5-(氨基甲基)-2'-脱氧尿苷 | 70465-55-3

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘧啶核苷
• 中文名称: 5-(氨基甲基)-2'-脱氧尿苷
• 英文名称: 5'-O-Aminothymidine
• 英文别名: 1-[(2R,4S,5R)-5-(aminooxymethyl)-4-hydroxyoxolan-2-yl]-5-methylpyrimidine-2,4-dione
• CAS: 70465-55-3
• 化学式: C₁₀H₁₅N₃O₅
• 分子量: 257.246
• InChiKey: CHNUETKYLYMAAU-XLPZGREQSA-N

物化性质

• 密度：
  1.499±0.06 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  -1.9
• 重原子数：
  18
• 可旋转键数：
  3
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.6
• 拓扑面积：
  114
• 氢给体数：
  3
• 氢受体数：
  6

反应信息

• 作为产物：
  描述：

  5'-O-phthalimidothymidine 在 甲基肼 作用下， 以 甲醇 、 二氯甲烷 为溶剂， 以77%的产率得到5-(氨基甲基)-2'-脱氧尿苷
  参考文献：
  名称：

  Synthesis of 5'-O-Amino-2'-Deoxypyrimidine and Purine Nucleosides: Building-Blocks for Antisense Oligonucleotides

  摘要：

  An efficient synthesis of 5'-O-amino-2'-deoxy analogs of uridine 1, thymidine 2, cytidine 3, 5-methylcytidine 3a, adenosine 4, and guanosine 5 was accomplished. The key step of 5'-O-N-bond formation in 2'-deoxynucleosides 1-5 was achieved via a Mitsunobu reaction in excellent yields. The 5'-O-amino nucleosides 1-5 are useful building-blocks for the synthesis of nucleoside dimers linked by a methylene(methylimino) (MMI) bridge. MMI is a novel phosphate surrogate for antisense oligonucleosides.

  DOI：

  10.1021/jo00121a037

文献信息

• Methods for detecting aggregations of proteins
  申请人：——

  公开号：US20040197815A1

  公开（公告）日：2004-10-07

  Families of compositions are provided as labels, referred to as eTag reporters for attaching to polymeric compounds and assaying based on release of the eTag reporters from the polymeric compound and separation and detection. For oligonucleotides, the eTag reporters are synthesized at the end of the oligonucleotide by using phosphiste or phosphate chemistry, whereby mass-modifying regions, charge-modifying regions and detectable regions are added sequentially to produce the eTag labeled reporters. By using small building blocks and varying their combination large numbers of different eTag reporters can be readily produced attached to the oligonucleotide of interest for identification. Protocols are used that release the eTag reporter when the target sequence is present in the sample.

  将组成家族作为标签提供，称为eTag报告者，用于附着于聚合物化合物并基于从聚合物化合物释放eTag报告者以及分离和检测进行测定。对于寡核苷酸，eTag报告者通过使用磷酸酯或磷酸化学在寡核苷酸末端合成，逐步添加质量修饰区域、电荷修饰区域和可检测区域，以产生eTag标记的报告者。通过使用小的构建块并变化它们的组合，可以轻松地产生大量不同的eTag报告者，附着于感兴趣的寡核苷酸进行识别。当目标序列存在于样品中时，使用协议释放eTag报告者。
• METHODS AND COMPOSITIONS FOR ENHANCING DETECTION IN DETERMINATIONS EMPLOYING CLEAVABLE ELECTROPHORETIC TAG REAGENTS
  申请人：Aclara BioSciences, Inc.

  公开号：US20040096825A1

  公开（公告）日：2004-05-20

  Probe sets for the multiplexed detection of the binding of, or interaction between, one or more ligands and target antiligands are provided. Detection involves the release of identifying tags as a consequence of target recognition. The probe sets include electrophoretic tag probes or e-tag probes, comprising a detection region and a mobility-defining region called the mobility modifier, both linked to a target-binding moiety. The probes comprise interactive functionalities adjacent the cleaved portion positioned in the probes such that the interactive functionality does not form part of the e-tag reporters. Target antiligands are contacted with a set of e-tag probes and the contacted antiligands are treated with a selected cleaving agent resulting in a mixture of e-tag reporters and uncleaved and/or partially cleaved e-tag probes. The mixture is exposed to a capture agent effective to bind to uncleaved or partially cleaved e-tag probes, followed by electrophoretic separation. In a multiplexed assay, different released e-tag reporters may be separated and detected providing for target identification.

  提供了一种用于多重检测一种或多种配体与目标抗配体之间的结合或相互作用的探针集。检测涉及由于目标识别而释放识别标签。探针集包括电泳标签探针或e标签探针，包括一个检测区域和一个称为迁移修饰剂的迁移定义区域，两者都链接到一个目标结合部位。探针包括与探针中定位的割裂部分相邻的交互功能，使得交互功能不构成e标签报告者的一部分。将目标抗配体与一组e标签探针接触，并用所选的切割剂处理接触的抗配体，从而产生e标签报告者和未割裂和/或部分割裂的e标签探针的混合物。将混合物暴露于有效结合到未割裂或部分割裂的e标签探针的捕获剂后进行电泳分离。在多重检测中，可以分离和检测不同的释放的e标签报告者，从而实现目标识别。
• Sets of generalized target-binding e-tag probes
  申请人：——

  公开号：US20040166529A1

  公开（公告）日：2004-08-26

  Probe sets for the multiplexed detection of the binding of, or interaction between, one or more ligands and target antiligands are provided. Detection involves the release of identifying tags as a consequence of target recognition. The probe sets include electrophoretic tag probes or e-tag probes, comprising a detection region and a mobility-defining region called the mobility modifier, both linked to a target-binding moiety. Target antiligands are contacted with a set of e-tag probes and the contacted antiligands are treated with a selected cleaving agent resulting in a mixture of e-tag reporters and uncleaved and/or partially cleaved e-tag probes. The mixture is exposed to a capture agent effective to bind to uncleaved or partially cleaved e-tag probes, followed by electrophoretic separation. In a multiplexed assay, different released e-tag reporters may be separated and detected providing for target identification.

  提供用于多重检测一种或多种配体与靶抗配体结合或相互作用的探针集。检测涉及识别靶标后标识标签的释放。探针集包括电泳标签探针或e-tag探针，包括检测区域和称为运动修饰剂的运动定义区域，两者都链接到靶标结合基团。将靶抗配体与一组e-tag探针接触，并用选择的切割剂处理接触的抗配体，导致e-tag报告物和未切割和/或部分切割的e-tag探针的混合物。混合物暴露于有效结合到未切割或部分切割的e-tag探针的捕获剂，然后进行电泳分离。在多重检测中，不同释放的e-tag报告物可以分离和检测，从而实现靶标的识别。
• Methods and compositions for enhancing detection in determinations employing cleavable electrophoretic tag reagents
  申请人：Chenna Ahmed

  公开号：US20050053939A1

  公开（公告）日：2005-03-10

  Probe sets for the multiplexed detection of the binding of, or interaction between, one or more ligands and target antiligands are provided. Detection involves the release of identifying tags as a consequence of target recognition. The probe sets include electrophoretic tag probes or e-tag probes, comprising a detection region and a mobility-defining region, both linked to a target-binding moiety. In a multiplexed assay, different released e-tag reporters may be separated and detected providing for target identification. The probes comprise interactive functionalities adjacent the cleaved portion positioned in the probes such that the interactive functionality does not form part of the e-tag reporters. Also described are biopolymers and nucleosides containing such interactive functionalities.

  提供了用于多重检测一个或多个配体与目标抗配体之间结合或相互作用的探针组。检测涉及标识标签的释放，这是目标识别的结果。探针集包括电泳标签探针或e-tag探针，包括检测区域和移动性定义区域，两者都与目标结合基团相连。在多重检测中，不同的释放的e-tag报告者可以分离和检测，从而实现目标识别。探针包括位于探针中定位的割裂部分相邻的交互功能，使交互功能不构成e-tag报告者的一部分。还描述了包含这种交互功能的生物聚合物和核苷酸。
• Tag library compounds, compositions, kits and methods of use
  申请人：——

  公开号：US20040063114A1

  公开（公告）日：2004-04-01

  Families of compositions are provided as labels, referred to as eTag reporters for attaching to polymeric compounds and assaying based on release of the eTag reporters from the polymeric compound and separation and detection. For oligonucleotides, the eTag reporters are synthesized at the end of the oligonucleotide by using phosphite or phosphate chemistry, whereby mass-modifying regions, charge-modifying regions and detectable regions are added sequentially to produce the eTag labeled reporters. By using small building blocks and varying their combination large numbers of different eTag reporters can be readily produced attached to a binding compound specific for the target compound of interest for identification. Protocols are used that release the eTag reporter when the target compound is present in the sample.

  提供组合物家族作为标签，称为eTag报告者，用于附着于聚合物化合物并基于从聚合物化合物中释放eTag报告者以及分离和检测进行测定。对于寡核苷酸，eTag报告者通过使用亚磷酸酯或磷酸酯化学在寡核苷酸末端合成，从而依次添加质量修饰区域、电荷修饰区域和可检测区域，以生成eTag标记的报告者。通过使用小型构建块并改变它们的组合，可以轻松地产生大量不同的eTag报告者，这些报告者附着于与所需目标化合物特异性结合的结合化合物上进行识别。当样品中存在目标化合物时，使用协议释放eTag报告者。