methyl 3β-hydroxy-6-oxo-5α-cholanoate | 116262-85-2

• 分子结构分类: 有机化合物 - 脂质和类脂质分子 - 类固醇和类固醇衍生物
• 英文名称: methyl 3β-hydroxy-6-oxo-5α-cholanoate
• 英文别名: 3β-hydroxy-6-oxo-5α-cholanoic acid-(24)-methyl ester；3β-Hydroxy-6-oxo-5α-cholansaeure-(24)-methylester；methyl (4R)-4-[(3S,5S,8S,9S,10R,13R,14S,17R)-3-hydroxy-10,13-dimethyl-6-oxo-1,2,3,4,5,7,8,9,11,12,14,15,16,17-tetradecahydrocyclopenta[a]phenanthren-17-yl]pentanoate
• CAS: 116262-85-2
• 化学式: C₂₅H₄₀O₄
• 分子量: 404.59
• InChiKey: PDEKSQPGUZKWQD-NDPNZEMMSA-N

物化性质

• 熔点：
  151.5-152.5 °C
• 沸点：
  505.7±25.0 °C(Predicted)
• 密度：
  1.085±0.06 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  5
• 重原子数：
  29
• 可旋转键数：
  5
• 环数：
  4.0
• sp3杂化的碳原子比例：
  0.92
• 拓扑面积：
  63.6
• 氢给体数：
  1
• 氢受体数：
  4

反应信息

• 作为反应物：
  描述：

  methyl 3β-hydroxy-6-oxo-5α-cholanoate 在 chromium(VI) oxide 、 溶剂黄146 作用下， 生成 methyl 3,6-dioxo-5α-cholan-24-oate
  参考文献：
  名称：

  Hattori; Yamada, Yakugaku Zasshi/Journal of the Pharmaceutical Society of Japan, 1941, vol. 61, p. 464,466; dtsch. Ref. S. 151

  摘要：

  DOI：
• 作为产物：
  描述：

  猪去氧胆酸 在 盐酸 、 甲醇 、 重铬酸吡啶 、 potassium carbonate 、 三苯基膦 、 偶氮二甲酸二乙酯 作用下， 以 甲醇 、 二氯甲烷 、 水 为溶剂， 生成 methyl 3β-hydroxy-6-oxo-5α-cholanoate
  参考文献：
  名称：

  Synthesis of 5α-cholestan-6-one derivatives and their inhibitory activities of NO production in activated microglia: Discovery of a novel neuroinflammation inhibitor

  摘要：

  Glial activation-mediated neuroinflammation plays a pivotal role in the process of several neuroinflammatory diseases including stroke, Alzheimer's diseases, Parkinson's diseases, multiple sclerosis and ischemia. Inhibition of microglial activation may ameliorate neuronal degeneration under the inflammatory conditions. In the present study, a number of 5 alpha-cholestan-6-one derivatives were prepared and the anti-inflammatory effects of these compounds were evaluated in LPS-stimulated BV-2 microglia cells. Those derivatives were synthesized from readily available hyodeoxycholic acid (1). Among the tested compounds, several analogs (16-18, 25, 35, 38) exhibited potent inhibitory activities on nitric oxide production with no or weak cell toxicity. Compound 16 also significantly suppressed the expression of TNF-alpha, interleukin (IL)-1 beta, cyclooxygenase (COX-2) as well as inducible nitric oxide synthase (iNOS) in LPS-stimulated BV-2 microglia cells. In addition, compound 16 markedly reduced infarction volume in a focal ischemic mice model. (C) 2013 Elsevier Ltd. All rights reserved.

  DOI：

  10.1016/j.bmcl.2013.12.055

文献信息

• Potential bile acid metabolites. 20. A new synthetic route to stereoisomeric 3,6,-dihydroxy- and 6-hydroxy-5α-cholanoic acids
  作者：Takashi Iida、Tamaaki Tamaru、Frederic C. Chang、Toshifumi Niwa、Junichi Goto、Toshio Nambara

  DOI：10.1016/0039-128x(93)90039-p

  日期：1993.8

  An improved procedure for the syntheses of stereoisomeric 3,6-dihydroxy- and 6-hydroxy-5 alpha-cholanoic acids (and their methyl esters) is described. The principal reactions employed are those reported in the preceding paper of this series, with the commercially available hyodeoxycholic acid as starting material. The final step in the procedure is the reduction of the key 5 alpha C-6 ketones with

  描述了一种合成立体异构体3,6-二羟基-和6-羟基-5α-胆酸（及其甲酯）的改进方法。所采用的主要反应是该系列先前论文中报道的反应，以市售的猪去氧胆酸为起始原料。该方法的最后一步是用立体选择性赤道试剂Li / NH3 / MeOH或轴向试剂Zn（BH4）2还原关键的5个αC-6酮。通过薄层色谱，高效液相色谱和气-液色谱迁移率，1H和13C核磁共振谱对制备的6-单羟基化和3,6-二羟基化立体异构体的分析结果进行讨论。 5 beta系列的相应化合物。
• Kimura, Hoppe-Seyler's Zeitschrift fur Physiologische Chemie, 1937, vol. 248, p. 280,283
  作者：Kimura

  DOI：——

  日期：——
• Isolation and analysis of fluP, a gene associated with hyphal growth and sporulation in Aspergillus parasiticus
  作者：R. Zhou、R. Rasooly、J.E. Linz

  DOI：10.1007/s004380000335

  日期：2000.11

  Aflatoxins (AF) are polyketide-derived mycotoxins that frequently contaminate food and feed crops, causing health risks to animals and humans. The fluP gene was cloned by screening an Aspergillus parasiticus genomic DNA library with a cDNA probe encoding part of a polyketide synthase (PKS), the 6-methylsalicylic acid synthase (MSAS) from Penicillium patulum. FluP was hypothesized to function as a PKS in AF biosynthesis. The predicted amino acid sequence of FluP demonstrated a high degree of identity to MSAS (55%), moderate identity to another fungal PKS protein encoded by wA from A. nidulans (22%) and low identity (<5%) to fungal fatty acid synthase (FAS) proteins. Disruption of fluP in A. parasiticus resulted in the loss of fluP transcript, a 3- to 4-fold reduction in hyphal growth rate, the appearance of a fluffy, cotton-like hyphal morphology, reduction or elimination of asexual spores and spore-bearing structures, and a twofold reduction in aflatoxin accumulation. Removal of selective pressure on fluP knockout transformants resulted in frequent reversion (10%) to the wild-type genotype and phenotype, establishing a direct link between gene disruption and the associated phenotype. The data suggest that fluP encodes a novel PKS associated with hyphal growth and cell development (sporulation). whose activity indirectly influences aflatoxin accumulation in A. parasiticus.