5-Hydroxy-2'-deoxycytidine 5'-triphosphate

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘧啶核苷酸
• 英文名称: 5-Hydroxy-2'-deoxycytidine 5'-triphosphate
• 英文别名: [[(2R,3S,5R)-5-(4-amino-5-hydroxy-2-oxopyrimidin-1-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate
• 化学式: C₉H₁₆N₃O₁₄P₃
• 分子量: 483.16
• InChiKey: APWMGTRTGVBXNC-UBKIQSJTSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -5.6
• 重原子数：
  29
• 可旋转键数：
  8
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.56
• 拓扑面积：
  268
• 氢给体数：
  7
• 氢受体数：
  14

反应信息

• 作为产物：
  描述：

  5-Hydroxy-cytidin-5'-diphosphat 、 2'-脱氧胞苷三磷酸 、 钠 、 溴 在 溴 、 环己烯 、 乙醚 、 水 、 triphosphates 、 三乙基碳酸氢铵缓冲液 、 nucleoside triphosphates 、 乙醇 、 碳酸氢铵 作用下， 以 水 为溶剂， 反应 2.0h， 生成 5-Hydroxy-2'-deoxycytidine 5'-triphosphate
  参考文献：
  名称：

  STRONGLY BOUND BASE-MODIFIED OLIGONUCLEOTIDES

  摘要：

  本发明提供了具有螯合基团和寡核苷酸序列的化合物，其中寡核苷酸包括一个或多个修饰的核碱基，例如羟基核碱基。所述化合物适用于反义治疗。螯合基团可以与镧系金属离子形成络合物。这些化合物是核酸的有效翻译抑制剂，并具有增加的靶标核酸结合亲和力。本发明还包括这些化合物的组合物和使用这些组合物作为反义治疗的方法。

  公开号：

  US20120171279A1

文献信息

• Antisense Agents Combining Strongly Bound Base-Modified Oligonucleotide and Artificial Nuclease
  申请人：Karelson Mati

  公开号：US20070259830A1

  公开（公告）日：2007-11-08

  The present invention provides compounds having a chelating moiety and an oligonucleotide sequence wherein the oligonucleotide includes one or more modified nucleobases, such as hydroxynucleobases. The disclosed compounds are suitable for antisense therapy. The chelating moiety can be complexed to an ion of a lanthanide metal. These compounds are efficient translation inhibitors of nucleic acids and have increased binding affinity for target nucleic acids. The invention also includes compositions and methods of using these compositions as antisense therapy.

  本发明提供了具有螯合基团和寡核苷酸序列的化合物，其中寡核苷酸包括一个或多个修饰的核碱基，例如羟基核碱基。所述化合物适用于反义疗法。螯合基团可以与镧系金属离子形成络合物。这些化合物是核酸的高效翻译抑制剂，并且对目标核酸具有增强的结合亲和力。本发明还包括使用这些组合物作为反义疗法的组合物和方法。
• Antisense agents combining strongly bound base-modified oligonucleotide and artificial nuclease
  申请人：Baltic Technology Development, Ltd.

  公开号：US07786292B2

  公开（公告）日：2010-08-31

  The present invention provides compounds having a chelating moiety and an oligonucleotide sequence wherein the oligonucleotide includes one or more modified nucleobases, such as hydroxynucleobases. The disclosed compounds are suitable for antisense therapy. The chelating moiety can be complexed to an ion of a lanthanide metal. These compounds are efficient translation inhibitors of nucleic acids and have increased binding affinity for target nucleic acids. The invention also includes compositions and methods of using these compositions as antisense therapy.

  本发明提供了一种具有螯合基团和寡核苷酸序列的化合物，其中寡核苷酸包括一个或多个修饰的核碱基，如羟基核碱基。所述化合物适用于反义疗法。螯合基团可以与镧系金属离子形成络合物。这些化合物是核酸的高效翻译抑制剂，并具有更高的靶标核酸结合亲和力。本发明还包括使用这些化合物作为反义疗法的组合物和方法。
• Methods of screening for nucleoside analogs that are incorporated by HIV reverse transcriptase and cause incorrect base pairing
  申请人：DARWIN MOLECULAR CORPORATION

  公开号：EP1004675A2

  公开（公告）日：2000-05-31

  Methods and compositions related to HIV are disclosed. Using the methods of the present invention, nucleoside analogs may be screened for the ability to be incorporated by reverse transcriptase of human immunodeficiency virus ("HIV RT") and cause incorrect base pairing. Progressive mutation of the virus by such nucleoside analogs renders it non-viable.

  本发明公开了与艾滋病毒有关的方法和组合物。使用本发明的方法，可以筛选出核苷类似物，以确定其是否能被人类免疫缺陷病毒（"HIV RT"）的逆转录酶结合，并导致不正确的碱基配对。这种核苷类似物可使病毒发生渐进变异，从而使其丧失生存能力。
• Sequencing using non-natural nucleotides
  申请人：Ultima Genomics, Inc.

  公开号：US11326207B2

  公开（公告）日：2022-05-10

  The present disclosure provides methods and systems for nucleic acid sequencing. Such systems and methods may achieve context-independent incorporation, have reduced context-dependence or have context-dependence that is amenable to calibration and modeling. Such systems and methods may also reduce misincorporation.

  本公开提供了核酸测序的方法和系统。这些系统和方法可实现与上下文无关的整合，降低上下文依赖性，或具有可进行校准和建模的上下文依赖性。这些系统和方法还可以减少误结合。
• Partially double-stranded nucleic acids, methods of making, and use thereof
  申请人：——

  公开号：US20020127575A1

  公开（公告）日：2002-09-12

  A process is disclosed for generating at least one partially double-stranded target nucleic acid, which contains at least one single-stranded region at a terminal end. The process comprises the steps of (a) providing at least one primer, P 1 , containing at least one labile nucleotide; (b) combining at least one target nucleic acid sequence with P 1 to generate a double-stranded polynucleotide containing at least one labile nucleotide; (c) exposing the double-stranded polynucleotide to conditions that promote single-strand cleavage of the polynucleotide at the site of the at least one labile nucleotide of primer P 1 ; and (d) exposing the cleaved polynucleotide to conditions that promote the dissociation of the cleaved portions of primer P 1 from a terminal end. The labile nucleotide may be dUTP, wherein the single-stranded cleavage of the polynucleotide at the site of the labile nucleotide occurs by treatment with uracil N-glycosylase.

  本发明公开了一种用于产生至少一种部分双链目标核酸的工艺，该核酸的末端含有至少一个单链区。该工艺包括以下步骤 (a) 提供至少一种引物 P 1 (b) 将至少一个靶核酸序列与 P 1 生成含有至少一个易损核苷酸的双链多核苷酸；(c) 将双链多核苷酸暴露于可促进多核苷酸在引物 P 1 的至少一个易损核苷酸位点处发生单链裂解的条件下。 1 和 (d) 将裂解的多核苷酸暴露于促进引物 P 1 从末端解离的条件。可变核苷酸可以是 dUTP，其中多核苷酸在可变核苷酸位点的单链裂解是通过尿嘧啶 N-糖基化酶处理进行的。