BiBH | 1203719-63-4

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 英文名称: BiBH
• 英文别名: N,N'-bis(2-bromoisobutyryl)hexane-1,6-diamine
• CAS: 1203719-63-4
• 化学式: C₁₄H₂₆Br₂N₂O₂
• 分子量: 414.181
• InChiKey: QOHJNHUHKANOLA-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  3.13
• 重原子数：
  20.0
• 可旋转键数：
  9.0
• 环数：
  0.0
• sp3杂化的碳原子比例：
  0.86
• 拓扑面积：
  58.2
• 氢给体数：
  2.0
• 氢受体数：
  2.0

反应信息

• 作为产物：
  描述：

  1,6-己二胺 、 2-溴-2-甲基丙酰溴 以 二氯甲烷 为溶剂， 以31%的产率得到BiBH
  参考文献：
  名称：

  Nanoparticles from block copolymer encapsulating Re(phen) complexes as bifunctional agents for cell imaging and gene transfection

  摘要：

  利用嵌段共聚物封装 Re（phen）复合物，成功构建了发光双功能核壳纳米粒子，用于在活细胞中同时进行细胞成像和基因转染。

  DOI：

  10.1039/b912659g

文献信息

• Influence of block sequences in polymer vectors for gene transfection in vitro and toxicity assessment of zebrafish embryos in vivo
  作者：Yang Yao、Dao-Fu Feng、Yi-Pan Wu、Qi-Jun Ye、Lei Liu、Xin-Xin Li、Sen Hou、Yan-Lian Yang、Chen Wang、Lei Li、Xi-Zeng Feng

  DOI：10.1039/c0jm03728a

  日期：——

  In this study, a triblock copolymer PDMAEMA-b-poly(PEGMA)-b-PDMAEMA (DED) and a diblock copolymer PDMAEMA-b-poly(PEGMA) (DE) were synthesized by atom transfer radical polymerization (ATRP) with the initiators bis(2-bromoisobutyryl) hexanediamide (BiBH) and ethyl 2-bromoisobutyrate (EBiB), respectively. Their molecular weights and chemical compositions were almost the same with only difference being in the block sequences. The influence of the block copolymer structures on the gene transfection results was investigated systematically. Gel retardation assay, atomic force microscopy (AFM) observation, particle size and zeta potential measurements with dynamic light scattering (DLS) were used to characterize the properties of the polymer/pDNA complexes. DED showed moderate aggregation behavior with far less cytotoxicity to in vitro and in vivo to zebrafish than DE, which indicates that DED would be a promising vector for gene therapeutic applications.

  本研究分别以双（2-溴异丁酰）己二胺（BiBH）和 2-溴异丁酸乙酯（EBiB）为引发剂，通过原子转移自由基聚合（ATRP）合成了三嵌段共聚物 PDMAEMA-b-poly(PEGMA)-b-PDMAEMA (DED)和二嵌段共聚物 PDMAEMA-b-poly(PEGMA) (DE)。它们的分子量和化学成分几乎相同，唯一不同的是嵌段序列。我们系统地研究了嵌段共聚物结构对基因转染结果的影响。凝胶延缓试验、原子力显微镜（AFM）观察、粒度和 zeta 电位测量以及动态光散射（DLS）被用来表征聚合物/pDNA 复合物的特性。DED 显示出适度的聚集行为，对体外和体内斑马鱼的细胞毒性远低于 DE，这表明 DED 将是一种很有前景的基因治疗应用载体。