2-[5-[1-[6-[2-(2,5-Dioxopyrrol-1-yl)ethylamino]-6-oxohexyl]-3,3-dimethylindol-2-ylidene]penta-1,3-dienyl]-1,3,3-trimethylindol-1-ium-5-sulfonic acid

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 吲哚及其衍生物
• 英文名称: 2-[5-[1-[6-[2-(2,5-Dioxopyrrol-1-yl)ethylamino]-6-oxohexyl]-3,3-dimethylindol-2-ylidene]penta-1,3-dienyl]-1,3,3-trimethylindol-1-ium-5-sulfonic acid
• 英文别名: 2-[5-[1-[6-[2-(2,5-dioxopyrrol-1-yl)ethylamino]-6-oxohexyl]-3,3-dimethylindol-2-ylidene]penta-1,3-dienyl]-1,3,3-trimethylindol-1-ium-5-sulfonic acid
• 化学式: C38H45N4O6S+
• 分子量: 685.9
• InChiKey: FAKZUFOWWUSQAZ-UHFFFAOYSA-O

计算性质

• 辛醇/水分配系数(LogP)：
  4.6
• 重原子数：
  49
• 可旋转键数：
  13
• 环数：
  5.0
• sp3杂化的碳原子比例：
  0.37
• 拓扑面积：
  136
• 氢给体数：
  2
• 氢受体数：
  7

文献信息

• Reagents and a method for saturation labelling of proteins
  申请人：Williams Karen

  公开号：US20050233462A1

  公开（公告）日：2005-10-20

  A matched set of fluorescent dyes is provided, wherein each dye of the set is capable of covalent attachment to a protein and wherein each of the dyes has a molecular structure and a charge that is matched one with the other, such that relative electrophoretic mobility of a protein labelled with one dye of the set is the same as the electrophoretic mobility of the protein labelled with a different dye of the set. The matched set comprises at least two different fluorescent dyes of formula: wherein n is 1, 2, or 3; Z 1 and Z 2 independently represent the carbon atoms necessary to complete a phenyl or naphthyl ring system; one of groups R 1 and R 2 is a target bonding group; remaining group R 1 or R 2 is selected from —(CH 2 ) 4 —W or —(CH 2 ) r —H; group R 3 is hydrogen, except when either R 1 or R 2 is —(CH 2 ) r —H, in which case R 3 is W; and W is selected from sulphonic acid and sulphonate. The invention also provides a method for saturation labelling of a protein with a fluorescent dye so as to label all available target amino acid, suitably cysteine, residues in the protein, thereby giving a single population of labelled protein molecules.

  提供了一组匹配的荧光染料，其中该组染料中的每种染料都能与蛋白质共价连接，每种染料的分子结构和电荷彼此匹配，从而使用该组染料中的一种染料标记的蛋白质的相对电泳迁移率与用该组染料中的另一种染料标记的蛋白质的电泳迁移率相同。匹配的染料组至少包括两种不同的荧光染料，其式如下 其中 n 为 1、2 或 3； Z 1 和 Z 2 分别代表完成苯基或萘基环状系统所需的碳原子；基团 R 1 和 R 2 中的一个是靶键基团；其余基团 R 1 或 R 2 选自-(CH 2 ) 4 -W或-(CH 2 ) r -H；基团 R 3 是氢，除非当 R 1 或 R 2 是-（CH 2 ) r -H，在这种情况下，R 3 是 W；W 选自磺酸和磺酸盐。本发明还提供了一种用荧光染料对蛋白质进行饱和标记的方法，以标记蛋白质中所有可用的目标氨基酸残基，最好是半胱氨酸残基，从而得到单一的标记蛋白质分子群。
• REAGENTS AND A METHOD FOR SATURATION LABELLING OF PROTEINS
  申请人：GE Healthcare UK Limited

  公开号：EP1520176B1

  公开（公告）日：2010-03-10
• Differential analysis of cell surface proteins on closed membrane structures by labelling with dyes in the presence of an internal standard
  申请人：Copse Catherine

  公开号：US20070161116A1

  公开（公告）日：2007-07-12

  Disclosed are matched fluorescent reagents and a method for reproducibly labelling membrane components, such as those expressed on the cell surface, and subsequent differential analysis of the labelled components to detect differences between cell types and states. Furthermore, the present method utilises an internal standard in order to match protein patterns across gels thereby avoiding gel-to-gel variation. The method according to the invention is particularly useful, for example, for detecting low abundance membrane proteins, for detecting changes in receptors expressed in the cell membrane, for example on ligand binding, or in response to stimuli.
• US7511155B2
  申请人：——

  公开号：US7511155B2

  公开（公告）日：2009-03-31