4-[Bis(3-carboxybutyl)phosphanyl]-2-methylbutanoic acid

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 英文名称: 4-[Bis(3-carboxybutyl)phosphanyl]-2-methylbutanoic acid
• 英文别名: 4-[bis(3-carboxybutyl)phosphanyl]-2-methylbutanoic acid
• 化学式: C₁₅H₂₇O₆P
• 分子量: 334.34
• InChiKey: IDHHIGCCDDSMHH-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  1.2
• 重原子数：
  22
• 可旋转键数：
  12
• 环数：
  0.0
• sp3杂化的碳原子比例：
  0.8
• 拓扑面积：
  112
• 氢给体数：
  3
• 氢受体数：
  6

文献信息

• METHOD FOR PRODUCING POLYPEPTIDE FRAGMENT WITH HIGH EFFICIENCY, WHICH IS SUITABLE FOR NCL METHOD
  申请人：Glytech, Inc.

  公开号：EP2762485A1

  公开（公告）日：2014-08-06

  [Problem] The object of the present invention is to provide a method for efficiently manufacturing a polypeptide fragment suitable for the NCL method. [Solution] The present invention provides a manufacturing method comprising a step of reacting a polypeptide consisting of a first polypeptide fragment having cysteine at the N-terminal and a second polypeptide fragment linked via an intervening sequence -Cys-W-(His)n-Z-Met- with CNBr to obtain a first polypeptide fragment having cysteine at the N-terminal and a third polypeptide fragment, and a step of sequentially reacting the third polypeptide fragment with a compound represented by the following formula (I) and a compound represented by the following formula (II) to obtain a second polypeptide fragment having the C-terminal modified.

  [问题]本发明的目的是提供一种高效制造适用于 NCL 法的多肽片段的方法。[解决方案] 本发明提供了一种制造方法，包括以下步骤：将由 N 端具有半胱氨酸的第一 多肽片段和通过中间序列-Cys-W-(His)n-Z-Met-连接的第二多肽片段组成的多肽与 CNBr 反应，得到 N 端具有半胱氨酸的第一多肽片段和第三多肽片段、以及将第三多肽片段依次与下式(I)代表的化合物和下式(II)代表的化合物反应，得到 C-末端被修饰的第二多肽片段的步骤。