Acetic acid;3',6'-dihydroxyspiro[2-benzofuran-3,9'-xanthene]-1-one

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 异苯并呋喃
• 英文名称: Acetic acid;3',6'-dihydroxyspiro[2-benzofuran-3,9'-xanthene]-1-one
• 化学式: C22H16O7
• 分子量: 392.4
• InChiKey: CPRVUBZYHZGASQ-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  3.76
• 重原子数：
  29
• 可旋转键数：
  0
• 环数：
  5.0
• sp3杂化的碳原子比例：
  0.09
• 拓扑面积：
  113
• 氢给体数：
  3
• 氢受体数：
  7

文献信息

• Promiscuous G-protein compositions and their use
  申请人：Negulescu Paul

  公开号：US20060008855A1

  公开（公告）日：2006-01-12

  Disclosed are compositions and methods for their use, such as in identifying G-protein coupled receptors and ligands and compounds that modulate signal transduction. The compositions and methods employ promicuous G-proteins. Activation of the promiscous G-protein can be detected in a variety of assays, including assays in which activation is indicated by a change in fluorescence emission of a sample that contains the composition.

  揭示了用于识别G蛋白偶联受体、配体以及调节信号转导的化合物的组合物和使用它们的方法。这些组合物和方法利用了多功能G蛋白。可以通过各种测定方法检测多功能G蛋白的激活，包括在激活导致含有该组合物的样品的荧光发射变化的测定中。
• Substrates for beta-lactamase and uses thereof
  申请人：Tsien Y. Roger

  公开号：US20070184513A1

  公开（公告）日：2007-08-09

  Substrates for β-lactamase of the general formula I in which one of X and Y is a fluorescent donor moiety and the other is a quencher (which may or may not re-emit); R′ is selected from the group consisting of H, lower (i.e., alkyl of 1 to about 5 carbon atoms) and (CH 2 ) n OH, in which n is 0 or an integer from 1 to 5; R″ is selected from the group consisting of H, physiologically acceptable metal and ammonium cations, CHR 2 OCO(CH 2 ) n CH 3 , —CHR 2 OCOC (CH 3 ) 3 , acylthiomethyl, acyloxy-alpha -benzyl, delta-butyrolactonyl, methoxycarbonyloxymethyl, phenyl, methylsulphinylmethyl, beta-morpholinoethyl, dialkylaminoethyl, acyloxyalkyl, dialkylaminocarbonyloxymethyl and aliphatic, in which R 2 is selected from the group consisting of H and lower alkyl; A is selected from the group consisting of S, O, SO, SO 2 and CH 2 ; and Z′ and Z″ are linkers for the fluorescent donor and quencher moieties. Methods of assaying β-lactamase activity and monitoring expression in systems using β-lactamase as a reporter gene also are disclosed.

  通式I的β-内酰胺酶底物，其中X和Y中的一个是荧光给体基团，另一个是猝灭剂（可以或不可以再发射）；R'从H，较低的（即1到约5个碳原子的烷基）和（CH2）nOH中选择，其中n为0或1到5的整数；R"从H，生理上可接受的金属和铵阳离子，CHR2OCO（CH2）nCH3，—CHR2OCOC（CH3）3，酰硫甲基，酰氧-α-苯甲基，δ-丁酰内酯，甲氧羰氧甲基，苯基，甲硫氧甲基，β-吗啡啉乙基，二烷基氨基乙基，酰氧烷基，二烷基氨基羰氧甲基和脂肪族中选择，其中R2从H和较低的烷基中选择；A从S，O，SO，SO2和CH2中选择；Z'和Z"是荧光给体和猝灭剂基团的连接器。还公开了评估β-内酰胺酶活性和使用β-内酰胺酶作为报告基因监测表达的方法。
• Method for detection of peritoneal inflammation or infection
  申请人：ABBOTT LABORATORIES

  公开号：EP0178501A1

  公开（公告）日：1986-04-23

  Peritoneal inflammation or infection can be detected in a patient by assaying a peritoneal lavage sample from the patient for lysosomal enzymes. This is done by combining the lavage sample with a leukocyte lysing agent and a chromogenic or fluorogenic enzyme substrate specific for lysosomat enzymes. The lysosomal enzymes can then be measured, and the inflammation or infection detected, by measuring the color or fluorescence developed in the sample by action of the enzymes of the substrate.

  腹膜炎症或感染可通过化验患者腹腔灌洗液样本中的溶酶体酶来检测。具体做法是将灌洗液样本与白细胞裂解剂和溶酶体酶特异性显色或荧光酶底物结合。然后，通过测量底物中酶的作用在样本中形成的颜色或荧光，就可以测量溶酶体酶，并检测炎症或感染。
• Methods for separating malignant cells from clinical specimens
  申请人：ANALYTICAL BIOSYSTEMS CORPORATION

  公开号：EP0277837A2

  公开（公告）日：1988-08-10

  Fragments of a biopsy sample on the order of about 50 to 5000 cells are preferred for establishing viable tumor cell cultures for purposes such as establishing cell lines, chemotherapeutic assays and the like. Such fragments retain the three-dimensional cellular structure or organization of the original tumor and, therefore, can be cultured more readily. To obtain such fragments suitable for culturing, the biopsy sample can be enzymatically digested in a proteolytic or nucleolytic enzyme, such as collagenase, or by mechanical dissociation, or both where necessary. The fragments can then be suspended in an aqueous medium so that non-aggregated cells (e.g., red blood cells, lymphocytes, macrophages) and cellular debris will form a supernatant while the remaining fragments containing aggregated tumor cells are deposited in a sediment layer. Preferably, the medium is an isotonic tissue culture medium and decantation is conducted at least twice; first in a serum-containing medium and then, secondly, in a serum-free medium. Fragments containing living tumor cells can be selected by fluorochromasia, that is, by contacting the sedimented layer with a fluorogenic substrate such that viable tumor cells take up and hydrolyse the substrate, and then exhibit fluorescence. Cytotoxicity assay protocols employing tumor cell aggregates prepared by the present techniques are also disclosed.

  活检样本中细胞数量在 50 至 5000 个之间的片段更适合用于建立有活力的肿瘤细胞培养物，如建立细胞系、化疗试验等。这种片段保留了原始肿瘤的三维细胞结构或组织，因此更易于培养。为获得适于培养的此类片段，活检样本可在蛋白水解酶或核酸水解酶（如胶原酶）中进行酶解，或通过机械解离，必要时也可同时进行酶解和机械解离。然后将碎片悬浮在水性培养基中，使非聚集细胞（如红细胞、淋巴细胞、巨噬细胞）和细胞碎片形成上清液，而含有聚集肿瘤细胞的剩余碎片沉积在沉淀层中。培养基最好是等渗组织培养基，倾析至少进行两次；第一次在含血清培养基中，第二次在无血清培养基中。含活肿瘤细胞的片段可通过荧光染色法选出，即用含氟底物接触沉淀层，使有活力的肿瘤细胞吸收并水解底物，然后发出荧光。此外，还公开了采用本技术制备的肿瘤细胞聚集体的细胞毒性检测方案。
• Dry deposition of materials for microarrays using matrix displacement
  申请人：——

  公开号：US20010012537A1

  公开（公告）日：2001-08-09

  A method is disclosed which relates to the placement of binding partners on microarrays, where such binding partners contain proteins, nucleic acids, biological cells and other bio-reactive components. The present invention discloses uses and methods for manufacture of microarrays constructed in part by sectioning bundles of tubules or rods containing matrix immobilized bio-reactive molecules to produce large numbers of sample chips. The chips so produced are processed by deposition to microarrays. The deposited chips can then be manipulated to partition the immobilizing matrix away from the bio-reactive molecules contained in the matrix and to place said partitioned molecules onto various surfaces for subsequent analysis, to include binding assays, hybridization reactions, diagnostic methods and a variety of cell interaction-determining methodologies.

  本发明公开了一种方法，涉及在微阵列上放置结合伙伴，这些结合伙伴包含蛋白质、核酸、生物细胞和其他生物反应成分。本发明公开了微阵列的用途和制造方法，微阵列的部分构造是将含有基质固定的生物反应分子的管束或棒切片，以生产大量的样品芯片。这样生产出来的芯片通过沉积加工成微阵列。然后，可对沉积的芯片进行操作，将固定基质与基质中的生物反应分子分开，并将所述分开的分子放置到各种表面上进行后续分析，包括结合测定、杂交反应、诊断方法和各种细胞相互作用测定方法。