Benzyloxycarbonyl-glutamine-glycine | 2718-40-3

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 英文名称: Benzyloxycarbonyl-glutamine-glycine
• 英文别名: 2-[[5-amino-5-oxo-2-(phenylmethoxycarbonylamino) pentanoyl] amino]acetic acid；benzyloxycarbonyl-L-glutamylglycine；benzyloxycarbonyl-glutamylglycine；Z-Gln-Gly；2-[[5-amino-5-oxo-2-(phenylmethoxycarbonylamino)pentanoyl]amino]acetic acid
• CAS: 2718-40-3
• 化学式: C₁₅H₁₉N₃O₆
• mdl: MFCD02184598
• 分子量: 337.332
• InChiKey: SOUXAAOTONMPRY-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -0.4
• 重原子数：
  24
• 可旋转键数：
  10
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.333
• 拓扑面积：
  148
• 氢给体数：
  4
• 氢受体数：
  6

反应信息

• 作为反应物：
  描述：

  Benzyloxycarbonyl-glutamine-glycine 在 N,N'-二异丙基碳二亚胺 作用下， 以 N,N-二甲基甲酰胺 为溶剂， 反应 32.0h， 生成 Z-QG-UTP
  参考文献：
  名称：

  NUCLEOSIDE TRIPHOSPHATE DERIVATIVE, NUCLEIC ACID PROBE, MULTILABELED NUCLEIC ACID PROBE, METHOD FOR PRODUCTION OF MULTILABELED NUCLEIC ACID PROBE, AND METHOD FOR DETECTION OF TARGET NUCLEIC ACID

  摘要：

  公开号：

  EP2301946B1

文献信息

• [EN] LYSINE CONJUGATED IMMUNOGLOBULINS<br/>[FR] IMMUNOGLOBULINES CONJUGUÉES À LA LYSINE
  申请人：EISAI R&D MAN CO LTD

  公开号：WO2017213267A1

  公开（公告）日：2017-12-14

  Provided herein are conjugated immunoglobulins and methods for generating conjugated immunoglobulins using a microbial transglutaminase.

  本文提供了共轭免疫球蛋白及利用微生物转谷氨酰胺酶生成共轭免疫球蛋白的方法。
• Site-Specific Conjugation to Native and Engineered Lysines in Human Immunoglobulins by Microbial Transglutaminase
  作者：Jared L. Spidel、Benjamin Vaessen、Earl F. Albone、Xin Cheng、Arielle Verdi、J. Bradford Kline

  DOI：10.1021/acs.bioconjchem.7b00439

  日期：2017.9.20

  residues in an antibody based on the hypothesis that the lower specificity of MTG for acyl acceptor lysines may result in the transamidation of multiple native lysine residues, thereby yielding heterogeneous products. We investigated the utilization of native IgG lysines as acyl acceptor sites for glutamine-based acyl donor substrates. Of the approximately 80 lysines in multiple recombinant IgG monoclonal

  迄今为止，利用微生物转谷氨酰胺酶（MTG）产生位点特异性抗体-药物偶联物（ADC）的研究重点在于抗体中工程改造的酰基供体谷氨酰胺残基的转酰胺基化，其假设是MTG对酰基受体赖氨酸的特异性较低可能导致多个天然赖氨酸残基的转酰胺基化，从而产生异质产物。我们调查了天然IgG赖氨酸作为基于谷氨酰胺的酰基供体底物的酰基受体位点的利用。在多种重组IgG单克隆抗体（mAb）中大约80个赖氨酸中，没有一个被转酰胺基化。由于重组单克隆抗体由于在生产细胞宿主中被羧肽酶B裂解而缺乏C端Lys447，我们探讨了通过添加C末端氨基酸来阻断Lys447的裂解是否会导致各种酰基供体底物对Lys447的转酰胺基作用。在位置448处存在任何非酸性，非脯氨酸氨基酸残基的情况下，MTG有效地转酰胺化了Lys447。整个抗体中的赖氨酸扫描诱变进一步揭示了重链和轻链恒定区中的多个转酰胺基位。另外，对Fab'片段中的铰链区的诱变的扫描
• Method for producing protein-nucleic acid conjugate, and method for detecting target substance
  申请人：KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION

  公开号：US09388454B2

  公开（公告）日：2016-07-12

  Provided is a method for producing a nucleic acid probe that can detect a target substance with good sensitivity. A method for producing a nucleic acid probe, comprising: a 3′-terminal addition step of adding at least one nucleoside triphosphate derivative having a glutamine (Gln) residue or a lysine (Lys) residue to the 3′-terminal of a nucleic acid using a 3′-terminal addition enzyme which adds a nucleotide to the 3′-terminal of a nucleic acid, and a labeling compound binding step of either binding a labeling compound having a lysine (Lys) residue and containing a labeling moiety to the glutamine (Gln) residue using a transglutaminase (TGase), or binding a labeling compound having a glutamine (Gln) residue and containing a labeling moiety to the lysine (Lys) residue using a transglutaminase (TGase).

  提供的是一种制备能够以良好的灵敏度检测目标物质的核酸探针的方法。一种制备核酸探针的方法，包括：使用一种3'端加成酶向核酸的3'端添加至少一种具有谷氨酰胺（Gln）残基或赖氨酸（Lys）残基的核苷酸三磷酸衍生物进行3'端加成步骤，以及使用一种转谷氨酰胺酶（TGase）将具有赖氨酸（Lys）残基和含有标记基团的标记化合物结合到谷氨酰胺（Gln）残基上，或者将具有谷氨酰胺（Gln）残基和含有标记基团的标记化合物结合到赖氨酸（Lys）残基上的标记化合物结合步骤。
• METHOD FOR PRODUCING PROTEIN-NUCLEIC ACID COMPLEX, AND METHOD FOR DETECTING TARGET SUBSTANCE
  申请人：Kyushu University, National University Corporation

  公开号：EP2865762A1

  公开（公告）日：2015-04-29

  Provided is a method for producing a nucleic acid probe that can detect a target substance with good sensitivity. A method for producing a nucleic acid probe, comprising: a 3'-terminal addition step of adding at least one nucleoside triphosphate derivative having a glutamine (Gin) residue or a lysine (Lys) residue to the 3'-terminal of a nucleic acid using a 3'-terminal addition enzyme that can add a nucleotide to the 3'-terminal of a nucleic acid; and a labeling compound binding step of binding a labeling compound having a lysine (Lys) residue and containing a labeling moiety to the glutamine (Gin) residue using a transglutaminase (TGase) or binding a labeling compound having a glutamine (Gin) residue and containing a labeling moiety to the lysine (Lys) residue using a transglutaminase (TGase).

  本发明提供了一种能灵敏检测目标物质的核酸探针的生产方法。一种生产核酸探针的方法，包括3'-末端添加步骤：使用可将核苷酸添加到核酸 3'- 末端的 3'- 末端添加酶，将至少一种具有谷氨酰胺（Gin）残基或赖氨酸（Lys）残基的三磷酸核苷衍生物添加到核酸的 3'- 末端；以及标记化合物结合步骤：使用转谷氨酰胺酶（TGase）将具有赖氨酸（Lys）残基且含有标记分子的标记化合物与谷氨酰胺（Gin）残基结合，或使用转谷氨酰胺酶（TGase）将具有谷氨酰胺（Gin）残基且含有标记分子的标记化合物与赖氨酸（Lys）残基结合。
• Enzymatic conjugation of polypeptides
  申请人：Innate Pharma

  公开号：US10071169B2

  公开（公告）日：2018-09-11

  One embodiment relates to methods for the enzymatic functionalization of immunoglobulins, in particular with drugs. Also disclosed herein are linking reagents, functionalized antibodies, pharmaceutical compositions, and method of treating disease and/or conditions.

  一个实施方案涉及免疫球蛋白的酶功能化方法，特别是与药物的酶功能化。本文还公开了连接试剂、功能化抗体、药物组合物以及治疗疾病和/或病症的方法。