(2,4-dichloro-benzoyl)-dithiocarbamic acid methyl ester | 98588-82-0

• 分子结构分类: 有机化合物 - 苯类化合物 - 苯和取代衍生物
• 英文名称: (2,4-dichloro-benzoyl)-dithiocarbamic acid methyl ester
• 英文别名: N-<2.4-Dichlor-benzoyl>-dithiocarbaminsaeuremethylester；(2,4-Dichlor-benzoyl)-dithiocarbamidsaeure-methylester
• CAS: 98588-82-0
• 化学式: C₉H₇Cl₂NOS₂
• 分子量: 280.199
• InChiKey: PFTYFVXGBDPLFD-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  3.37
• 重原子数：
  15.0
• 可旋转键数：
  1.0
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.11
• 拓扑面积：
  29.1
• 氢给体数：
  1.0
• 氢受体数：
  3.0

反应信息

• 作为反应物：
  描述：

  (2,4-dichloro-benzoyl)-dithiocarbamic acid methyl ester 在 tetraphosphorus decasulfide 作用下， 以 xylene 为溶剂， 生成 3-Thioxo-5-<2.4-dichlor-phenyl>-3H-<1.2.4>dithiazol
  参考文献：
  名称：

  Derocque,J.-L.; Vialle,J., Bulletin de la Societe Chimique de France, 1966, p. 1183 - 1186

  摘要：

  DOI：
• 作为产物：
  描述：

  甲硫醇 、 potassium thioacyanate 、 2,4-二氯苯甲酰氯 在 乙腈 作用下， 生成 (2,4-dichloro-benzoyl)-dithiocarbamic acid methyl ester
  参考文献：
  名称：

  Elmore et al., Journal of the Chemical Society, 1956, p. 4458,4462

  摘要：

  DOI：

文献信息

• 639. Degradative studies of peptides and proteins. Part V. The formation of 2-acylimino-3-methylthiazolid-5-ones from N-acylthio-carbamoylsarcosine derivatives and their behaviour towards nucleophilic reagents
  作者：D. T. Elmore

  DOI：10.1039/jr9590003152

  日期：——
• Toward Efficient Analysis of Mutations in Single Cells from Ethanol-Fixed, Paraffin-Embedded, and Immunohistochemically Stained Tissues
  作者：Ernst Heinmöller、Qiang Liu、Yuan Sun、Gudrun Schlake、Kathleen A Hill、Larry M Weiss、Steve S Sommer

  DOI：10.1038/labinvest.3780437

  日期：2002.4

  only a few studies have demonstrated successful molecular analysis after whole genome amplification using single cells dissected from paraffin-embedded tissues. The results in these studies were limited by low-amplification efficiency and high rates of allele dropout. In the present study, the amplification rate using a thoroughly modified primer extension and preamplification-PCR protocol was improved significantly for single cells microdissected from paraffin-embedded and immunohistochemically stained tissues. Tissue fixation with ethanol (85%) and the addition of 0.2 mmol/L EDTA helped to achieve an amplification rate between 67% (segments 200 to 400 bp) and 72% (segments <200 bp). Normal tissue sections were immunohistochemically double stained for overabundance of p53 protein and proliferating cell nuclear antigen. Microdissection of single cells was performed with a manual micromanipulator equipped with a Tungsten needle. Sequence analysis of the TP53 gene was performed after improved primer extension preamplification-PCR and multiplex PCR from single microdissected cells. The rate of allele dropout was at least 68%. These technical advances facilitate routine mutation analysis using a single cell or a few cells microdissected from routinely processed paraffin-embedded normal and tumor tissues. Allele dropout still represents a serious problem in single-cell mutation analysis, especially in samples with limited template DNA and prone to DNA damage.