Fmoc-6-aminohexylphosphate | 819074-50-5

• 分子结构分类: 有机化合物 - 苯类化合物 - 芴
• 英文名称: Fmoc-6-aminohexylphosphate
• 英文别名: 9H-fluoren-9-ylmethyl 6-(phosphonooxy)hexylcarbamate；9H-fluoren-9-ylmethyl N-(6-phosphonooxyhexyl)carbamate
• CAS: 819074-50-5
• 化学式: C₂₁H₂₆NO₆P
• 分子量: 419.414
• InChiKey: BOSBWVCCMUXFCF-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  2.7
• 重原子数：
  29
• 可旋转键数：
  11
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.38
• 拓扑面积：
  105
• 氢给体数：
  3
• 氢受体数：
  6

反应信息

• 作为反应物：
  描述：

  Fmoc-6-aminohexylphosphate 在 ammonium hydroxide 作用下， 以 N,N-二甲基甲酰胺 为溶剂， 生成 ammonium 6-aminohexyl hydrogen phosphate
  参考文献：
  名称：

  ω-氨基烷基磷酸氢铵的简便合成

  摘要：

  通过简单有效的三步法合成了一系列的ω-氨基烷基磷酸氢铵。原料ω-氨基烷基醇（AC- n，碳原子数n  = 3、4、5、6 ）用9-芴基甲基氯甲酸酯（Fmoc-Cl）进行氨基保护，然后用POCl 3进行磷酸化并脱保护在哌啶/ DMF中。每种中间体和最终产物的结构均通过1 H NMR，FTIR和质谱确证。每个步骤的产率约为77-92％，总产率高于56％。这种新方法在低成本原料，温和的反应温度（0–25°C）和简便的纯化方法方面具有优势。

  DOI：

  10.1016/j.cclet.2012.06.010
• 作为产物：
  描述：

  6-(Fmoc-氨基)-1-己醇 在 水 、 三氯氧磷 作用下， 以 四氢呋喃 为溶剂， 反应 11.5h， 生成 Fmoc-6-aminohexylphosphate
  参考文献：
  名称：

  ω-氨基烷基磷酸氢铵的简便合成

  摘要：

  通过简单有效的三步法合成了一系列的ω-氨基烷基磷酸氢铵。原料ω-氨基烷基醇（AC- n，碳原子数n  = 3、4、5、6 ）用9-芴基甲基氯甲酸酯（Fmoc-Cl）进行氨基保护，然后用POCl 3进行磷酸化并脱保护在哌啶/ DMF中。每种中间体和最终产物的结构均通过1 H NMR，FTIR和质谱确证。每个步骤的产率约为77-92％，总产率高于56％。这种新方法在低成本原料，温和的反应温度（0–25°C）和简便的纯化方法方面具有优势。

  DOI：

  10.1016/j.cclet.2012.06.010

文献信息

• Novel compositions magnetic particles covered with gem-bisphosphonate derivatives
  申请人：Port Marc

  公开号：US20100297025A1

  公开（公告）日：2010-11-25

  The invention relates to a composition comprising acid magnetic particles (p) based on an iron compound, the acid magnetic particles (p) being complexed by one or more gem-bisphosphonate compounds, of formula I: X-L-CH(PO 3 H 2 ) 2 (I) in which: L represents an organic group connecting the X group to the gem-bisphosphonate group —CH(PO 3 H 2 ) 2 ; X represents a chemical group capable of reacting with a biovector; all or some of the X groups of the particles optionally being coupled to a biovector. The invention relates also to a process for the preparation of the compositions and their use, in particular as contrast products for Magnetic Resonance Imaging (MRI).

  这项发明涉及一种包含基于铁化合物的酸性磁性颗粒（p）的组合物，所述酸性磁性颗粒（p）被一个或多个式I的双膦酸盐化合物络合： X-L-CH(PO3H2)2(I) 其中： L代表将X基团连接到双膦酸盐基团—CH(PO3H2)2的有机基团； X代表能够与生物载体发生反应的化学基团；颗粒的一个或多个X基团可选择地与生物载体偶联。 该发明还涉及一种制备所述组合物的方法及其用途，特别是作为磁共振成像（MRI）对比剂。
• Fluorescent nucleotide analogs and uses therefor
  申请人：Xu Yue

  公开号：US20070072196A1

  公开（公告）日：2007-03-29

  Labeled nucleotide analogs used in place of naturally occurring nucleoside triphosphates or other analogs in template directed nucleic acid synthesis reactions and other nucleic acid reactions, and various analyses based thereon, including DNA sequencing, single base identification, hybridization assays and others.

  在模板定向的核酸合成反应和其他核酸反应以及基于此的各种分析中，使用标记核苷酸模拟物代替自然存在的核苷三磷酸或其他类似物，包括DNA测序、单碱基识别、杂交检测等。
• Tagged multi-nucleotides useful for nucleic acid sequencing
  申请人：Roche Sequencing Solutions, Inc.

  公开号：US10655174B2

  公开（公告）日：2020-05-19

  The present disclosure relates to tagged multi-nucleotide compounds, which comprise a single tag moiety covalently linked to a plurality of nucleoside-5′-oligophosphate moieties. As disclosed herein, these tagged multi-nucleotide compounds have improved characteristics as polymerase substrates and can be used in a range of nucleic acid detection and sequencing methods, including nanopore sequencing-by-synthesis.

  本公开涉及标记的多核苷酸化合物，其包括与多个核苷-5′-寡聚磷酸共价连接的单个标记分子。如本文所公开的，这些标记的多核苷酸化合物作为聚合酶底物具有更好的特性，可用于一系列核酸检测和测序方法，包括纳米孔合成测序。
• Methods and apparatus for single molecule sequencing using energy transfer detection
  申请人：Life Technologies Corporation

  公开号：US11008612B2

  公开（公告）日：2021-05-18

  Provided herein are systems and methods for nucleotide incorporation reactions. The systems comprise polymerases having altered nucleotide incorporation kinetics and are linked to an energy transfer donor moiety, and nucleotide molecules linked with at least one energy transfer acceptor moiety. The donor and acceptor moieties undergo energy transfer when the polymerase and nucleotide are proximal to each other during nucleotide binding and/or nucleotide incorporation. As the donor and acceptor moieties undergo energy transfer, they generate an energy transfer signal which can be associated with nucleotide binding or incorporation. Detecting a time sequence of the generated signals, or the change in the signals, can be used to determine the order of the incorporated nucleotides, and can therefore be used to deduce the sequence of the target molecule.

  本文提供了用于核苷酸掺入反应的系统和方法。这些系统包括具有改变的核苷酸掺入动力学并与能量转移供体分子相连的聚合酶，以及与至少一个能量转移受体分子相连的核苷酸分子。在核苷酸结合和/或核苷酸掺入过程中，当聚合酶和核苷酸彼此靠近时，供体和受体分子会发生能量转移。当供体和受体分子发生能量转移时，它们会产生能量转移信号，该信号可与核苷酸结合或掺入相关联。检测所产生信号的时间序列或信号的变化，可用于确定掺入核苷酸的顺序，从而推断目标分子的序列。
• Long, Processive Enzymatic DNA Synthesis Using 100% Dye-Labeled Terminal Phosphate-Linked Nucleotides
  作者：Jonas Korlach、Arek Bibillo、Jeffrey Wegener、Paul Peluso、Thang T. Pham、Insil Park、Sonya Clark、Geoff A. Otto、Stephen W. Turner

  DOI：10.1080/15257770802260741

  日期：2008.8.28

  We demonstrate the efficient synthesis of DNA with complete replacement of the four deoxyribonucleoside triphosphate (dNTP) substrates with nucleotides carrying fluorescent labels. A different, spectrally separable fluorescent dye suitable for single molecule fluorescence detection was conjugated to each of the four dNTPs via linkage to the terminal phosphate. Using these modified nucleotides, DNA synthesis by phi 29 DNA polymerase was observed to be processive for products thousands of bases in length, with labeled nucleotide affinities and DNA polymerization rates approaching unmodified dNTP levels. Results presented here show the compatibility of these nucleotides for single-molecule, real-time DNA sequencing applications.