-II4用于急性实验研究三种芳基甲基染料的毒性和致突变作用，包括亮绿色（C.I. 42040）。测试的所有染料都具有很高的生物活性，其毒性效应包括细胞杀伤和生长抑制。研究表明，这些染料能增加核点突变和呼吸缺乏的细胞质突变的出现频率。

The haploid strain 15B-II4 of Saccharomyces cerevisiae was used to study in an acute experiment the toxic and mutagenic effects of /three/ arilmethan dyes ... /including/ brilliant green (C.I. 42040). ...High biological activity of all the dyes tested was found, based on such toxic effects as cell killing and growth inhibition. ... It was shown that the dyes could incr the frequency of appearance of nuclear point mutations and cytoplasmic mutations of respiratory deficiency.

来源:Hazardous Substances Data Bank (HSDB)

安全信息

• TSCA：
  Yes
• 危险等级：
  6.1(a)
• 危险品标志：
  Xn
• 安全说明：
  S26,S39
• 危险类别码：
  R22,R20/21/22,R36,R36/37/38
• WGK Germany：
  3
• 海关编码：
  3204 13 00
• 危险品运输编号：
  2811
• RTECS号：
  BP6825000
• 包装等级：
  II
• 危险类别：
  6.1(a)
• 危险性防范说明：
  P305+P351+P338
• 危险性描述：
  H302,H319
• 储存条件：
  存放在密封容器内，并放置在阴凉、干燥处。请注意，储存地点需远离氧化剂。

SDS

Name:	Brilliant Green High Purity Biological Stain Material Safety Data Sheet
Synonym:	C.I. 42040; Malachite Green G; Ethyl Green; Emerald Green; Diamond Green G; Fast Green J; Solid Gree
CAS:	633-03-4

Section 1 - Chemical Product MSDS Name:Brilliant Green High Purity Biological Stain Material Safety Data Sheet
Synonym:C.I. 42040; Malachite Green G; Ethyl Green; Emerald Green; Diamond Green G; Fast Green J; Solid Gree

---

Section 2 - COMPOSITION, INFORMATION ON INGREDIENTS

CAS#	Chemical Name	content	EINECS#
633-03-4	Brilliant Green	100	211-190-1

Hazard Symbols: None Listed.
Risk Phrases: None Listed.

---

Section 3 - HAZARDS IDENTIFICATION
EMERGENCY OVERVIEW
Hygroscopic (absorbs moisture from the air).
Potential Health Effects
Eye:
This product contains a cationic dye. Similar dyes have caused permanent injury to the cornea and conjunctiva in documented exposure cases with human or rabbit eyes.
Skin:
Causes skin irritation.
Ingestion:
May cause gastrointestinal irritation with nausea, vomiting and diarrhea. The toxicological properties of this substance have not been fully investigated.
Inhalation:
May cause respiratory tract irritation. The toxicological properties of this substance have not been fully investigated.
Chronic:
No information found.

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Section 4 - FIRST AID MEASURES
Eyes: Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical aid immediately. SPEEDY ACTION IS CRITICAL! Skin:
Get medical aid if irritation develops or persists. Wash clothing before reuse. Flush skin with plenty of soap and water.
Ingestion:
If victim is conscious and alert, give 2-4 cupfuls of milk or water.
Never give anything by mouth to an unconscious person. Get medical aid.
Inhalation:
Remove from exposure and move to fresh air immediately. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical aid.
Notes to Physician:

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Section 5 - FIRE FIGHTING MEASURES
General Information:
As in any fire, wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH (approved or equivalent), and full protective gear. During a fire, irritating and highly toxic gases may be generated by thermal decomposition or combustion. Vapors may be heavier than air. They can spread along the ground and collect in low or confined areas.
Extinguishing Media:
Use alcohol foam, carbon dioxide, or water spray when fighting fires involving this material. In case of fire, use water, dry chemical, chemical foam, or alcohol-resistant foam.

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Section 6 - ACCIDENTAL RELEASE MEASURES
General Information: Use proper personal protective equipment as indicated in Section 8.
Spills/Leaks:
Clean up spills immediately, observing precautions in the Protective Equipment section. Sweep up, then place into a suitable container for disposal. Avoid generating dusty conditions. Remove all sources of ignition. Provide ventilation.

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Section 7 - HANDLING and STORAGE
Handling:
Wash thoroughly after handling. Remove contaminated clothing and wash before reuse. Use only in a well-ventilated area. Avoid contact with eyes, skin, and clothing. Keep container tightly closed. Do not ingest or inhale. Wash clothing before reuse.
Storage:
Store in a cool, dry, well-ventilated area away from incompatible substances. Keep containers tightly closed.

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Section 8 - EXPOSURE CONTROLS, PERSONAL PROTECTION
Engineering Controls:
Facilities storing or utilizing this material should be equipped with an eyewash facility and a safety shower. Use adequate ventilation to keep airborne concentrations low.
Exposure Limits CAS# 633-03-4: Personal Protective Equipment Eyes: Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA's eye and face protection regulations in 29 CFR 1910.133 or European Standard EN166.
Skin:
Wear appropriate protective gloves to prevent skin exposure.
Clothing:
Wear appropriate protective clothing to prevent skin exposure.
Respirators:
A respiratory protection program that meets OSHA's 29 CFR 1910.134 and ANSI Z88.2 requirements or European Standard EN 149 must be followed whenever workplace conditions warrant respirator use.

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Section 9 - PHYSICAL AND CHEMICAL PROPERTIES

Physical State: Solid
Color: green
Odor: odorless
pH: Not available.
Vapor Pressure: Negligible.
Viscosity: Not available.
Boiling Point: Not available.
Freezing/Melting Point: Decomposes.
Autoignition Temperature: Not applicable.
Flash Point: Not applicable.
Explosion Limits, lower: Not available.
Explosion Limits, upper: Not available.
Decomposition Temperature: 210 deg C
Solubility in water: Moderately soluble in water.
Specific Gravity/Density: Not available.
Molecular Formula: C27H33N2.HSO4
Molecular Weight: 385.2444

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Section 10 - STABILITY AND REACTIVITY
Chemical Stability:
Stable under normal temperatures and pressures.
Conditions to Avoid:
Incompatible materials, moisture.
Incompatibilities with Other Materials:
Strong oxidizing agents.
Hazardous Decomposition Products:
Nitrogen oxides, carbon monoxide, oxides of sulfur, carbon dioxide, nitrogen.
Hazardous Polymerization: Will not occur.

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Section 11 - TOXICOLOGICAL INFORMATION
RTECS#:
CAS# 633-03-4: BP6825000 LD50/LC50:
Not available.
Carcinogenicity:
Brilliant Green - Not listed by ACGIH, IARC, or NTP.
Other:
See actual entry in RTECS for complete information.

---

Section 12 - ECOLOGICAL INFORMATION
Other No information available.

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Section 13 - DISPOSAL CONSIDERATIONS
Dispose of in a manner consistent with federal, state, and local regulations.

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Section 14 - TRANSPORT INFORMATION

IATA
Not regulated as a hazardous material.
IMO
Not regulated as a hazardous material.
RID/ADR
Not regulated as a hazardous material.

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Section 15 - REGULATORY INFORMATION

European/International Regulations
European Labeling in Accordance with EC Directives
Hazard Symbols: Not available.
Risk Phrases:
Safety Phrases:
S 45 In case of accident or if you feel unwell, seek
medical advice immediately (show the label where
possible).
WGK (Water Danger/Protection)
CAS# 633-03-4: 2
Canada
CAS# 633-03-4 is listed on Canada's DSL List.
CAS# 633-03-4 is not listed on Canada's Ingredient Disclosure List.
US FEDERAL
TSCA
CAS# 633-03-4 is listed on the TSCA inventory.

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SECTION 16 - ADDITIONAL INFORMATION
N/A

制备方法与用途

碱性绿1简介

碱性绿1又称为灿烂绿，是一种属于碱性三苯甲烷类的染料。在水产养殖中，它常用作杀菌剂和抗寄生虫药。由于三苯甲烷类具有致突变、致畸性和致癌作用，欧美及我国已严禁在水产养殖中使用此类染料。如果池塘水体和土壤受到含灿烂绿的污染，在后续养殖过程中容易导致鱼体内形成残留物。因此，鱼类养殖时需对池塘水体和土壤进行有效的监控。

应用 化学性质

碱性绿1是一种绿色闪金光砂状物，溶于冷水和热水，水溶液呈绿色；极易溶于乙醇，呈绿色；在浓硫酸中呈黄色，稀释后又呈现绿色。其水溶液与氢氧化钠作用会生成淡绿色沉淀。

用途

• 碱性绿1可用于麻、蚕丝、腈纶织物和草制品的染色。
• 它也用于纸张的染色及制造色淀。
• 在皮革和竹木等材料上也可进行着色处理。

生产方法

碱性绿1由N,N-二乙基苯胺与苯甲醛在尿素的存在下缩合，再通过二氧化铅和盐酸氧化，而后用硫酸钠脱铅、碳酸钠中和、硫酸溶解结晶等步骤制得。生产过程中消耗的原料为：N,N-二乙基苯胺515kg/t，苯甲醛325kg/t，二氧化铅745kg/t。

安全性 类别

碱性绿1属于有毒物品类别。

急性毒性

口服：大鼠LDL0 为10毫克/公斤；腹腔注射小鼠LDL0 为5毫克/公斤。

刺激数据

• 皮肤接触（人）：2毫克/2天 轻度刺激。
• 皮肤接触（豚鼠）：6毫克/3天 轻度刺激。

可燃性危险特性

碱性绿1可燃，燃烧时会产生有毒的氮氧化物、硫氧化物和氨烟雾。

储运特性

应保持通风、低温且干燥的储存条件。

灭火剂

使用干粉、泡沫、沙土或二氧化碳以及雾状水进行灭火。

反应信息

• 作为反应物：
  描述：

  碱性绿 1 在 盐酸 、 水 、 锌 作用下， 生成 苯基双-(对二乙氨基苯)甲烷
  参考文献：
  名称：

  Doebner, Justus Liebigs Annalen der Chemie, 1883, vol. 217, p. 248

  摘要：

  DOI：
• 作为产物：
  描述：

  Brilliantgruen 在 盐酸 、 zinc(II) chloride 作用下， 生成 碱性绿 1
  参考文献：
  名称：

  Doebner, Justus Liebigs Annalen der Chemie, 1883, vol. 217, p. 248

  摘要：

  DOI：

文献信息

• Glycoluril‐Derived Molecular Clips are Potent and Selective Receptors for Cationic Dyes in Water
  作者：Nengfang She、Damien Moncelet、Laura Gilberg、Xiaoyong Lu、Vladimir Sindelar、Volker Briken、Lyle Isaacs

  DOI：10.1002/chem.201601796

  日期：2016.10.17

  Molecular clip 1 remains monomeric in water and engages in host–guest recognition processes with suitable guests. We report the Ka values for 32 1⋅guest complexes measured by 1H NMR, UV/Vis, and fluorescence titrations. The cavity of 1 is shaped by aromatic surfaces of negative electrostatic potential and therefore displays high affinity and selectivity for planar and cationic aromatic guests that

  分子夹1在水中仍然是单体，并与合适的客人一起参与宿主-客体识别过程。我们报告了通过1 H NMR，UV / Vis和荧光滴定法测得的32个1⋅客体配合物的K a值。1的腔体由负电势的芳族表面形成，因此对平面和阳离子芳族客体显示出高亲和力和选择性，这使其与CB [ n ]受体相区别，后者比脂族客体更喜欢脂肪族。静电作用在识别过程中起着主要作用，在此过程中，铵离子和C = O的1个基团之间可能发生离子-偶极相互作用。情况下，SO之间3 -基团的1和在来宾悬垂阳离子基团，和空腔内1由阳离子-π相互作用。宿主1对具有大的平面芳族表面（例如萘二酰亚胺NDI +和per二酰亚胺PDI +）和衍生自a啶的阳离子染料（例如亚甲基蓝和天蓝色A）表现出较高的亲和力。通过比较类似的中性和阳离子客体（例如，亚甲基紫与亚甲基蓝；喹啉与N-甲基喹啉鎓； a啶与N-甲基ac啶；中性红与中性红H +）可以确定阳离子-π相
• Reagents and methods for direct labeling of nucleotides
  申请人：Naleway John J.

  公开号：US20130150254A1

  公开（公告）日：2013-06-13

  The present invention provides systems and methods for production of activatable diazo-derivatives for use in labeling nucleotides. Labeling nucleotides is accomplished by contacting a stable hydrazide derivative of a detectable moiety with an activating polymer reagent which is used to directly label the nucleotide sample. Labeling occurs on the phosphate backbone of the nucleotide which does not perturb hybridization of the labeled nucleotide with its anti-sense strand. Since the method involves direct labeling, all types of nucleotides can be labeled without prior amplification or alteration.

  本发明提供了用于生产可激活重氮衍生物以用于标记核苷酸的系统和方法。通过将可检测基团的稳定的肼酰肼衍生物与用于直接标记核苷酸样品的活化聚合物试剂接触来完成核苷酸的标记。标记发生在核苷酸的磷酸骨架上，不会干扰标记核苷酸与其反义链的杂交。由于该方法涉及直接标记，所有类型的核苷酸都可以在不经过扩增或改变的情况下进行标记。
• D-AMPHETAMINE COMPOUNDS, COMPOSITIONS, AND PROCESSES FOR MAKING AND USING THE SAME
  申请人：KemPharm, Inc.

  公开号：US20200131130A1

  公开（公告）日：2020-04-30

  Disclosed are d-amphetamine compounds and compositions comprising at least one organic acid covalently bound to d-amphetamine, a salt thereof, a derivative thereof, or a combination thereof. Methods of making and using the same are also disclosed.

  揭示了d-安非他明化合物和组合物，其中至少有一种有机酸以共价键结合到d-安非他明，其盐，衍生物或其组合物。还公开了制备和使用这些化合物的方法。
• Antimicrobial Compounds
  申请人：BIOMERIEUX

  公开号：US20170158720A1

  公开（公告）日：2017-06-08

  An antimicrobial compound, as well as the salts, derivatives and analogues thereof, said compound being represented by the general formula (I): wherein R 1 represents a peptide part P1 or a peptide part P2.

  一种抗微生物化合物，以及其盐、衍生物和类似物，所述化合物由通式（I）表示： 其中R1代表肽部分P1或肽部分P2。
• ALKALI-DEVELOPABLE PHOTOSENSITIVE RESIN COMPOSITION AND BETA-DIKETONE COMPOUND
  申请人：Yamada Takashi

  公开号：US20100129753A1

  公开（公告）日：2010-05-27

  An alkali developable photosensitive resin composition contains (J) a photopolymerizable unsaturated compound having a structure resulting from the addition reaction of (B) a compound having a β-diketone moiety or a compound having a β-ketoester group to the (meth)acryloyl group of (A) a compound having at least two (meth)acryloyl groups and a hydroxyl group and subsequent esterification of the hydroxyl group of the resulting addition product with (C) a polybasic acid anhydride. The compound having a β-diketone moiety is preferably a novel β-diketone compound represented by general formula (I): wherein R 1 is a C1-C20 alkyl group; R 2 represents R 11 , OR 11 , COR 11 , SR 11 , CONR 12 R 13 , or CN; R 11 , R 12 , and R 13 are each hydrogen, a C1-C20 alkyl group, etc.; a is 0 to 3; and b is 0 to 4.

  一种可由碱显影的光敏树脂组合物，含有（J）一种光聚合不饱和化合物，该化合物的结构是由（B）含β-二酮基团或含β-酮酯基团的化合物与（A）含至少两个(甲基)丙烯酰基团和一个羟基的化合物中的(甲基)丙烯酰基团发生加成反应，并随后将所得加成产物的羟基与（C）多元酸酐进行酯化反应得到的。含β-二酮基团的化合物最好是表示为通用公式（I）的一种新型β-二酮化合物：其中R1是C1-C20烷基团；R2代表R11、OR11、COR11、SR11、CONR12R13或CN；R11、R12和R13分别是氢、C1-C20烷基团等；a是0到3；b是0到4。

表征谱图