3’-O-aminothymidine-5’-triphosphate | 1159990-37-0

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘧啶核苷酸
• 英文名称: 3’-O-aminothymidine-5’-triphosphate
• 英文别名: 3'-O-amino-thymidine-5'-triphosphate；[[(2R,3S,5R)-3-aminooxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate
• CAS: 1159990-37-0
• 化学式: C₁₀H₁₈N₃O₁₄P₃
• 分子量: 497.186
• InChiKey: WTXJWVBIEUJGOO-XLPZGREQSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -7.1
• 重原子数：
  30
• 可旋转键数：
  9
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.6
• 拓扑面积：
  254
• 氢给体数：
  6
• 氢受体数：
  15

反应信息

• 作为产物：
  描述：

  3'-O-(Fmoc)amino-thymidine-5'-triphosphate 在 乙酸–三乙胺 、 乙腈 作用下， 以 哌啶 、 N,N-二甲基甲酰胺 为溶剂， 反应 0.95h， 以to give 3′-O-amino-thymidine-5′-triphosphate as a colorless foam的产率得到3’-O-aminothymidine-5’-triphosphate
  参考文献：
  名称：

  Method for sequencing DNA and RNA by synthesis

  摘要：

  本发明涉及核酸化学领域，更具体地涉及核酸类似物的组合物和过程领域。更具体地说，本发明涉及通过合成测序对寡核苷酸进行测序的组合物，以及利用这些组合物进行合成测序的过程。最具体地，本发明揭示了一种物质组成，即5'-核糖核苷酸和2'-脱氧核糖核苷酸的三磷酸盐，其中3'-OH基团被α构型的3'-ONHR基团所取代，其中R是H或CH3基团。还揭示了这些三磷酸盐，其中核碱基通过连接体携带报告基团，例如可以用于单个或多个复制DNA测序的荧光物种，或者可以通过超微观察可视化的标签。还揭示了使用这些组合物进行测序合成的过程。

  公开号：

  US07544794B1

文献信息

• [EN] REAGENTS FOR REVERSIBLY TERMINATING PRIMER EXTENSION<br/>[FR] RÉACTIFS UTILISÉS POUR L'INTERRUPTION RÉVERSIBLE D'EXTENSION D'AMORCE
  申请人：BENNER STEVEN ALBERT

  公开号：WO2010110775A1

  公开（公告）日：2010-09-30

  This invention relates to the field of nucleic acid chemistry, more specifically to the field of compositions of matter that comprise triphosphates of modified 2'-deoxynucleosides and oligonucleotides that are formed when these are appended to the 3'-end of a primer, wherein said modifications comprise NH2 moiety attached to their 3'-hydroxyl group and a fluorescent species in a form of a tag affixed to the nucleobase via a linker that can be cleaved. Such compositions and their associated processes enable and improve the sequencing of oligonucleotides using a strategy of cyclic reversible termination, as outlined in US Patent 6,664,079. Most specifically, the invention concerns compositions of matter that are 5'-triphosphates of ribo- and 2'- deoxyribonucleosides carrying detectable tags and oligonucleotides that might be derived from them. The invention also concerns processes wherein a DNA polymerase, RNA polymerase, or reverse transcriptase synthesizes said oligonucleotides via addition of said triphosphates to a primer.

  这项发明涉及核酸化学领域，更具体地涉及包括改性2'-脱氧核苷酸三磷酸和寡核苷酸的物质组合的领域，当这些物质附加到引物的3'-末端时形成，其中这些改性包括连接到它们的3'-羟基的NH2基团和以可切割的方式通过连接器固定到核碱基上的荧光物种的标签。这种组合物及其相关的过程使得并改善了使用循环可逆终止策略进行寡核苷酸测序，正如美国专利6,664,079中所概述的那样。具体而言，该发明涉及的是包含可检测标签的核糖和2'-脱氧核糖核苷酸的5'-三磷酸和可能由它们衍生的寡核苷酸的物质组合。该发明还涉及DNA聚合酶、RNA聚合酶或逆转录酶通过将这些三磷酸添加到引物而合成所述寡核苷酸的过程。
• Reagents for reversibly terminating primer extension
  申请人：Benner Steven A.

  公开号：US20110275124A1

  公开（公告）日：2011-11-10

  This invention relates to the field of nucleic acid chemistry, more specifically to the field of compositions of matter that comprise triphosphates of modified 2′-deoxynucleosides and oligonucleotides that are formed when these are appended to the 3′-end of a primer, wherein said modifications comprise NH 2 moiety attached to their 3′-hydroxyl group and a fluorescent species in a form of a tag affixed to the nucleobase via a linker that can be cleaved. Such compositions and their associated processes enable and improve the sequencing of oligonucleotides using a strategy of cyclic reversible termination, as outlined in U.S. Pat. No. 6,664,079. Most specifically, the invention concerns compositions of matter that are 5′-triphosphates of ribo- and 2′-deoxyribonucleosides carrying detectable tags and oligonucleotides that might be derived from them. The invention also concerns processes wherein a DNA polymerase, RNA polymerase, or reverse transcriptase synthesizes said oligonucleotides via addition of said triphosphates to a primer.

  本发明涉及核酸化学领域，更具体地涉及包含经过修饰的2'-去氧核苷酸三磷酸盐和寡核苷酸的物质组成，当它们附加到引物的3'-端时形成，其中所述修饰包括连接到它们的3'-羟基上的NH2基团和通过可断链的连接器固定在核碱基上的荧光物种。这些组成物及其相关的过程使得并改进了使用循环可逆终止策略对寡核苷酸进行测序，如美国专利号6,664,079所概述的。最具体地，本发明涉及载有可检测标记的核糖核苷酸和2'-去氧核糖核苷酸的5'-三磷酸盐以及可能从它们中派生出的寡核苷酸的物质组成。本发明还涉及DNA聚合酶、RNA聚合酶或反转录酶通过向引物添加所述三磷酸盐来合成所述寡核苷酸的过程。
• Method for Preparing 3'-O-Amino-2'-Deoxyribonucleoside-5'-Triphosphate
  申请人：DNA Script

  公开号：US20210214382A1

  公开（公告）日：2021-07-15

  The invention relates to a method for preparing 3′-O-amino-2′-deoxyribonucleoside-5′-triphosphate. It also relates to a solid support functionalized with at least one N-hydroxyphthalimide moiety and uses thereof for protecting 3′-hydroxy group of a 2′-deoxyribonucleoside during synthesis of a nucleoside or a derivative thereof.
• US7544794B1
  申请人：——

  公开号：US7544794B1

  公开（公告）日：2009-06-09
• US8034923B1
  申请人：——

  公开号：US8034923B1

  公开（公告）日：2011-10-11