5-(5-chlorothiophen-2-yl)-3-((2-nitrophenoxy)methyl)isoxazole | 1277100-20-5

• 分子结构分类: 有机化合物 - 苯类化合物 - 苯和取代衍生物
• 英文名称: 5-(5-chlorothiophen-2-yl)-3-((2-nitrophenoxy)methyl)isoxazole
• CAS: 1277100-20-5
• 化学式: C₁₄H₉ClN₂O₄S
• 分子量: 336.755
• InChiKey: FMXWKIMFDSIPPC-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  4.54
• 重原子数：
  22.0
• 可旋转键数：
  5.0
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.07
• 拓扑面积：
  78.4
• 氢给体数：
  0.0
• 氢受体数：
  6.0

反应信息

• 作为反应物：
  描述：

  5-(5-chlorothiophen-2-yl)-3-((2-nitrophenoxy)methyl)isoxazole 在 tin(II) chloride dihdyrate 作用下， 以 N,N-二甲基甲酰胺 为溶剂， 生成 2-[[5-(5-Chlorothiophen-2-yl)-1,2-oxazol-3-yl]methoxy]aniline
  参考文献：
  名称：

  Biarylmethoxy isonipecotanilides as potent and selective inhibitors of blood coagulation factor Xa

  摘要：

  New chloro-substituted biarylmethoxyphenyl piperidine-4-carboxamides were synthesized and assayed in vitro as inhibitors of the blood coagulation enzymes factor Xa (fXa) and thrombin. An investigation of effects of the amidine and isopropyl groups attached at the piperidine nitrogen and 5-(halogenoaryl)isoxazol-3-yl groups as biaryl substituents led us to identify new compounds which proved to be selective fXa inhibitors, with inhibition constants in the low nanomolar range. The most potent compound 21e, that incorporates 2-Cl-thiophen-5-yl group as the P1 motif and 1-isopropylpiperidine P4 group, inhibited fXa with K-i value of 0.3 nM and very high selectivity over thrombin and some other tested serine proteases, achieving moderate levels of anticoagulant activity in the low micromolar range, as assessed by the prothrombin time clotting assay (PT2 = 3.30 mu M). Based on reliable docking simulations, molecular modeling provided a rationale for interpreting structure-activity relationships. The predicted binding modes highlighted the structural requirements for addressing the subsites S1 and S4 of the fXa enzyme. (C) 2010 Elsevier B.V. All rights reserved.

  DOI：

  10.1016/j.ejps.2010.11.010
• 作为产物：
  描述：

  5-(5-氯噻吩-2-基)异恶唑-3-羧酸乙酯 在 sodium tetrahydroborate 、 偶氮二甲酸二异丙酯 、 三苯基膦 作用下， 以 四氢呋喃 、 乙醇 为溶剂， 反应 52.0h， 生成 5-(5-chlorothiophen-2-yl)-3-((2-nitrophenoxy)methyl)isoxazole
  参考文献：
  名称：

  Biarylmethoxy isonipecotanilides as potent and selective inhibitors of blood coagulation factor Xa

  摘要：

  New chloro-substituted biarylmethoxyphenyl piperidine-4-carboxamides were synthesized and assayed in vitro as inhibitors of the blood coagulation enzymes factor Xa (fXa) and thrombin. An investigation of effects of the amidine and isopropyl groups attached at the piperidine nitrogen and 5-(halogenoaryl)isoxazol-3-yl groups as biaryl substituents led us to identify new compounds which proved to be selective fXa inhibitors, with inhibition constants in the low nanomolar range. The most potent compound 21e, that incorporates 2-Cl-thiophen-5-yl group as the P1 motif and 1-isopropylpiperidine P4 group, inhibited fXa with K-i value of 0.3 nM and very high selectivity over thrombin and some other tested serine proteases, achieving moderate levels of anticoagulant activity in the low micromolar range, as assessed by the prothrombin time clotting assay (PT2 = 3.30 mu M). Based on reliable docking simulations, molecular modeling provided a rationale for interpreting structure-activity relationships. The predicted binding modes highlighted the structural requirements for addressing the subsites S1 and S4 of the fXa enzyme. (C) 2010 Elsevier B.V. All rights reserved.

  DOI：

  10.1016/j.ejps.2010.11.010

文献信息

• β-D-Glucosyl Conjugates of Highly Potent Inhibitors of Blood Coagulation Factor Xa Bearing 2-Chorothiophene as a P1 Motif
  作者：Gianfranco Lopopolo、Modesto de Candia、Luigi Panza、Maria Rosaria Romano、Marcello Diego Lograno、Francesco Campagna、Cosimo Altomare

  DOI：10.1002/cmdc.201200224

  日期：2012.9

  synthesized a novel O‐glucoside of the recently reported potent factor Xa (fXa) inhibitor 1, which bears a 5‐chlorothien‐2‐yl moiety and 1‐isopropylpiperidine as fragments that bind the S1 and S4 enzyme pockets, respectively. A β‐D‐glucosyl unit was conjugated through an ether‐linked C3‐alkyl spacer to the central phenyl ring of 1. The synthesized β‐D‐glucose‐based compound 16 achieved picomolar inhibitory

  我们合成了最近报道的强效因子Xa（fXa）抑制剂1的新型O-葡萄糖苷，该抑制剂具有5-氯噻吩-2-基部分和1-异丙基哌啶，分别作为结合S1和S4酶口袋的片段。β- D-葡萄糖基单元通过醚连接的C3-烷基间隔基与1的中心苯环共轭。合成的基于β- D-葡萄糖的化合物16对人fXa的皮摩尔抑制能力（K i = 60 p M）和对凝血酶和其他丝氨酸蛋白酶的高选择性。除了氯噻吩基S1粘合剂外，ΔG的增益也很大起因于加入质子化1-异丙基哌（ΔΔ ģ = 29.7-30.5摩尔千焦-1），它应绑定到通过高效阳离子-π和C芳香族S4口袋 H⋅⋅⋅π相互作用。相反，-C 3 -烷基连接的葡萄糖片段，这是有可能朝向酶结合位点外的溶剂定向，改善Δ ģ平均2.9-3.8千焦摩尔-1。化合物16显示出亚微摩尔的体外抗凝血活性，如通过凝血酶原时间（PT）和评估凝血活酶时间（APTT）活化凝血中的混合人血浆测定（PT