[Ru(ethylenediamine-N,N,N',N'-tetraacetate)(adenine)](1-) | 578742-86-6

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 英文名称: [Ru(ethylenediamine-N,N,N',N'-tetraacetate)(adenine)](1-)
• CAS: 578742-86-6
• 化学式: C₁₅H₁₇N₇O₈Ru
• 分子量: 524.412
• InChiKey: UYSRKUVPYQNSLU-UHFFFAOYSA-J

反应信息

• 作为产物：
  描述：

  K[Ru^III(ethylenediaminetetraacetate)Cl].2H₂O 、 腺嘌呤 在 水 作用下， 以 水 为溶剂， 生成 [Ru(ethylenediamine-N,N,N',N'-tetraacetate)(adenine)](1-)
  参考文献：
  名称：

  Reactivity of [ Ru^III(edta)(H₂O)]^–with nucleic bases, nucleosides and DNA (calf-thymus) in aqueous solution (etda = ethylenediamine-N,N,N′,N′-tetraacetate)

  摘要：

  Reactions of [Ru-III(edta)(H2O)](-) (edta = ethylenediamine-N,N.N',N'-tetraacetate) with L' (L' = adenine, adenosine, cytosine, cytidine or thymine) and calf-thymus DNA were studied by spectrophotometric. electrochemical and kinetic methods. Spectral features of the substituted product [Ru-III(edta)L']- complexes were characterised by a strong ligand-to-metal charge transfer (l.m.c.t.) band in the UV region (293-309 nm). The E(1/2) values for the Ru-III-Ru-II couple for [Ru-III(edta)L'](-) are in the range -0.28 to -0.15 V (vs. saturated calomel electrode). Equilibration kinetics of complexation of [Ru-III(edta)(H2O)](-) with L' have been investigated using stopped-flow methods at 25 degrees C and pH 5.2. Equilibrium constants determined kinetically are in good agreement with those obtained spectrophotometrically. The reactivity of L' towards aqua-substitution of [Ru-III(edta)(H2O)](-) complex follows the sequence adenine > adenosine > cytosine > cytidine. On the basis of spectrophotometric, electrochemical and kinetic data and comparing the reactivity of [Ru-III(edta)(H2O)](-) with DNA bases and DNA (calf-thymus) itself it is proposed that the interaction of [Ru-III(edta)(H2O)](-) with DNA takes place through the adenine base unit in a kinetically preferred pathway.

  DOI：

  10.1039/dt9950002497

文献信息

• The substitution mechanism of [Ruiii(edta)(H2O)]− with DNA bases, nucleoside and nucleotides in aqueous solution revisited
  作者：Debabrata Chatterjee、Anannya Mitra、Mohamed S. A. Hamza、Rudi van Eldik

  DOI：10.1039/b108232a

  日期：——

  The substitution reactions of [RuIII(edta)(H2O)]− (edta = ethylenediaminetetraacetate) with adenine, adenosine and the corresponding 5′-nucleotides (Nu), viz. adenosine-5′-monophosphate (AMP), adenosine-5′-diphosphate (ADP) and adenosine-5′-triphosphate (ATP), have been studied kinetically as a function of nucleotide concentration at various temperatures (5 to 30 °C) at a fixed pH of 4.6 to contribute to the mechanistic understanding of the binding of adenine base nucleotides. Based on the kinetic results, it is suggested that the binding of the 5′-nucleotides (AMP, ADP and ATP) takes place in a rapid nucleophile concentration-dependent step, followed by a concentration-independent ring-closure reaction. Kinetic data and activation parameters have been interpreted in terms of an associative mechanism and discussed in reference to the data reported before.

  [RuIII(edta)(H2O)]⁻（edta = 乙二胺四乙酸）与腺嘌呤、腺苷及其相应的5'-核苷酸（Nu），即腺苷-5'-单磷酸（AMP）、腺苷-5'-二磷酸（ADP）和腺苷-5'-三磷酸（ATP）的替代反应已在不同温度（5到30°C）下以核苷酸浓度为变量，在固定pH 4.6的条件下进行了动力学研究，以促进对腺嘌呤碱基核苷酸结合机制的理解。基于动力学结果，建议5'-核苷酸（AMP、ADP和ATP）的结合发生在一个快速的、依赖于亲核体浓度的步骤，然后是一个浓度无关的环闭合反应。动力学数据和活化参数已根据一种亲和机制进行了解释，并与之前报告的数据进行了讨论。