1-Nitro-9,10-anthraquinone-2-carboxylic acid reacts with 2-aminoethanol to give 1-(2-hydroxyethylamino)-9, 10-anthraquinone-2-carboxylic acid which undergoes intramolecular cyclization to 1,2,3,5,8,13-hexahydroanthra[1,2-e][1,4]oxazepine-5,8,13-trione on heating in acetic acid. Reactions of the cyclization product with amines result in cleavage of the seven-membered heteroring.
1-Nitro-9,10-anthraquinone-2-carboxylic acid reacts with 2-aminoethanol to give 1-(2-hydroxyethylamino)-9, 10-anthraquinone-2-carboxylic acid which undergoes intramolecular cyclization to 1,2,3,5,8,13-hexahydroanthra[1,2-e][1,4]oxazepine-5,8,13-trione on heating in acetic acid. Reactions of the cyclization product with amines result in cleavage of the seven-membered heteroring.
The present invention relates to methods for screening, identifying, and/or quantifying modulators of amyloid and/or aggregates, fibrils or components thereof, in particular modulators of amyloid β-peptide (Aβ) or Aβ fibrils. Aspects of the invention provide methods for screening putative modulators against an Amyloid target, in particular an Aβ target, so as to determine which modulators bind to or interact with the target, or interfere with the interaction of an indicator agent and the target. Particular aspects of the invention employ mass spectrometric methods for the screening of an Amyloid target against compound libraries, in particular mixtures of compounds or combinatorial libraries.