5-amino-2,3-dihydro-1,4-phthalazinedione sodium | 20666-12-0

• 物质功能分类: 生物 - 细胞培养 - 添加剂
• 分子结构分类: 有机化合物 - 有机杂环化合物 - 二氮杂萘
• 英文名称: 5-amino-2,3-dihydro-1,4-phthalazinedione sodium
• 英文别名: sodium luminol；5-amino-2,3-dihydro-phthalazine-1,4-dione; sodium-salt；5-Amino-2,3-dihydro-phthalazin-1,4-dion; Natrium-Salz；Natrium-3-amino-phthalhydrazid；Sodium 5-amino-4-oxo-3,4-dihydrophthalazin-1-olate；sodium;5-amino-4-oxo-3H-phthalazin-1-olate
• CAS: 20666-12-0
• 化学式: C₈H₆N₃O₂*Na
• 分子量: 199.144
• InChiKey: GBOXDRMCEVPUSV-UHFFFAOYSA-M

物化性质

• 熔点：
  319-320 C
• 稳定性/保质期：
  如果遵照规格使用和储存，则不会分解。避免接触酸、氧化物、还原剂以及碱。

计算性质

• 辛醇/水分配系数(LogP)：
  -3.42
• 重原子数：
  14
• 可旋转键数：
  0
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.0
• 拓扑面积：
  90.5
• 氢给体数：
  2
• 氢受体数：
  4

安全信息

• 危险品标志：
  Xi
• 安全说明：
  S26,S36
• 危险类别码：
  R36/37/38
• WGK Germany：
  3
• 海关编码：
  2933990090
• 危险标志：
  GHS07
• 危险性描述：
  H315,H319,H335
• 危险性防范说明：
  P261,P305 + P351 + P338
• 储存条件：
  保持贮藏器密封，并将其放入一个紧密的容器中。应储存在阴凉、干燥的地方。

SDS

Name:	5-Amino-2 3-dihydro-1 4-phthalazinedione sodium salt Material Safety Data Sheet
Synonym:	Luminol sodium salt; 5-Amino-2,3-dihydro-1,4-phthalazinedione, monosodium salt; 3-Aminophthalic acid hydrazide, sodium salt; Tamerit
CAS:	20666-12-0

Section 1 - Chemical Product MSDS Name:5-Amino-2 3-dihydro-1 4-phthalazinedione sodium salt Material Safety Data Sheet
Synonym:Luminol sodium salt; 5-Amino-2,3-dihydro-1,4-phthalazinedione, monosodium salt; 3-Aminophthalic acid hydrazide, sodium salt; Tamerit

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Section 2 - COMPOSITION, INFORMATION ON INGREDIENTS

CAS#	Chemical Name	content	EINECS#
20666-12-0	5-Amino-2,3-dihydro-1,4-phthalazinedio	100	unlisted

Hazard Symbols: XI
Risk Phrases: 36/37/38

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Section 3 - HAZARDS IDENTIFICATION
EMERGENCY OVERVIEW
Irritating to eyes, respiratory system and skin.
Potential Health Effects
Eye:
Causes eye irritation.
Skin:
Causes skin irritation.
Ingestion:
May cause gastrointestinal irritation with nausea, vomiting and diarrhea.
Inhalation:
Causes respiratory tract irritation. The toxicological properties of this substance have not been fully investigated.
Chronic:
No information found.

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Section 4 - FIRST AID MEASURES
Eyes: In case of contact, immediately flush eyes with plenty of water for at least 15 minutes. Get medical aid.
Skin:
In case of contact, flush skin with plenty of water. Remove contaminated clothing and shoes. Get medical aid if irritation develops and persists. Wash clothing before reuse.
Ingestion:
If swallowed, do not induce vomiting unless directed to do so by medical personnel. Never give anything by mouth to an unconscious person. Get medical aid.
Inhalation:
If inhaled, remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical aid.
Notes to Physician:

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Section 5 - FIRE FIGHTING MEASURES
General Information:
As in any fire, wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH (approved or equivalent), and full protective gear. During a fire, irritating and highly toxic gases may be generated by thermal decomposition or combustion.
Extinguishing Media:
Use water spray, dry chemical, carbon dioxide, or appropriate foam.

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Section 6 - ACCIDENTAL RELEASE MEASURES
General Information: Use proper personal protective equipment as indicated in Section 8.
Spills/Leaks:
Vacuum or sweep up material and place into a suitable disposal container. Clean up spills immediately, observing precautions in the Protective Equipment section. Avoid generating dusty conditions.
Provide ventilation.

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Section 7 - HANDLING and STORAGE
Handling:
Wash thoroughly after handling. Wash hands before eating. Remove contaminated clothing and wash before reuse. Use with adequate ventilation. Minimize dust generation and accumulation. Avoid contact with eyes, skin, and clothing. Avoid breathing dust.
Storage:
Keep container closed when not in use. Store in a cool, dry, well-ventilated area away from incompatible substances.

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Section 8 - EXPOSURE CONTROLS, PERSONAL PROTECTION
Engineering Controls:
Facilities storing or utilizing this material should be equipped with an eyewash facility and a safety shower. Use adequate ventilation to keep airborne concentrations low.
Exposure Limits CAS# 20666-12-0: Personal Protective Equipment Eyes: Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA's eye and face protection regulations in 29 CFR 1910.133 or European Standard EN166.
Skin:
Wear appropriate protective gloves to prevent skin exposure.
Clothing:
Wear appropriate protective clothing to prevent skin exposure.
Respirators:
Follow the OSHA respirator regulations found in 29 CFR 1910.134 or European Standard EN 149. Use a NIOSH/MSHA or European Standard EN 149 approved respirator if exposure limits are exceeded or if irritation or other symptoms are experienced.

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Section 9 - PHYSICAL AND CHEMICAL PROPERTIES

Physical State: Powder
Color: off-white
Odor: None reported.
pH: Not available.
Vapor Pressure: Not available.
Viscosity: Not available.
Boiling Point: Not available.
Freezing/Melting Point: > 300 deg C
Autoignition Temperature: Not applicable.
Flash Point: Not applicable.
Explosion Limits, lower: Not available.
Explosion Limits, upper: Not available.
Decomposition Temperature: Not available.
Solubility in water: Soluble.
Specific Gravity/Density: Not available.
Molecular Formula: C8H6N3O2Na
Molecular Weight: 199.1

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Section 10 - STABILITY AND REACTIVITY
Chemical Stability:
Stable under normal temperatures and pressures.
Conditions to Avoid:
Dust generation, excess heat.
Incompatibilities with Other Materials:
Strong oxidizing agents.
Hazardous Decomposition Products:
Nitrogen oxides, carbon monoxide, carbon dioxide.
Hazardous Polymerization: Has not been reported.

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Section 11 - TOXICOLOGICAL INFORMATION
RTECS#:
CAS# 20666-12-0 unlisted.
LD50/LC50:
Not available.
LD - lethal dose, oral rat >500 mg/kg for Luminol (CAS = 521-31-3).
Carcinogenicity:
5-Amino-2,3-dihydro-1,4-phthalazinedione, sodium salt - Not listed by ACGIH, IARC, or NTP.

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Section 12 - ECOLOGICAL INFORMATION

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Section 13 - DISPOSAL CONSIDERATIONS
Products which are considered hazardous for supply are classified as Special Waste and the disposal of such chemicals is covered by regulations which may vary according to location. Contact a specialist disposal company or the local waste regulator for advice. Empty containers must be decontaminated before returning for recycling.

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Section 14 - TRANSPORT INFORMATION

IATA
Shipping Name: NOT REGULATED
Hazard Class:
UN Number:
Packing Group:
IMO
Shipping Name: NOT REGULATED
Hazard Class:
UN Number:
Packing Group:
RID/ADR
Shipping Name: NOT REGULATED
Hazard Class:
UN Number:
Packing group:

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Section 15 - REGULATORY INFORMATION

European/International Regulations
European Labeling in Accordance with EC Directives
Hazard Symbols: XI
Risk Phrases:
R 36/37/38 Irritating to eyes, respiratory system
and skin.
Safety Phrases:
S 26 In case of contact with eyes, rinse immediately
with plenty of water and seek medical advice.
S 36 Wear suitable protective clothing.
WGK (Water Danger/Protection)
CAS# 20666-12-0: No information available.
Canada
None of the chemicals in this product are listed on the DSL/NDSL list.
CAS# 20666-12-0 is not listed on Canada's Ingredient Disclosure List.
US FEDERAL
TSCA
CAS# 20666-12-0 is not listed on the TSCA inventory.
It is for research and development use only.

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SECTION 16 - ADDITIONAL INFORMATION
N/A

制备方法与用途

RP底物：鲁米诺

鲁米诺（3-氨基邻苯二甲酰肼）是最早使用、应用最广泛且量子产率较高的化学发光试剂之一。自1928年Albrecht首次报道了鲁米诺与氧化剂在碱性溶液中的化学发光行为以来，该化学发光反应主要用于测定氧化剂与无机金属离子。近年来，研究人员不仅进一步研究了这一化学发光反应，还将它与其他多种分析技术结合使用，使得其应用范围不断扩大，并已广泛应用于药物分析、生化分析等多个领域。

生物活性

Luminol钠盐是一种化学发光物质，pKa值分别为6.74和15.1。Luminol钠盐的最佳荧光波长为425 nm，常被用作法医检测血迹的诊断工具，在刑事侦查、生物工程及化学示踪等领域中发挥重要作用。

体外研究

鲁米诺浓度是影响化学发光（CL）强度的重要因素。鲁米诺化学发光（LCL）的强度不仅取决于鲁米诺自身浓度，还与氧化剂浓度、酶浓度以及pH值等其他因素有关。记录到的最大CL强度出现在0.3 mM鲁米诺浓度下。在0.01至0.3 mM范围内，随着鲁米诺浓度增加，CL强度呈线性增长。然而，进一步提高鲁米诺浓度会导致CL强度下降。