Chloramphenicol-ditrimethylsilylaether | 21196-84-9

• 分子结构分类: 有机化合物 - 苯类化合物 - 苯和取代衍生物
• 英文名称: Chloramphenicol-ditrimethylsilylaether
• 英文别名: Bis-trimethylsilyl-chloramphenicol；Acetamide, 2,2-dichloro-N-[2-(4-nitrophenyl)-2-[(trimethylsilyl)oxy]-1-[[(trimethylsilyl)oxy]methyl]ethyl]-, [R-(R*,R*)]-；2,2-dichloro-N-[(1R,2R)-1-(4-nitrophenyl)-1,3-bis(trimethylsilyloxy)propan-2-yl]acetamide
• CAS: 21196-84-9
• 化学式: C₁₇H₂₈Cl₂N₂O₅Si₂
• 分子量: 467.497
• InChiKey: BRZXONFKPHDZOK-HUUCEWRRSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  4.63
• 重原子数：
  28
• 可旋转键数：
  9
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.59
• 拓扑面积：
  93.4
• 氢给体数：
  1
• 氢受体数：
  5

反应信息

• 作为产物：
  描述：

  N,O-双三甲硅基乙酰胺 、 氯霉素 以 乙腈 为溶剂， 反应 0.17h， 生成 Chloramphenicol-ditrimethylsilylaether
  参考文献：
  名称：

  Detection of Residual Chloramphenicol, Florfenicol, and Thiamphenicol in Yellowtail Fish Muscles by Capillary Gas Chromatography−Mass Spectrometry

  摘要：

  Chloramphenicol (CAP), florfenicol (FF), and thiamphenicol (TAP) were extracted from yellowtail muscles with ethyl acetate, and the extract was evaporated. The residues was dissolved with sodium chloride solution and partitioned with n-hexane to remove lipids, and then the drugs were extracted with ethyl acetate. After evaporation of ethyl acetate extract, the residue was dissolved with n-hexane followed by ethyl ether and applied to a Sep-Pak Florisil cartridge successively. The drugs were eluted from the cartridge with methanol-ethyl ether (3:7), and eluate was evaporated to dryness. After acetonitrile and BSA were added to the residues, the drugs were derivatized at 50 degrees C for 10 min. After the solvent was evaporated, the residue was dissolved with ethyl acetate and applied to gas chromatography-mass spectrometry. The drugs were separated by a capillary column coated with 5% phenyl methyl silicone, and SIM was performed at m/z 208 and 225 for CAP, at m/z 257 for FF, and at m/z 242, 257, and 330 for TAP. Recoveries of each drug from yellowtail muscle fortified at 0.1 ppm were more than 65%, and detection limits were 5 ppb.

  DOI：

  10.1021/jf950343u

文献信息

• Matrix Solid-Phase Dispersion Extraction and Gas Chromatographic Determination of Chloramphenicol in Muscle Tissue
  作者：Helena Kubala-Drincic、Davorin Bazulic、Jasenka Sapunar-Postruznik、Mirela Grubelic、Goran Stuhne

  DOI：10.1021/jf025939d

  日期：2003.2.1

  A method based on matrix solid-phase dispersion (MSPD) was developed for the gas chromatographic (GC) determination of chloramphenicol (CAP) residues in animal muscle tissue. Muscle tissue was blended with octadecylsilyl-derivatized silica (C(18)). A column made from the C(18)/muscle tissue matrix was washed with n-hexane and acetonitrile/water (5 + 95), after which CAP was eluted with acetonitrile/water

  建立了一种基于基质固相分散体（MSPD）的方法，用于气相色谱法（GC）测定动物肌肉组织中的氯霉素（CAP）残留量。将肌肉组织与十八烷基甲硅烷基衍生的二氧化硅（C（18））混合。用正己烷和乙腈/水（5 + 95）洗涤由C（18）/肌肉组织基质制成的柱子，然后用乙腈/水（50 + 50）洗脱CAP，并分配到乙酸乙酯中。蒸发最终的提取物，并用Sylon HTP制备CAP的三甲基甲硅烷基衍生物，并通过具有电子捕获检测器（ECD）和质谱仪的GC进行检测。为了定量，使用的内标是GC-ECD的CAP的同分异构体（m-CAP）。在三个浓度水平下强化肌肉组织样品。5、10 和15 microg / kg的水平，各自的平均回收率分别为93、96和98％，重复性分别为13、11和3％。ECD的检出限和定量限分别为1.6和4.0 microg / kg。通过MSPD技术从各种动物（牛，猪和家禽）的肌肉组织中提取C