fluorescein 3-O-butyl ether n-butyl ester | 131205-61-3

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 苯并吡喃
• 英文名称: fluorescein 3-O-butyl ether n-butyl ester
• 英文别名: Butyl 2-(6-butoxy-3-oxo-3H-xanthen-9-YL)benzoate；butyl 2-(3-butoxy-6-oxoxanthen-9-yl)benzoate
• CAS: 131205-61-3
• 化学式: C₂₈H₂₈O₅
• 分子量: 444.527
• InChiKey: XVAZWZYIMKSFCV-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  5.6
• 重原子数：
  33
• 可旋转键数：
  10
• 环数：
  4.0
• sp3杂化的碳原子比例：
  0.29
• 拓扑面积：
  61.8
• 氢给体数：
  0
• 氢受体数：
  5

反应信息

• 作为反应物：
  描述：

  fluorescein 3-O-butyl ether n-butyl ester 在 sodium hydroxide 、 三乙胺 作用下， 以 甲醇 、 二氯甲烷 、 水 为溶剂， 反应 2.0h， 生成 6'-O-(N-maleimido-DL-3-amino-2-methylpropionyl)-3'-O-butylfluorescein
  参考文献：
  名称：

  General strategy for the preparation of membrane permeable fluorogenic peptide ester conjugates for in vivo studies of ester prodrug stability

  摘要：

  To study ester prodrug stability properties in living cells we have conjugated fluorogenic esters to the cell membrane permeable peptide Arg(9). The desired conjugates are prepared by coupling N-maleoyl amino acid esters of monoalkylated fluoresceins or fluorescein to TyrArg(9)Cys. The photophysical properties of the monoalkylated fluorescein derivatives are described. (C) 2003 Elsevier Ltd. All rights reserved.

  DOI：

  10.1016/j.bmc.2003.11.016
• 作为产物：
  描述：

  正溴丁烷 、 fluorescein disodium salt 以 N,N-二甲基甲酰胺 为溶剂， 反应 24.0h， 生成 fluorescein 3-O-butyl ether n-butyl ester
  参考文献：
  名称：

  General strategy for the preparation of membrane permeable fluorogenic peptide ester conjugates for in vivo studies of ester prodrug stability

  摘要：

  To study ester prodrug stability properties in living cells we have conjugated fluorogenic esters to the cell membrane permeable peptide Arg(9). The desired conjugates are prepared by coupling N-maleoyl amino acid esters of monoalkylated fluoresceins or fluorescein to TyrArg(9)Cys. The photophysical properties of the monoalkylated fluorescein derivatives are described. (C) 2003 Elsevier Ltd. All rights reserved.

  DOI：

  10.1016/j.bmc.2003.11.016

文献信息

• Optically-Detectable Enzyme Substrates and Their Method of Use
  申请人：Life Technologies Corporation

  公开号：US20160139135A1

  公开（公告）日：2016-05-19

  The present invention relates to compounds that are substrates for an enzyme, and upon reaction with the enzyme provide a detectable response, such as an optically detectable response. In particular, the compounds have utility in detecting the presence of a β-lactamase in a sample. In addition to the compounds, methods are disclosed for analyzing a sample for the presence of a β-lactmase, for example, as an indicator of expression of a nucleic acid sequence including a sequence coding for a β-lactmase. Kits are disclosed that include the disclosed compounds and additional components, for example, cells, antibodies, a β-lactmase or instructions for using the components in an assay.

  本发明涉及一种酶底物化合物，与酶反应后产生可检测的响应，例如光学可检测的响应。具体而言，这些化合物在检测样品中β-内酰胺酶的存在方面具有实用性。除了这些化合物外，还公开了用于分析样品中β-内酰胺酶存在的方法，例如，作为表达包括编码β-内酰胺酶序列的核酸序列的指示器。还公开了包括这些化合物和额外组分的试剂盒，例如细胞、抗体、β-内酰胺酶或使用这些组分进行检测的说明书。
• Aggregation-induced emission and solid fluorescence of fluorescein derivatives
  作者：Shumin Feng、Shengyi Gong、Guoqiang Feng

  DOI：10.1039/c9cc09784h

  日期：——

  The AIE and strong solid fluorescence of fluorescein derivatives can be realized in a simple way.

  荧光素衍生物的AIE和强固体荧光可以通过简单的方法实现。
• Fluorescein–fullerene dyads: a new kind of fullerene dyad—synthesis and their photophysical properties
  作者：Bingwen Jing、Deqing Zhang、Daoben Zhu

  DOI：10.1016/s0040-4039(00)01523-9

  日期：2000.10

  Two novel fluorescein–C60 dyads have been synthesized. Fluorescence quenching in the dyads indicates a photoinduced intramolecular electron transfer from the fluorescein to the C60 moiety.

  已经合成了两个新颖的荧光素-C 60二联体。二单元组中的荧光猝灭表明光诱导的分子内电子从荧光素转移到C 60部分。
• OPTICALLY-DETECTABLE ENZYME SUBSTRATES AND THEIR METHOD OF USE
  申请人：CORRY Schuyler Boon

  公开号：US20090047692A1

  公开（公告）日：2009-02-19

  The present invention relates to compounds that are substrates for an enzyme, and upon reaction with the enzyme provide a detectable response, such as an optically detectable response. In particular, the compounds have utility in detecting the presence of a β-lactamase in a sample. In addition to the compounds, methods are disclosed for analyzing a sample for the presence of a β-lactmase, for example, as an indicator of expression of a nucleic acid sequence including a sequence coding for a β-lactmase. Kits are disclosed that include the disclosed compounds and additional components, for example, cells, antibodies, a β-lactmase or instructions for using the components in an assay.

  本发明涉及一种酶底物化合物，经过与该酶的反应后提供可检测的响应，例如光学可检测响应。特别是，这些化合物在检测样品中β-内酰胺酶的存在方面具有实用性。除了这些化合物，还揭示了一种分析样品中β-内酰胺酶存在的方法，例如，作为包括编码β-内酰胺酶的核酸序列的表达的指示器。还揭示了包括上述化合物和其他组分的试剂盒，例如细胞、抗体、β-内酰胺酶或使用这些组分进行检测的说明。
• US8318450B2
  申请人：——

  公开号：US8318450B2

  公开（公告）日：2012-11-27