The multilayer test devices of this invention comprise, in order, (a) an absorbent layer; (b) a waterproof barrier layer; and (c) a quickly absorbent reagent matrix layer having a determinate volume. The absorbent and barrier layers each include an aperture functionally aligned for application of a test sample through the absorbent and barrier layers and onto the surface of the reagent matrix layer. The reagent matrix layer contains one or more reagents which produce a detectable species in the presence of an analyte. The reagent matrix layer preferably comprises an asymmetrical filter, which localizes cellular components of the test sample at or near the surface of the filter, while facilitating saturation of the filter by non-cellular sample components. Preferred embodiments of the test devices include devices for testing glucose, alcohol and cholesterol in whole blood.
本发明的多层测试装置依次包括:(a) 吸收层;(b) 防
水阻隔层;(c) 具有确定体积的快速吸收试剂基质层。吸收层和阻隔层各包括一个在功能上对齐的孔,用于将测试样品穿过吸收层和阻隔层,涂抹到试剂基质层的表面上。试剂基质层含有一种或多种试剂,这些试剂在分析物存在的情况下会产生可检测的物质。试剂基质层最好包括一个不对称的过滤器,它能将测试样本的细胞成分定位在过滤器表面或其附近,同时促进非细胞样本成分对过滤器的饱和。检测设备的优选实施方案包括用于检测全血中
葡萄糖、
酒精和
胆固醇的设备。