4-(3-(1-hydroxyethyl)-4-nitrophenoxy)butanoic acid | 1331954-73-4

• 分子结构分类: 有机化合物 - 苯类化合物 - 苯和取代衍生物
• 英文名称: 4-(3-(1-hydroxyethyl)-4-nitrophenoxy)butanoic acid
• 英文别名: 4-[3-(1-Hydroxyethyl)-4-nitrophenoxy]butanoic acid；4-[3-(1-hydroxyethyl)-4-nitrophenoxy]butanoic acid
• CAS: 1331954-73-4
• 化学式: C₁₂H₁₅NO₆
• 分子量: 269.254
• InChiKey: QQHLVJFTVRPPGT-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  1.1
• 重原子数：
  19
• 可旋转键数：
  6
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.42
• 拓扑面积：
  113
• 氢给体数：
  2
• 氢受体数：
  6

反应信息

• 作为反应物：
  描述：

  4-(3-(1-hydroxyethyl)-4-nitrophenoxy)butanoic acid 在 三溴化磷 作用下， 以 二氯甲烷 为溶剂， 反应 3.5h， 生成 4-(3-(1-bromoethyl)-4-nitrophenoxy)butanoic acid
  参考文献：
  名称：

  [EN] O-NITROBENZYL PHOTOCLEAVABLE BIFUNCTIONAL LINKER
  [FR] LIEUR BIFONCTIONNEL PHOTOCLIVABLE O-NITROBENZYLE

  摘要：

  本文公开了一种邻硝基苄基化合物，或其立体异构体或盐，以及包含该化合物的组合物，并公开了制备该化合物的方法。本文还公开了一种使用该邻硝基苄基化合物或其立体异构体在探针中的方法，该探针能够杂交到、检测或测序核酸分子。

  公开号：

  WO2019104070A1
• 作为产物：
  描述：

  3-羟基苯乙酮 在 sodium tetrahydroborate 、 乙醇 、 硫酸 、 水 、 硝酸 、 potassium carbonate 、 三乙胺 、 三氟乙酸 作用下， 以 乙酸乙酯 、 N,N-二甲基甲酰胺 为溶剂， 反应 2.5h， 生成 4-(3-(1-hydroxyethyl)-4-nitrophenoxy)butanoic acid
  参考文献：
  名称：

  [EN] O-NITROBENZYL PHOTOCLEAVABLE BIFUNCTIONAL LINKER
  [FR] LIEUR BIFONCTIONNEL PHOTOCLIVABLE O-NITROBENZYLE

  摘要：

  本文公开了一种邻硝基苄基化合物，或其立体异构体或盐，以及包含该化合物的组合物，并公开了制备该化合物的方法。本文还公开了一种使用该邻硝基苄基化合物或其立体异构体在探针中的方法，该探针能够杂交到、检测或测序核酸分子。

  公开号：

  WO2019104070A1

文献信息

• Photodegradable Macromers and Hydrogels for Live Cell Encapsulation and Release
  作者：Donald R. Griffin、Andrea M. Kasko

  DOI：10.1021/ja305280w

  日期：2012.8.8

  Hydrogel scaffolds are commonly used as 3D carriers for cells because their properties can be tailored to match natural extracellular matrix. Hydrogels may be used in tissue engineering and regenerative medicine to deliver therapeutic cells to injured or diseased tissue through controlled degradation. Hydrolysis and enzymolysis are the two most common mechanisms employed for hydrogel degradation, but neither allows sequential or staged release of cells. In contrast, photodegradation allows external real-time spatial and temporal control over hydrogel degradation, and allows for staged and sequential release of cells. We synthesized and characterized a series of macromers incorporating photodegradbale ortho-nitrobenzyl (o-NB) groups in the macromer backbone. We formed hydrogels from these macromers via redox polymerization and quantified the apparent rate constants of degradation (k(app)) of each via photorheology at 370 nm, 10 mW/cm(2). Decreasing the number of aryl ethers on the o-NB group increases k(app), and changing the functionality from primary to seconday at the benzylic site dramatically increases k(app). Human mesenchymal stem cells (hMSCs) survive encapsulation in the hydrogels (90% viability postencapsulation). By exploiting the differences in reactivity of two different o-NB linkers, we quantitatively demonstrate the biased release of one stem cell population (green-fluoroescent protein expressing hMSCs) over another (red-fluorescent protein expressing hMSCs).
• SMALL MOLECULE SCREENING CELLULAR ASSAY USING MODIFIED BEADS
  申请人：HOFFMANN-LA ROCHE INC.

  公开号：US20220213471A1

  公开（公告）日：2022-07-07

  A method for screening a DNA-encoded library of chemical structures ( 2 ) for activity in a cellular target ( 11 ) wherein the chemical structures ( 2 ) of the library, the corresponding encoding DNA ( 4 ) and, optionally, a chemical probe ( 7/8/9 ) susceptible to the response molecule ( 12 ) are covalently linked to beads ( 1 ); the method comprising providing an incubation medium ( 13 ) or aliquot thereof comprising the cellular target ( 11 ) and exactly one or more than one bead ( 1 ) as defined above, releasing the chemical structures ( 2 ) from the bead(s) ( 1 ) in the incubation medium ( 13 ) or aliquots thereof by cleaving the structure linkers ( 3 ) and incubating the released chemical structures ( 2 ) and the cellular target ( 11 ); and sequencing the encoding DNA present or remaining on the bead(s) ( 1 ). A bead ( 1 ) suited for the method is also provided.