Functional mono- and dinuclear peptide complexes featuring chemospecific κS- or η6-coordination of organometallic half-sandwich fragments
摘要:
Treatment of the dipeptides H-Tyr-Trp-OH, H-Trp-Tyr-OH, H-Trp-Phe-OH and H-Phe-Tyr-OH with [(T,(6)- cymene)Ru(acetone)(3)].(CF3SO3)(2) (1) in CF3COOH leads to chemospecific eta(6)-coordination in the order of electron-donating ability Trp > Tyr > Phe in respective complexes of the type [(eta(6)-cymene)Ru(eta(6) -dipeptide)](CF3SO3)(2) (3-6) after 24-36 h at 20degreesC. An analogous thermodynamic chemospecificity was also established for the complexes [(eta(5) -Cp*)Ir(eta(6)-dipeptide)](CF3SO3)(2) (7, H-Phe-Trp-OH; 8, H-Phe-Tyr-OH). However, a time-dependent 1H NMR study of the reaction between [(eta(5) -Cp*)Ir(acetone)(3)] (CF3SO3)(2) (2) and H-Tyr-Phe-OH confirms an initial kinetic preference for eta(6)-coordination of the C-terminal arene, as is also apparent from product mixtures obtained after treating 2 with H-Trp-Phe-OH or H-Trp-Tyr-OH for 1-2 d at 50 degreesC. Dinuclear sandwich complexes of the type [(eta(5)-Cp *)Ir(mu(2)-H-Met-X-OH-eta(6)-X:kappa(2)N(M), S)Pt(en)](CF3SO3)(4) (9-11, X = Phe, Tyr, Trp) can be prepared in CF3COOH following initial N-terminal kappa(2)N(M), S chelation of the (en)Pt-II fragment by the methionine residue of the dipeptide. Chemospecific kappaS coordination of the [(eta(5)-Cp *)Ir(dppZ)](2+) fragment in [(eta(5)-Cp*)Ir(dppz) (H-2-HArg-Met-NH2-kappaS)](4+) (12), [{(eta(5)-Cp*)Ir(dppz)}(2)(mu-H-2-Met -Met-OH-kappaS:kappaS')](5+) (13) and [(eta(5)-Cp*)Ir(dppz)(mu-H-Gly-Met-OH-kappaS:kappaN(G))Pt(terpy)](4+) (14) affords effective metallointercalators for DNA with binding constants K-b in the range 1.3-3.3 x 10(6) M-1 and marked melting temperature shifts DeltaT(m) of respectively 10.2, 15.2 and 11.2 degreesC. (C) 2003 Elsevier B.V. All rights reserved.
Nutrient compositions comprise an aqueous solution of at least one dipeptide with an N-terminal amino acid selected from glutamine, aspargine, tyrosine, tryptophan, and aspargine.
The C-terminal amino acid is alanine, glycine, tryptophan, arginine, proline or serine. The nutritional compositions contain from about 0.1 to 25.0 percent by weight of oligopeptides, preferably about 0.5 to 5.0 percent by weight.
营养组合物包含至少一种二肽的水溶液,其 N 端氨基酸选自谷氨酰胺、天门冬氨酸、酪氨酸、色氨酸和天门冬氨酸。
C 端氨基酸为丙氨酸、甘氨酸、色氨酸、精氨酸、脯氨酸或丝氨酸。营养组合物含有约 0.1%至 25.0%(按重量计)的低聚肽,最好是约 0.5%至 5.0%(按重量计)。
ADDITIVE FOR UNDIFFERENTIATION MAINTAINING MEDIUM
申请人:Ajinomoto Co., Inc.
公开号:EP3604503A1
公开(公告)日:2020-02-05
The present invention provides a medium for culturing pluripotency stem cells, containing L-tryptophan or L-tryptophan derivative at a concentration of not less than 176 µM.
PRECISE ESTIMATION OF GLOMERULAR FILTRATION RATE FROM MULTIPLE BIOMARKERS
申请人:THE JOHNS HOPKINS UNIVERSITY
公开号:US20170276669A1
公开(公告)日:2017-09-28
The present invention relates to the field of nephrology. More specifically, the present invention provides methods and compositions useful for more precisely estimating glomerular filtration rate (GFR). In a specific embodiment, a method for calculating the estimated glomerular filtration rate (eGFR) in a patient comprises the steps of (a) measuring the level of one or more metabolites using mass spectrometry from a blood sample obtained from the patient; and (b) calculating the eGFR using an algorithm that utilizes the measured levels of the one or more metabolites.
[EN] PROTEIN GLYCATION SUPPRESSANT<br/>[FR] SUPPRESSEUR DE GLYCATION DE PROTÉINE<br/>[JA] タンパク質糖化抑制剤