6-Isothiocyanatohexansaeurechlorid | 39243-52-2

• 分子结构分类: 有机化合物 - 有机硫化合物 - 异硫氰酸盐
• 英文名称: 6-Isothiocyanatohexansaeurechlorid
• 英文别名: ε-Isothiocyanatocapronsaeurechlorid；6-isothiocyano-hexanoyl chloride；6-Isothiocyanatohexanoyl chloride
• CAS: 39243-52-2
• 化学式: C₇H₁₀ClNOS
• 分子量: 191.681
• InChiKey: PDLSNXJMSMGTBM-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  3.1
• 重原子数：
  11
• 可旋转键数：
  6
• 环数：
  0.0
• sp3杂化的碳原子比例：
  0.71
• 拓扑面积：
  61.5
• 氢给体数：
  0
• 氢受体数：
  3

反应信息

• 作为反应物：
  描述：

  三甲基氯硅烷 、 6-Isothiocyanatohexansaeurechlorid 生成 6-Isothiocyanatothiohexansaeure-O-trimethylsilylester
  参考文献：
  名称：

  Synthese von Thiocarbonsäure-trimethylsilylestern

  摘要：

  DOI：

  10.1055/s-1971-21817
• 作为产物：
  描述：

  6-Isothiocyanato-hexansaeure 在 氯化亚砜 、 三乙胺 作用下， 以 氯仿 、 乙酸乙酯 为溶剂， 生成 6-Isothiocyanatohexansaeurechlorid
  参考文献：
  名称：

  Kricheldorf,H.R., Justus Liebigs Annalen der Chemie, 1971, vol. 748, p. 101 - 108

  摘要：

  DOI：

文献信息

• Labeled analytes
  申请人：Baker Instruments Corporation

  公开号：US04785080A1

  公开（公告）日：1988-11-15

  A highly sensitive, immunoassay method for determining the amount of an analyte in a sample containing a known analyte in an unknown concentration is provided. Sample; a polypeptide-labeled analog of the analyte, an antibody specific for said analyte, a polypeptide partner capable of non-covalently binding with the polypeptide-labeled analyte to form a complex having catalytic activity, and a substrate capable of being converted to a reporter molecule by the catalytic activity of said complex are brought together in a medium. The polypeptide-labeled analyte analog is capable of competitively binding to the antibody and the polypeptide partner, the antibody inhibiting the formation of a catalytically active complex in the absence of analyte, and the concentration of the antibody, polypeptide partner and polypeptide-labeled analyte are such as to cause varying amounts of analyte to be directly related to the conversion of the substrate to the reporter molecule. Conversion of the substrate to the reporter molecule is then determined, and compared to conversions of substrate to reporter molecule obtained with known concentrations of the analyte.

  本发明提供了一种高灵敏度的免疫分析方法，用于确定含有未知浓度的已知分析物的样品中分析物的数量。将样品、分析物的多肽标记类似物、特异性抗体、能够非共价结合多肽标记的分析物形成具有催化活性的复合物的多肽伙伴以及能够通过复合物的催化活性将底物转化为报告分子的底物在介质中混合。多肽标记的分析物类似物能够与抗体和多肽伙伴竞争性结合，抗体在没有分析物的情况下抑制催化活性复合物的形成，而抗体、多肽伙伴和多肽标记的分析物的浓度使得不同量的分析物与底物转化为报告分子直接相关。然后确定底物到报告分子的转化，并将其与已知浓度的分析物所得到的底物到报告分子的转化进行比较。
• Method for carrying out non-isotopic immunoassays, labeled analytes and
  申请人：Baker Instruments Corporation

  公开号：US04378428A1

  公开（公告）日：1983-03-29

  A highly sensitive, immunoassay method for determining the amount of an analyte in a sample containing a known analyte in an unknown concentration is provided. Sample; a polypeptide-labeled analog of the analyte, an antibody specific for said analyte, a polypeptide partner capable of non-covalently binding with the polypeptide-labeled analyte to form a complex having catalytic activity, and a substrate capable of being converted to a reporter molecule by the catalytic activity of said complex are brought together in a medium. The polypeptide-labeled analyte analog is capable of competitively binding to the antibody and the polypeptide partner, the antibody inhibiting the formation of a catalytically active complex in the absence of analyte, and the concentrations of the antibody, polypeptide partner and polypeptide-labeled analyte are such as to cause varying amounts of analyte to be directly related to the conversion of the substrate to the reporter molecule. Conversion of the substrate to the reporter molecule is then determined, and compared to conversions of substrate to reporter molecule obtained with known concentrations of the analyte.

  提供了一种高灵敏度的免疫分析方法，用于确定样品中含有未知浓度的已知分析物的数量。将样品，包括：分别标记有多肽的分析物类似物、特异性抗体、能够与分别标记有多肽的分析物类似物非共价结合以形成具有催化活性的复合物的多肽伴侣，以及能够通过该复合物的催化活性将底物转化为报告分子的底物，放置在介质中。分别标记有多肽的分析物类似物能够竞争性地结合到抗体和多肽伴侣上，抗体在没有分析物的情况下抑制催化活性复合物的形成，抗体、多肽伴侣和分别标记有多肽的分析物的浓度使得分析物的不同浓度与底物转化为报告分子的量直接相关。然后确定底物转化为报告分子的转化率，并将其与已知分析物浓度下的底物转化为报告分子的转化率进行比较。
• Polypeptide-labeled analyte analog for carrying out an immunoassay
  申请人：Biopharma S.A.

  公开号：US05106950A1

  公开（公告）日：1992-04-21

  A polypeptide-labeled analyte analog for use in an immunoassay is prepared which is capable of binding with a polypeptide partner to provide enzymatic activity such as a ribonuclease or .beta.-galactosidase activity. The polypeptide analog provides a highly sensitive, immunoassay method for determining the amount of an analyte in a sample containing a known analyte in an unknown concentration. To carry out an immunoassay, there is brought together in a medium a sample, the polypeptide-labeled analog of the analyte, an antibody specific for said analyte, a polypeptide partner capable of non-covalently binding with the polypeptide-labeled analyte analog to form a complex having catalytic activity, and a substrate capable of being converted to a reporter molecule by the catalytic activity of said complex. The polypeptide-labeled analyte analog is capable of competitively binding to the antibody and the polypeptide partner, the antibody inhibiting the formation of a catalytically active complex in the absence of analyte, and the concentrations of the antibody, polypeptide partner and polypeptide-labeled analyte are such as to cause varying amounts of analyte to be directly related to the conversion of the substrate to the reporter molecule. Conversion of the substrate to the reporter molecule is then determined, and compared to conversions of substrate to reporter molecule obtained with known concentrations of the analyte.

  制备了一种多肽标记的分析物类似物，用于免疫测定，能够与多肽配体结合形成酶活性，如核酸酶或β-半乳糖苷酶活性。该多肽类似物提供了一种高灵敏度的免疫测定方法，用于确定含有未知浓度的已知分析物的样品中分析物的数量。为了进行免疫测定，在介质中将样品、分析物的多肽标记类似物、特异性针对该分析物的抗体、能够非共价结合多肽标记分析物类似物以形成具有催化活性的复合物的多肽配体以及能够被复合物的催化活性转化为报告分子的底物结合在一起。多肽标记的分析物类似物能够与抗体和多肽配体竞争性结合，抗体在缺乏分析物的情况下抑制催化活性复合物的形成，抗体、多肽配体和多肽标记的分析物的浓度使得不同量的分析物直接相关于底物转化为报告分子的程度。然后确定底物转化为报告分子的程度，并将其与已知分析物浓度下获得的底物转化为报告分子的程度进行比较。
• A method for carrying out non-isotopic immunoassays, labeled analytes and kits for use in such assays
  申请人：Baker Instruments Corporation

  公开号：EP0062277A1

  公开（公告）日：1982-10-13

  A highly sensitive, immunoassay method for determining the amount of an analyte in a sample containing a known analyte in an unknown concentration is provided. Said sample, a polypeptide-labeled analog of the analyte, an antibody specific for said analyte, a polypeptide partner capable of non-covalently binding with the polypeptide-labeled analyte to form a complex having catalytic activity, and a substrate capable of being converted to a reporter molecule by the catalytic activity of said complex are brought together in a medium. The polypeptide-labeled analyte analog is capable of competitively binding to the antibody and the polypeptide partner, the antibody inhibiting the formation of a catalytically active complex in the absence of analyte, and the concentrations of the antibody, polypeptide partner and polypeptide-labeled analyte are such as to cause varying amounts of analyte, to be directly related to the conversion of the substrate to the reporter molecule. Conversion of the substrate to the reporter molecule is then determined, and compared to conversions of substrate to reporter molecule obtained with known concentrations of the analyte.

  本发明提供了一种高灵敏度的免疫测定方法，用于测定含有未知浓度已知分析物的样品中分析物的含量。将所述样品、多肽标记的分析物类似物、针对所述分析物的特异性抗体、能够与多肽标记的分析物非共价结合形成具有催化活性的复合物的多肽伙伴，以及能够在所述复合物的催化活性作用下转化为报告分子的底物一起置于培养基中。多肽标记的分析物类似物能够竞争性地与抗体和多肽伙伴结合，抗体在没有分析物的情况下抑制具有催化活性的复合物的形成，抗体、多肽伙伴和多肽标记的分析物的浓度使分析物的量变化与底物向报告分子的转化直接相关。然后测定底物到报告分子的转化率，并与已知浓度的分析物得到的底物到报告分子的转化率进行比较。
• Kricheldorf,H.R., Angewandte Chemie, 1975, vol. 87, p. 517
  作者：Kricheldorf,H.R.

  DOI：——

  日期：——