(E)-2-hydrazono-3-methyl-2,3-dihydrobenzo[d]thiazole | 1128-67-2

• 物质功能分类: 化学试剂 - 有机试剂 - 腙
• 分子结构分类: 有机化合物 - 有机杂环化合物 - 苯并噻唑类
• 英文名称: (E)-2-hydrazono-3-methyl-2,3-dihydrobenzo[d]thiazole
• 英文别名: 3-methyl-3H-benzothiazol-2-one hydrazone；3-methylbenzothiazolone-2-hydrazone-6-carboxylic acid；N-methylbenzothiazolone hydrazone dihydrochloride；(2E)-3-methyl-1,3-benzothiazol-2(3H)-one hydrazone hydrochloride；(E)-(3-methyl-1,3-benzothiazol-2-ylidene)hydrazine
• CAS: 1128-67-2；62082-97-7
• 化学式: C₈H₉N₃S
• 分子量: 179.246
• InChiKey: PHOLIFLKGONSGY-CSKARUKUSA-N

物化性质

• 熔点：
  144°C
• 沸点：
  342.9±25.0 °C(Predicted)
• 密度：
  1.1939 (rough estimate)

计算性质

• 辛醇/水分配系数(LogP)：
  1.9
• 重原子数：
  12
• 可旋转键数：
  0
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.12
• 拓扑面积：
  66.9
• 氢给体数：
  1
• 氢受体数：
  3

安全信息

• 危险类别码：
  R36,R25
• 危险品运输编号：
  UN 2811
• 海关编码：
  2934200090
• 安全说明：
  S26,S36,S45

反应信息

• 作为反应物：
  描述：

  (E)-2-hydrazono-3-methyl-2,3-dihydrobenzo[d]thiazole 、 2-羟基-1-萘甲醛 以 甲醇 为溶剂， 反应 3.0h， 以78%的产率得到(1-((Z)-(((E)-3-methylbenzo[d]thiazol-2(3H)-ylidene))hydrazono)methyl)naphthalen-2-ol
  参考文献：
  名称：

  A Benzothiazole-Based Fluorescence Turn-on Sensor for Copper(II)

  摘要：

  合成了一种新的苯并噻唑基化学传感器 BTN（1-((Z)-((E)-3-甲基苯并[d]噻唑-2(3H)-亚基)肼基)甲基)萘-2-醇），用于检测 Cu2+。无论是否存在其他阳离子，BTN 都能以从无色到黄色的 "关-开 "荧光反应检测 Cu2+。Cu2+ 的检测限被确定为 3.3 μM。根据约伯图和 ESI-MS 分析，BTN 与 Cu2+ 的结合率为 1:1。理论计算解释了 BTN 对 Cu2+ 的感应特征，这可能是由于内部电荷转移和螯合增强荧光过程造成的。

  DOI：

  10.1007/s10895-021-02752-x

文献信息

• Method of measuring substance in sample using a redox reaction
  申请人：Kyoto Daiichi Kagaku Co., Ltd.

  公开号：US20020025546A1

  公开（公告）日：2002-02-28

  A highly reliable method of measuring an analyte in a sample using a redox reaction. In this method, a tetrazolium compound is added to a sample prior to the redox reaction so as to eliminate the influence of any reducing substance in the sample, then a reducing substance or an oxidizing substance derived from the analyte is formed, the quantity of the formed substance derived from the analyte is measured by the redox reaction, and the quantity of the analyte is determined from the quantity of the formed substance derived from the analyte. As the tetrazolium compound, for example, 2-(4-iodophenyl)-3-(2,4-dinitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium salt can be used.

  一种高度可靠的测量样品中分析物的方法，使用氧化还原反应。在该方法中，在氧化还原反应之前向样品中添加四唑化合物以消除样品中任何还原物质的影响，然后形成从分析物中衍生的还原物质或氧化物质，通过氧化还原反应测量从分析物中衍生的物质的数量，并从中确定分析物的数量。例如，可以使用2-（4-碘苯基）-3-（2,4-二硝基苯基）-5-（2,4-二硫酚基）-2H-四唑盐作为四唑化合物。
• Determination method of biological component and reagent kit used therefor
  申请人：——

  公开号：US20020119507A1

  公开（公告）日：2002-08-29

  The present invention provides novel glutathione-dependent formaldehyde dehydrogenase that makes possible quantitative measurement of formaldehyde by cycling reaction, and a determination method of formaldehyde and biological components, such as creatinine, creatine, homocysteine and the like, which produces formaldehyde as a reaction intermediate. In addition, the present invention provides a reagent kit for the above-mentioned determination method. The present invention provides a novel determination method of a homocysteine using transferase utilizing homocysteine and other compound as a pair of substrates. Particularly, the present invention provides a determination method of homocysteine which includes bringing betaine-homocysteine methyltransferase and dimethylglycine oxidase into contact with a sample and measuring produced hydrogen peroxide or formaldehyde. Moreover, the present invention provides novel dimethylglycine oxidase stable to thiol compound, which is suitably used for the measurement. The present invention provides a reagent kit used for any of the above-mentioned determination methods of homocysteines.

  本发明提供了一种新型的谷胱甘肽依赖性甲醛脱氢酶，它使得通过循环反应定量测量甲醛成为可能，并提供了一种测定甲醛和生物成分（如肌酐、肌酸、同型半胱氨酸等）的方法，该方法产生甲醛作为反应中间体。此外，本发明还提供了用于上述测定方法的试剂盒。本发明提供了一种新型的利用同型半胱氨酸和其他化合物作为一对底物的转移酶测定同型半胱氨酸的方法。特别是，本发明提供了一种同型半胱氨酸的测定方法，其中包括将甜菜碱-同型半胱氨酸甲基转移酶和二甲基甘氨酸氧化酶与样品接触，并测量产生的过氧化氢或甲醛。此外，本发明还提供了一种对巯基化合物稳定的新型二甲基甘氨酸氧化酶，适用于测量。本发明提供了用于上述同型半胱氨酸测定方法的试剂盒。
• Method of assaying bolld component by using whole blood and measurement kit
  申请人：Umegae Yoshihiki

  公开号：US20100075352A1

  公开（公告）日：2010-03-25

  [Problem] In quickly assaying a blood component interfered by glucose and/or its derivative on the bedside or in a clinic or in assaying the same by a patient in his/her own home, there has been required an assay method wherein the whole blood can be used as a sample as such without resorting to a centrifuge or the like. [Means For Solving Problems] A method of assaying a blood component to be used for assaying a blood component interfered by glucose and/or its derivative, characterized by comprising bringing the whole blood into contact with a substance capable of converting glucose and/or its derivative into another substance not interfering the assay and, simultaneously or subsequently, separating blood cells; a device to be used in the assay method; and a kit containing this device.

  【问题】在床边或诊所中快速检测被葡萄糖和/或其衍生物干扰的血液成分，或者在患者自己的家中进行检测，需要一种检测方法，可以使用全血作为样本，而不需要离心机或类似设备。【解决问题的方法】一种检测血液成分的方法，用于检测被葡萄糖和/或其衍生物干扰的血液成分，其特征在于将全血与能够将葡萄糖和/或其衍生物转化为不干扰检测的另一种物质接触，并同时或随后分离血细胞；用于该检测方法的设备；以及包含该设备的试剂盒。
• L-glutamic acid oxidase, its production, and its use
  申请人：Yamasa Shoyu Kabushiki Kaisha

  公开号：EP0097949A2

  公开（公告）日：1984-01-11

  The present invention consists of the L-glutamic acid oxidase which is an L-amino acid oxidase catalyzing the oxidative deamination of the a-amino group of L-glutamic acid in the presence of water and oxygen to form a-ketoglutaric acid, ammonia and hydrogen peroxide, and having a very high substrate specificity for L-glutamic acid substantially without acting on L-glutamine and L-histidine and also a high stability, a microbiological method of production thereof, an analytical method for assay of L-glutamic acid in a sample to be analyzed by the use of this enzyme, a reagent for analysis to practice the analytical method, a kit for analysis comprising said reagent, and a biosensor employing said enzyme.

  本发明包括L-谷氨酸氧化酶，它是一种L-氨基酸氧化酶，在水和氧的存在下催化L-谷氨酸的a-氨基氧化脱氨，生成a-酮戊二酸、氨和过氧化氢，对L-谷氨酸具有很高的底物特异性，基本上不作用于L-谷氨酰胺和L-组氨酸，而且具有很高的稳定性、一种生产该酶的微生物学方法、一种利用该酶分析样品中 L-谷氨酸的分析方法、一种用于实施该分析方法的分析试剂、一种包含上述试剂的分析试剂盒以及一种利用上述酶的生物传感器。
• A rapid assaying method for guanase
  申请人：MARUHO CO., LTD.

  公开号：EP0075894A1

  公开（公告）日：1983-04-06

  The guanase activity in body fluids such as blood serum can rapidly and accurately assayed by (I) decomposing guanine with the guanase in the specimen to xanthine and ammonia at pH 7-9, preferably at pH 8, (II) decomposing the xanthine formed by former step I with xanthine oxidase to uric acid and hydrogen peroxide, reacting the reactant solution of the former step II with 3-methyl-2-benzothiazolinonehydrazone, an aniline derivative such as N-N-di-lower-alkylaniline and peroxidase, and finally measuring the optical absorption of the reactant solution of the step III at 570-600 nm. The all steps can be completed within 15 minutes. Therefore, this assay is adoptable for automatic assay of guanase by usual clinically available automatic analysers.

  通过以下方法可以快速准确地测定血清等体液中鸟嘌呤酶的活性：(I) 在 pH 值为 7-9 时，最好是在 pH 值为 8 时，用标本中的鸟嘌呤酶将鸟嘌呤分解成黄嘌呤和氨；(II) 用黄嘌呤氧化酶将前一步 I 生成的黄嘌呤分解成尿酸和过氧化氢、将前述步骤 II 的反应物溶液与 3-甲基-2-苯并噻唑啉酮腙、苯胺衍生物如 N-N-二低烷基苯胺和过氧化物酶反应，最后在 570-600 纳米波长处测量步骤 III 反应物溶液的光吸收。所有步骤可在 15 分钟内完成。因此，临床上常用的自动分析仪可采用这种方法自动检测鸟苷酸酶。