VHL(OH) | 16881-26-8

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 英文名称: VHL(OH)
• 英文别名: Val-His-Leu；(2S)-2-[[(2S)-2-[[(2S)-2-amino-3-methylbutanoyl]amino]-3-(1H-imidazol-5-yl)propanoyl]amino]-4-methylpentanoic acid
• CAS: 16881-26-8
• 化学式: C₁₇H₂₉N₅O₄
• 分子量: 367.448
• InChiKey: KVRLNEILGGVBJX-IHRRRGAJSA-N

物化性质

• 沸点：
  749.728±60.00 °C(Press: 760.00 Torr)(predicted)
• 密度：
  1.209±0.06 g/cm3(Temp: 25 °C; Press: 760 Torr)(predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  -1.5
• 重原子数：
  26
• 可旋转键数：
  10
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.65
• 拓扑面积：
  150
• 氢给体数：
  5
• 氢受体数：
  6

反应信息

• 作为反应物：
  描述：

  VHL(OH) 、 t-butyl 2-((6-(4-((1r,3r)-3-(3-chloro-4-cyanophenoxy)-2,2,4,4-tetramethylcyclobutyl)(methoxy)benzamido)hex-5-yn-1-yl)oxy)acetate 在 三氟乙酸 、 N,N-二异丙基乙胺 、 N-[(dimethylamino)-3-oxo-1H-1,2,3-triazolo[4,5-b]pyridin-1-yl-methylene]-N-methylmethanaminium hexafluorophosphate 作用下， 以 二氯甲烷 、 N,N-二甲基甲酰胺 为溶剂， 反应 4.0h， 以47%的产率得到(2R,4R)-1-((S)-2-(2-((6-(4-(((1r,3r)-3-(3-chloro-4-cyanophenoxy)-2,2,4,4-tetramethylcyclobutyl)(methoxy)benzamido)hex-5-yn-1-)oxy)acetamido)-3,3-dimethylbutyryl)-4-hydroxy-N-(S)-1-(4-(4-methylthiazol-5-)phenyl)ethyl)tetrahydropyrrole-2-amide
  参考文献：
  名称：

  EP3957633

  摘要：

  公开号：

文献信息

• PROCESS FOR PRODUCING a-GLYCOSYLATED DIPEPTIDE AND METHOD OF ASSAYING a-GLYCOSYLATED DIPEPTIDE
  申请人：KIKKOMAN CORPORATION

  公开号：EP1679378A1

  公开（公告）日：2006-07-12

  The present invention relates to a method for producing α-glycated dipeptide, which comprises causing protease to act on N-terminal-glycated peptide or N-terminal-glycated protein. The present invention further relates to a method for determining the amount of α-glycated dipeptide, which comprises causing a fructosyl peptide oxidase to act on the α-glycated dipeptide obtained by the above method and then determining the amount of the thus generated hydrogen peroxide. According to the present invention, a method for producing α-glycated dipeptide is provided, which enables the simple, rapid, and efficient production of α-glycated dipeptide from glycated protein or glycated peptide. Furthermore, according to the present invention, a method for determining the amount of α-glycated dipeptide is provided, which enables to determine the amount of α-glycated dipeptide in a highly precise manner within a short time period.

  本发明涉及一种生产α-糖化二肽的方法，它包括使蛋白酶作用于N-末端糖化的肽或N-末端糖化的蛋白质。本发明还涉及一种测定α-糖化二肽量的方法，该方法包括使果糖基肽氧化酶作用于通过上述方法获得的α-糖化二肽，然后测定由此产生的过氧化氢的量。根据本发明，提供了一种生产α-糖化二肽的方法，该方法能够从糖化蛋白质或糖化肽中简单、快速、高效地生产α-糖化二肽。此外，根据本发明，还提供了一种测定α-糖化二肽量的方法，该方法能够在短时间内以高度精确的方式测定α-糖化二肽的量。
• REAGENT CONTAINING PROTEASE REACTION PROMOTER AND/OR COLORANT STABILIZER
  申请人：Asahi Kasei Pharma Corporation

  公开号：EP1788081A1

  公开（公告）日：2007-05-23

  [PROBLEMS] To provide a protease reaction promoter being superior in usefulness to the known protease reaction promoters, a stabilizer for a colorant to be used in detecting an enzymatic reaction, a method therefor and a reagent and a method for measuring a glycosylated protein and the ratio of a glycosylated protein with the use of the same. [MEANS FOR SOLVING PROBLEMS] A protease reaction promoter which contains at least one member selected from among an acetate group-containing compound or its salt, N-acyltaurine or its salt and a polyoxyethylene alkyl ether sulfate or its salt; a method of promoting a protease reaction by using this protease reaction promoter, a stabilizer reagent containing cyclodextrin for stabilizing a colorant to be used in detecting an enzymatic reaction; a method of stabilization via the coexistence of cyclodextrin with the above colorant; and a reagent and a method for measuring a glycosylated protein and the ratio of a glycosylated protein with the use of the protease reaction promoter and/or the reagent containing cyclodextrin as described above.

  [问题] 提供一种在实用性上优于已知蛋白酶反应促进剂的蛋白酶反应促进剂、一种用于检测酶反应的着色剂的稳定剂、一种用于此的方法以及一种使用该促进剂和稳定剂测量糖基化蛋白质和糖基化蛋白质比率的试剂和方法。[解决问题的方法]一种蛋白酶反应促进剂，它含有至少一种选自含醋酸基的化合物或其盐、N-酰基牛磺酸或其盐和聚氧乙烯烷基醚硫酸盐或其盐中的成员；一种通过使用这种蛋白酶反应促进剂促进蛋白酶反应的方法，一种含有环糊精的稳定剂试剂，用于稳定检测酶反应中使用的着色剂；通过环糊精与上述着色剂共存进行稳定的方法；以及使用上述蛋白酶反应促进剂和/或含有环糊精的试剂测量糖基化蛋白质和糖基化蛋白质比率的试剂和方法。
• METHOD FOR MEASURING GLYCATED HEMOGLOBIN
  申请人：Hitachi Chemical Diagnostics Systems Co., Ltd.

  公开号：EP3636767A1

  公开（公告）日：2020-04-15

  A method is provided for measuring glycated hemoglobin in a hemoglobin-containing sample which comprises: adding an enzyme that catalyzes a reaction of oxidizing glycated amino acid or glycated peptide to generate hydrogen peroxide without oxidizing the glycated hemoglobin, to the hemoglobin-containing sample to generate hydrogen peroxide; eliminating the generated hydrogen peroxide; adding an enzyme that catalyzes a reaction of oxidizing glycated hemoglobin to generate hydrogen peroxide thereto to generate hydrogen peroxide; and measuring the generated hydrogen peroxide.

  提供了一种测量含血红蛋白样品中糖化血红蛋白的方法，该方法包括在含血红蛋白样品中加入催化氧化糖化氨基酸或糖化肽反应生成过氧化氢而不氧化糖化血红蛋白的酶，生成过氧化氢；消除生成的过氧化氢；加入催化氧化糖化血红蛋白反应生成过氧化氢的酶，生成过氧化氢；测量生成的过氧化氢。
• PROCESS FOR PRODUCING ALPHA-GLYCATED DIPEPTIDES BY PROTEOLYSIS
  申请人：KIKKOMAN CORPORATION

  公开号：EP1679378B1

  公开（公告）日：2017-03-22
• Measurement method using enzymes
  申请人：ARKRAY, Inc.

  公开号：EP2639586B1

  公开（公告）日：2016-06-29