2,5-dioxopyrrolidin-1-yl (4-((2-(diethylamino)ethyl)carbamoyl)phenyl)carbamate

• 分子结构分类: 有机化合物 - 苯类化合物 - 苯和取代衍生物
• 英文名称: 2,5-dioxopyrrolidin-1-yl (4-((2-(diethylamino)ethyl)carbamoyl)phenyl)carbamate
• 英文别名: (2,5-dioxopyrrolidin-1-yl) N-[4-[2-(diethylamino)ethylcarbamoyl]phenyl]carbamate
• 化学式: C₁₈H₂₄N₄O₅
• 分子量: 376.412
• InChiKey: LXNNLXYTXAKUOO-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  0.7
• 重原子数：
  27
• 可旋转键数：
  9
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.44
• 拓扑面积：
  108
• 氢给体数：
  2
• 氢受体数：
  6

反应信息

• 作为产物：
  描述：

  N,N'-二琥珀酰亚胺基碳酸酯 、 普鲁卡因胺 以 乙腈 为溶剂， 反应 5.0h， 生成 2,5-dioxopyrrolidin-1-yl (4-((2-(diethylamino)ethyl)carbamoyl)phenyl)carbamate
  参考文献：
  名称：

  強められたＭＳ信号を有するグリカンおよび他の生体分子の迅速蛍光タグ付け

  摘要：

  提供具有增强的质谱信号的糖蛋白和其他生物分子的快速荧光标记。具有对与生物分子如N-糖蛋白等进行标记反应的活化官能性的质谱活性、包含荧光分子的试剂以及其使用方法被揭示和描述。【选定图】无

  公开号：

  JP2016041686A

文献信息

• RAPID FLUORESCENCE TAGGING OF GLYCANS AND OTHER BIOMOLECULES WITH ENHANCED MASS SPECTROMETRY SIGNALS
  申请人：Waters Technologies Corporation

  公开号：EP2990401A1

  公开（公告）日：2016-03-02

  Reagents comprising MS active, fluorescent molecules with an activated functionality for reaction with amines useful in tagging biomolecules such as N-glycans and uses thereof are taught and described.

  本文教导并描述了由 MS 活性荧光分子组成的试剂及其用途，这些荧光分子具有与胺反应的活化功能，可用于标记 N-聚糖等生物大分子。
• CALIBRANT FOR LIQUID CHROMATOGRAPHY CALIBRATION OF LABELED N-GLYCANS
  申请人：Waters Technologies Corporation

  公开号：EP3744708A1

  公开（公告）日：2020-12-02

  Methods are provided for making rapid labeled dextran ladders and other calibrants useful in liquid chromatography. The methodologies include a two-step process comprising a reductive amination step of providing a reducing glycan and reacting it with a compound having a primary amine to produce an intermediate compound. The intermediate compound is then rapidly tagged with a rapid tagging reagent to produce the rapid labeled dextran ladder.

  本发明提供了用于液相色谱法中的快速标记葡聚糖阶梯和其他校准物的制造方法。这些方法包括一个还原胺化步骤和两个步骤，还原胺化步骤是提供还原性聚糖并使其与具有伯胺的化合物反应生成中间化合物。然后用快速标记试剂对中间化合物进行快速标记，生成快速标记葡聚糖梯形图。
• Rapid fluorescence tagging of glycans and other biomolecules with enhanced MS signals
  申请人：Waters Technologies Corporation

  公开号：US10416166B2

  公开（公告）日：2019-09-17

  Reagents comprising MS active, fluorescent molecules with an activate functionality for reaction with amines useful in rapid tagging of biomolecules such as N-glycans, proteins, peptides, and amino acids, and uses thereof are taught and described. These MS active, fluorescent molecules may have three functional components such as a tertiary amino group or other MS active atom, a highly fluorescent moiety, and a functional group that rapidly reacts with amines.

  本文教导并描述了由 MS 活性荧光分子组成的试剂，这些分子具有与胺反应的活化功能，可用于快速标记 N-聚糖、蛋白质、肽和氨基酸等生物大分子及其用途。这些具有 MS 活性的荧光分子可能具有三种功能成分，如三级氨基或其他 MS 活性原子、高荧光分子和可与胺快速反应的功能基团。
• Methods for the rapid preparation of labeled glycosylamines and for the analysis of glycosylated biomolecules producing the same
  申请人：Waters Technologies Corporation

  公开号：US11371996B2

  公开（公告）日：2022-06-28

  Methods of analyzing glycosylated biomolecules include the steps of producing a deglycosylation mixture of biomolecules deglycosylated by natural or synthetic enzymatic or chemical techniques; providing a reagent solution having a labeling reagent in a polar aprotic, non-nucleophilic organic solvent; and mixing the deglycosylation mixture with the reagent solution in an excess of labeling reagent to produce derivatized glycosylamines. The method steps can be carried out purposefully without depletion of protein matter. A quenching solution can be added to the reaction mixture so that the pH of the reaction mixture is shifted to above 10. The yield of derivatized glycosylamines can be in an amount of about 80 to about 100 mole percent of the reaction mixture with minimal overlabeling, less than 0.2 mole percent. The derivizated glycosylamines can be separated from the reaction mixture and detected by chromatographic detection, fluorescence detection, mass spectrometry (“MS”), or Ultra Violet (“UV”) detection and/or a combination thereof.

  分析糖基化生物大分子的方法包括以下步骤：制备由天然或合成酶或化学技术脱糖的生物大分子组成的脱糖混合物；在极性非亲核有机溶剂中提供具有标记试剂的试剂溶液；以及将脱糖混合物与标记试剂过量的试剂溶液混合，生成衍生化的糖基化胺。这些方法步骤可以在不损耗蛋白质物质的情况下有目的地进行。可向反应混合物中加入淬灭溶液，使反应混合物的 pH 值升至 10 以上。衍生化糖基胺的产率可达到反应混合物的约 80 至约 100 摩尔%，且重叠标记最小，小于 0.2 摩尔%。衍生化的糖基胺可以从反应混合物中分离出来，并通过色谱检测、荧光检测、质谱（"MS"）或紫外线（"UV"）检测和/或它们的组合进行检测。
• METHODS FOR THE RAPID PREPARATION OF LABELED GLYCOSYLAMINES AND FOR THE ANALYSIS OF GLYCOSYLATED BIOMOLECULES PRODUCING THE SAME
  申请人：Waters Technologies Corporation

  公开号：EP3213056B1

  公开（公告）日：2020-08-12