N-[9-[(3R,4R,5R,6R)-4-[tert-butyl(dimethyl)silyl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-3-yl]-6-oxo-1H-purin-2-yl]-2-methylpropanamide | 205382-59-8

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 咪唑并嘧啶类
• 英文名称: N-[9-[(3R,4R,5R,6R)-4-[tert-butyl(dimethyl)silyl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-3-yl]-6-oxo-1H-purin-2-yl]-2-methylpropanamide
• CAS: 205382-59-8
• 化学式: C₂₁H₃₅N₅O₆Si
• 分子量: 481.624
• InChiKey: GFKQXQFBRJVFPA-RRCSTGOVSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  1.4
• 重原子数：
  33
• 可旋转键数：
  7
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.71
• 拓扑面积：
  147
• 氢给体数：
  4
• 氢受体数：
  8

反应信息

• 作为反应物：
  描述：

  N-[9-[(3R,4R,5R,6R)-4-[tert-butyl(dimethyl)silyl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-3-yl]-6-oxo-1H-purin-2-yl]-2-methylpropanamide 在 N,N-二异丙基乙胺 作用下， 以 吡啶 、 二氯甲烷 为溶剂， 反应 2.0h， 生成 Diisopropyl-phosphoramidous acid (2R,3R,4R,5R)-4-(tert-butyl-dimethyl-silanyloxy)-5-(2-isobutyrylamino-6-oxo-1,6-dihydro-purin-9-yl)-2-[(4-methoxy-phenyl)-diphenyl-methoxymethyl]-tetrahydro-pyran-3-yl ester 2-cyano-ethyl ester
  参考文献：
  名称：

  Oligonucleotides Composed of 2‘-Deoxy-1‘,5‘-anhydro-d-mannitol Nucleosides with a Purine Base Moiety

  摘要：

  2'-Deoxy-D-mannitol nucleosides with a purine base moiety have been conveniently synthesized starting from 1,5-anhydro-4,6-O-benzylidene-D-glucito The 3-OH function of 1,5-anhydro-4,6-O-benzylidene-D-glucitol was selectively protected with tert-butyldimethylsilyl group, and the 2'-OH function was subsequently converted to the corresponding O-triflate derivative for the introduction of the nucleobase moieties. These nucleoside derivatives were transformed to 1,5-anhydro-4-O-(P-(2 -cyanoethyl)-P-(N,N-diisopropylamino)phosphinyl)-2-deoxy-6-O-monomethoxytrityl-3-O-(tert-butyldimethylsilyl)-D-mannitol with either a 2-(N(6)-benzoyladenin-9-yl) or a 2-(N(2)-isobutyrylguanin-9-yl) substituent as the building blocks for oligonucleotide synthesis. The corresponding fully modified oligonucleotides afford considerably less stable duplexes with RNA as compared to the 3-deoxy hexitol nucleic acid analogues described previously, The reason for the lower stability was investigated using molecular modeling, MD simulations of single strand MNA(GCGTAGCG) and MNA(GCGTAGCG) complexed with RNA(CGCAUCGC) in aqueous solution were performed by use of AMBER 4.1 with the particle mesh Ewald (PME) method for the treatment of long-range electrostatic interactions, Frequent hydrogen bonds between the 3'-hydroxyl and the 6'-O of the phosphate backbone of the following base changed the conformation of the single strand as well as the MNA:RNA complex. The MNA:RNA backbone widens up and shows partial unwinding and disruption of base pair hydrogen bonds consistent with their low hybridization potential.

  DOI：

  10.1021/jo9718511
• 作为产物：
  描述：

  1,5-anhydro-4,6-O-benzylidene-2-deoxy-2-(guanin-9-yl)-D-mannitol 在 吡啶 、 咪唑 、 三氟乙酸 作用下， 以 二氯甲烷 、 N,N-二甲基甲酰胺 为溶剂， 生成 N-[9-[(3R,4R,5R,6R)-4-[tert-butyl(dimethyl)silyl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-3-yl]-6-oxo-1H-purin-2-yl]-2-methylpropanamide
  参考文献：
  名称：

  Synthesis of 1,5-Anhydro-D-Mannitol Nucleosides with a Purine Base Moeety

  摘要：

  D-Mannitol nucleosides with a purine base moiety have been conveniently synthesized strating from 1,5-anhydro-4,6-O-benzylidene-D-glucitol. The 3-OH function of 1,5-anhydro-4,6-O-benzylidene-D-glucitol was selectively protected with t-butyldimethylsilyl group and subsequently converted to the corresponding O-triflate derivative for the introduction of the nucleobase moietes. These nucleoside derivatives were transformed to 1,5-Anhydro-6-O-MMTr-2-(N(6)-benzoyladenin-9-yl)-2-deoxy-3-O-TBPMS-D-mannitol and 1,5-Anhydro-6-O-MMTr-2-(N(2)-isobutyrylguanin-9-yl)-2-deoxy-3-O-TBDMS-D-mannitol, useful as the building blocks for oligonucleotide synthesis. Also, the synthesis of the corresponding fully deprotected anhydrohexitol nucleosides were achieved for evaluation of antiviral activity test.

  DOI：

  10.1080/07328319808004713

文献信息

• Oligonucleotides Composed of 2‘-Deoxy-1‘,5‘-anhydro-<scp>d</scp>-mannitol Nucleosides with a Purine Base Moiety
  作者：Nafizal Hossain、Berthold Wroblowski、Arthur Van Aerschot、Jef Rozenski、Andre De Bruyn、Piet Herdewijn

  DOI：10.1021/jo9718511

  日期：1998.3.1

  2'-Deoxy-D-mannitol nucleosides with a purine base moiety have been conveniently synthesized starting from 1,5-anhydro-4,6-O-benzylidene-D-glucito The 3-OH function of 1,5-anhydro-4,6-O-benzylidene-D-glucitol was selectively protected with tert-butyldimethylsilyl group, and the 2'-OH function was subsequently converted to the corresponding O-triflate derivative for the introduction of the nucleobase moieties. These nucleoside derivatives were transformed to 1,5-anhydro-4-O-(P-(2 -cyanoethyl)-P-(N,N-diisopropylamino)phosphinyl)-2-deoxy-6-O-monomethoxytrityl-3-O-(tert-butyldimethylsilyl)-D-mannitol with either a 2-(N(6)-benzoyladenin-9-yl) or a 2-(N(2)-isobutyrylguanin-9-yl) substituent as the building blocks for oligonucleotide synthesis. The corresponding fully modified oligonucleotides afford considerably less stable duplexes with RNA as compared to the 3-deoxy hexitol nucleic acid analogues described previously, The reason for the lower stability was investigated using molecular modeling, MD simulations of single strand MNA(GCGTAGCG) and MNA(GCGTAGCG) complexed with RNA(CGCAUCGC) in aqueous solution were performed by use of AMBER 4.1 with the particle mesh Ewald (PME) method for the treatment of long-range electrostatic interactions, Frequent hydrogen bonds between the 3'-hydroxyl and the 6'-O of the phosphate backbone of the following base changed the conformation of the single strand as well as the MNA:RNA complex. The MNA:RNA backbone widens up and shows partial unwinding and disruption of base pair hydrogen bonds consistent with their low hybridization potential.
• Synthesis of 1,5-Anhydro-D-Mannitol Nucleosides with a Purine Base Moeety
  作者：Nafizal Hossain、Piet Herdewijn

  DOI：10.1080/07328319808004713

  日期：1998.9

  D-Mannitol nucleosides with a purine base moiety have been conveniently synthesized strating from 1,5-anhydro-4,6-O-benzylidene-D-glucitol. The 3-OH function of 1,5-anhydro-4,6-O-benzylidene-D-glucitol was selectively protected with t-butyldimethylsilyl group and subsequently converted to the corresponding O-triflate derivative for the introduction of the nucleobase moietes. These nucleoside derivatives were transformed to 1,5-Anhydro-6-O-MMTr-2-(N(6)-benzoyladenin-9-yl)-2-deoxy-3-O-TBPMS-D-mannitol and 1,5-Anhydro-6-O-MMTr-2-(N(2)-isobutyrylguanin-9-yl)-2-deoxy-3-O-TBDMS-D-mannitol, useful as the building blocks for oligonucleotide synthesis. Also, the synthesis of the corresponding fully deprotected anhydrohexitol nucleosides were achieved for evaluation of antiviral activity test.