cis-2,4-dihydroxy-3,4-dihydrophenanthrene | 61616-82-8

• 分子结构分类: 有机化合物 - 苯类化合物 - 菲及其衍生物
• 英文名称: cis-2,4-dihydroxy-3,4-dihydrophenanthrene
• 英文别名: (3R,4S)-3,4-dihydrophenanthrene-3,4-diol
• CAS: 61616-82-8
• 化学式: C₁₄H₁₂O₂
• 分子量: 212.248
• InChiKey: FOTICWSJABVKPW-TZMCWYRMSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  2
• 重原子数：
  16
• 可旋转键数：
  0
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.14
• 拓扑面积：
  40.5
• 氢给体数：
  2
• 氢受体数：
  2

反应信息

• 作为产物：
  描述：

  菲 在 S_. lividans mycelium harboring pIJ₆₀₂₁-phdABCD 作用下， 以 various solvent(s) 为溶剂， 生成 cis-2,4-dihydroxy-3,4-dihydrophenanthrene
  参考文献：
  名称：

  Biotransformation of Phenanthrene and 1-Methoxynaphthalene with Streptomyces lividans Cells Expressing a Marine Bacterial Phenanthrene Dioxygenase Gene Cluster

  摘要：

  海洋细菌诺卡氏菌中的 phdABCD 基因簇。菌株 KP7 编码多组分酶菲双加氧酶。编码铁硫蛋白大亚基α的phdA、编码其小亚基β的phdB、编码铁氧还蛋白的phdC和编码铁氧还蛋白还原酶的phdD被替换，使得前面的开放阅读框的终止密码子与起始密码子重叠。以下基因。这个经过操纵的 phdABCD 基因簇位于高拷贝数载体 pIJ6021 中硫链丝菌素诱导型启动子 PtipA 的下游，并引入革兰氏阳性、土壤栖息的丝状细菌浅青紫链霉菌中。重组变青链球菌细胞将菲转化为顺式二醇形式，通过紫外光谱数据和HPLC性质确定为顺式-3,4-二羟基-3,4-二氢菲，并与正品样品进行对比。这种生物转化非常有效地进行。 200 μM 和 2 mM 菲分别在 6 小时和 32 小时内几乎完全转化为其顺式二醇形式。此外，还发现携带phdABCD基因簇的S. lividans细胞可以将1-甲氧基萘转化为两种产物，除了8-甲氧基-1,2-二氢-1之外，还鉴定出这两种产物是8-甲氧基-2-萘酚。 ,2-萘二醇的 EI-MS、1H-和 13C-NMR 光谱数据。

  DOI：

  10.1271/bbb.65.1774

文献信息

• Novel polypeptides and methods for their use
  申请人：——

  公开号：US20030022335A1

  公开（公告）日：2003-01-30

  The invention provides an NDO or NDO related complex comprising at least one alpha-subunit polypeptide that comprises: 1) a substituted amino acid at the position corresponding to position 352 in NDO, 2) a substituted amino acid at the position corresponding to position 201, 202, 260, 316, 351, 358, 362, or 366 in NDO, or 3) a substituted amino acid at the position corresponding to position 352 in NDO, and a substituted amino acid at the position corresponding to position 201, 202, 260, 316, 351, 358, 362, or 366 in NDO; or a catalytically active fragment thereof. The invention also provides DNA encoding such polypeptides, host cells augmented by such DNA, and methods for using the enzymes or host cells to provide useful and novel synthons. The invention also provides novel compounds prepared with the complexes or methods of the invention.

  本发明提供了一种包含至少一个α-亚基多肽的NDO或NDO相关复合物，其中该α-亚基多肽包括：1）在与NDO的352位相对应的位置上的取代氨基酸，2）在与NDO的201、202、260、316、351、358、362或366位相对应的位置上的取代氨基酸，或3）在与NDO的352位相对应的位置上的取代氨基酸和在与NDO的201、202、260、316、351、358、362或366位相对应的位置上的取代氨基酸；或其催化活性片段。本发明还提供了编码这种多肽的DNA，通过这种DNA增强的宿主细胞以及使用这些酶或宿主细胞提供有用和新颖合成物的方法。本发明还提供了使用该复合物或本发明的方法制备的新型化合物。
• Probe compound for detecting and isolating enzymes and means and methods using the same
  申请人：Helmholtz-Zentrum für Infektionsforschung GmbH

  公开号：EP2230312A1

  公开（公告）日：2010-09-22

  The present invention relates to a probe compound that can comprise any substrate or metabolite of an enzymatic reaction in addition to an indicator component, such as, for example, a fluorescence dye, or the like. Moreover, the present invention relates to means for detecting enzymes in form of an array, which comprises any number of probe compounds of the invention which each comprise a different metabolite of interconnected metabolites representing the central pathways in all forms of life. Moreover, the present invention relates to a method for detecting enzymes involving the application of cell extracts or the like to the array of the invention which leads to reproducible enzymatic reactions with the substrates. These specific enzymatic reactions trigger the indicator (e.g. a fluorescence signal) and bind the enzymes to the respective cognate substrates. Moreover, the invention relates to means for isolating enzymes in form of nanoparticles coated with the probe compound of the invention. The immobilisation of the cognate substrates or metabolites on the surface of nanoparticles by means of the probe compounds allows capturing and isolating the respective enzyme, e.g. for subsequent sequencing.

  本发明涉及一种探针化合物，它可以包括酶反应的任何底物或代谢物，此外还包括指示成分，例如荧光染料或类似物。此外，本发明还涉及以阵列形式检测酶的方法，该阵列由任意数量的本发明探针化合物组成，每种探针化合物由代表所有生命形式中中心途径的相互关联的代谢物中的不同代谢物组成。此外，本发明还涉及一种检测酶的方法，该方法涉及将细胞提取物或类似物应用于本发明的阵列，从而导致与底物发生可重复的酶反应。这些特定的酶反应会触发指示剂（如荧光信号），并将酶与各自的同源底物结合。此外，本发明还涉及以涂覆有本发明探针化合物的纳米颗粒形式分离酶的方法。通过探针化合物将同源底物或代谢物固定在纳米颗粒表面，可以捕获和分离相应的酶，例如用于后续测序。
• Efficient synthesis of non-K-region trans-dihydrodiols of polycyclic aromatic hydrocarbons from o-quinones and catechols
  作者：Karl L. Platt、Franz Oesch

  DOI：10.1021/jo00150a027

  日期：1983.1
• Nucleic Acids and Proteins and Methods for Making and Using Them
  申请人：Mathur Eric J.

  公开号：US20100011456A1

  公开（公告）日：2010-01-14

  The invention provides polypeptides, including enzymes, structural proteins and binding proteins, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. Polypeptides, including enzymes and antibodies, and nucleic acids of the invention can be used in industrial, experimental, food and feed processing, nutritional and pharmaceutical applications, e.g., for food and feed supplements, colorants, neutraceuticals, cosmetic and pharmaceutical needs.
• NUCLEIC ACIDS AND PROTEINS AND METHODS FOR MAKING AND USING THEM
  申请人：Chang Hwai

  公开号：US20110296543A1

  公开（公告）日：2011-12-01

  The invention provides polypeptides, including enzymes, structural proteins and binding proteins, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. Polypeptides, including enzymes and antibodies, and nucleic acids of the invention can be used in industrial, experimental, food and feed processing, nutritional and pharmaceutical applications, e.g., for food and feed supplements, colorants, neutraceuticals, cosmetic and pharmaceutical needs. Polypeptides of the invention can be used in food processing, brewing, bath additives, alcohol production, peptide synthesis, enantioselectivity, hide preparation in the leather industry, waste management and animal degradation, silver recovery in the photographic industry, medical treatment, silk degumming, biofilm degradation, biomass conversion to a biofuel (e.g., a bioethanol, biomethanol, biopropanol or biobutanol, a biodiesel, etc.), biodefense, antimicrobial agents and disinfectants, personal care and cosmetics, biotech reagents, in corn wet milling and pharmaceuticals such as digestive aids and anti-inflammatory (anti-phlogistic) agents.