3-Phenylcyclohexa-3,5-diene-1,2-diol | 34244-66-1

• 分子结构分类: 有机化合物 - 苯类化合物 - 苯和取代衍生物
• 英文名称: 3-Phenylcyclohexa-3,5-diene-1,2-diol
• CAS: 34244-66-1
• 化学式: C₁₂H₁₂O₂
• 分子量: 188.226
• InChiKey: UMAHGMFKBJHGME-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  1.1
• 重原子数：
  14
• 可旋转键数：
  1
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.17
• 拓扑面积：
  40.5
• 氢给体数：
  2
• 氢受体数：
  2

反应信息

• 作为反应物：
  描述：

  3-Phenylcyclohexa-3,5-diene-1,2-diol 在 palladium on activated charcoal 、 氢气 作用下， 以 乙酸乙酯 为溶剂， 反应 4.0h， 以99%的产率得到(1S,2R)-3-phenylcyclohex-3-ene-1,2-diol
  参考文献：
  名称：

  氮亲核试剂与对映体纯环己烯基亲电试剂的反应：立体和区域选择性研究

  摘要：

  考察了对映体纯的环己烯环氧化物和反式1,2-溴乙酸酯的反应，它们衍生自相应的取代苯顺式-二氢二醇代谢物，并与氮亲核试剂反应，并提出了可能的机理。最初的目标是合成新的1,2-氨基醇对映异构体，作为潜在的手性配体和合成支架，以产生文库。这些看似简单的取代反应被证明比最初预期的要复杂，并且被发现涉及不同反应机理的组合。

  DOI：

  10.1002/poc.3183

文献信息

• Novel polypeptides and methods for their use
  申请人：——

  公开号：US20030022335A1

  公开（公告）日：2003-01-30

  The invention provides an NDO or NDO related complex comprising at least one alpha-subunit polypeptide that comprises: 1) a substituted amino acid at the position corresponding to position 352 in NDO, 2) a substituted amino acid at the position corresponding to position 201, 202, 260, 316, 351, 358, 362, or 366 in NDO, or 3) a substituted amino acid at the position corresponding to position 352 in NDO, and a substituted amino acid at the position corresponding to position 201, 202, 260, 316, 351, 358, 362, or 366 in NDO; or a catalytically active fragment thereof. The invention also provides DNA encoding such polypeptides, host cells augmented by such DNA, and methods for using the enzymes or host cells to provide useful and novel synthons. The invention also provides novel compounds prepared with the complexes or methods of the invention.

  本发明提供了一种包含至少一个α-亚基多肽的NDO或NDO相关复合物，其中该α-亚基多肽包括：1）在与NDO的352位相对应的位置上的取代氨基酸，2）在与NDO的201、202、260、316、351、358、362或366位相对应的位置上的取代氨基酸，或3）在与NDO的352位相对应的位置上的取代氨基酸和在与NDO的201、202、260、316、351、358、362或366位相对应的位置上的取代氨基酸；或其催化活性片段。本发明还提供了编码这种多肽的DNA，通过这种DNA增强的宿主细胞以及使用这些酶或宿主细胞提供有用和新颖合成物的方法。本发明还提供了使用该复合物或本发明的方法制备的新型化合物。
• Biochemical process
  申请人：IMPERIAL CHEMICAL INDUSTRIES PLC

  公开号：EP0076606A1

  公开（公告）日：1983-04-13

  The production of 1,2-dihydroxy-cyclohexadienes from aromatic compounds by a biochemical process by using strains of Pseudomonas putida. and novel 1,2-disubstituted- cyclo-hexadienes are described. Certain of the novel compounds are useful as intermediates in the production of polymers.

  介绍了利用假单胞菌（Pseudomonas putida）菌株通过生化工艺从芳香族化合物中生产 1,2-二羟基-环己二烯和新型 1,2-二取代-环己二烯的过程。 其中某些新型化合物可用作生产聚合物的中间体。
• Probe compound for detecting and isolating enzymes and means and methods using the same
  申请人：Helmholtz-Zentrum für Infektionsforschung GmbH

  公开号：EP2230312A1

  公开（公告）日：2010-09-22

  The present invention relates to a probe compound that can comprise any substrate or metabolite of an enzymatic reaction in addition to an indicator component, such as, for example, a fluorescence dye, or the like. Moreover, the present invention relates to means for detecting enzymes in form of an array, which comprises any number of probe compounds of the invention which each comprise a different metabolite of interconnected metabolites representing the central pathways in all forms of life. Moreover, the present invention relates to a method for detecting enzymes involving the application of cell extracts or the like to the array of the invention which leads to reproducible enzymatic reactions with the substrates. These specific enzymatic reactions trigger the indicator (e.g. a fluorescence signal) and bind the enzymes to the respective cognate substrates. Moreover, the invention relates to means for isolating enzymes in form of nanoparticles coated with the probe compound of the invention. The immobilisation of the cognate substrates or metabolites on the surface of nanoparticles by means of the probe compounds allows capturing and isolating the respective enzyme, e.g. for subsequent sequencing.

  本发明涉及一种探针化合物，它可以包括酶反应的任何底物或代谢物，此外还包括指示成分，例如荧光染料或类似物。此外，本发明还涉及以阵列形式检测酶的方法，该阵列由任意数量的本发明探针化合物组成，每种探针化合物由代表所有生命形式中中心途径的相互关联的代谢物中的不同代谢物组成。此外，本发明还涉及一种检测酶的方法，该方法涉及将细胞提取物或类似物应用于本发明的阵列，从而导致与底物发生可重复的酶反应。这些特定的酶反应会触发指示剂（如荧光信号），并将酶与各自的同源底物结合。此外，本发明还涉及以涂覆有本发明探针化合物的纳米颗粒形式分离酶的方法。通过探针化合物将同源底物或代谢物固定在纳米颗粒表面，可以捕获和分离相应的酶，例如用于后续测序。
• Methods for conducting assays for enzyme activity on protein microarrays
  申请人：Zhou X. Fang

  公开号：US20050118665A1

  公开（公告）日：2005-06-02

  The present invention relates to methods of conducting assays for enzymatic activity on microarrays useful for the large-scale study of protein function, screening assays, and high-throughput analysis of enzymatic reactions. The invention relates to methods of using protein chips to assay the presence, amount, activity and/or function of enzymes present in a protein sample on a protein chip. In particular, the methods of the invention relate to conducting enzymatic assays using a microarray wherein a protein and a substance are immobilized on the surface of a solid support and wherein the protein and the substance are in proximity to each other sufficient for the occurrence of an enzymatic reaction between the substance and the protein. The invention also relates to microarrays that have an enzyme and a substrate immobilized on their surface wherein the enzyme and the substrate are in proximity to each other sufficient for the occurrence of an enzymatic reaction between the enzyme and the substrate.

  本发明涉及在微阵列上进行酶活性测定的方法，这种方法可用于蛋白质功能的大规模研究、筛选测定和酶反应的高通量分析。本发明涉及使用蛋白质芯片检测蛋白质芯片上蛋白质样品中酶的存在、数量、活性和/或功能的方法。特别是，本发明的方法涉及使用芯片进行酶测定，其中蛋白质和物质被固定在固体支持物表面，蛋白质和物质相互靠近，足以使物质和蛋白质之间发生酶反应。本发明还涉及在其表面固定有酶和底物的微阵列，其中酶和底物相互靠近，足以使酶和底物之间发生酶反应。
• BIOSENSORS, REAGENTS AND DIAGNOSTIC APPLICATIONS OF DIRECTED EVOLUTION
  申请人：Maxygen, Inc.

  公开号：EP1373889A2

  公开（公告）日：2004-01-02