[EN] ENCODED NANOPORE SENSOR FOR MULTIPLEX NUCLEIC ACIDS DETECTION<br/>[FR] CAPTEUR À NANOPORES CODÉS POUR LA DÉTECTION MULTIPLEXÉE D'ACIDES NUCLÉIQUES
申请人:UNIV MISSOURI
公开号:WO2014144217A1
公开(公告)日:2014-09-18
The present invention provides a new and improved multiplexed oligonucleotide detection method based on the nanopore technology with one or more probes containing a sequence with complementarity to the target oligonucleotide, a terminal extension at the probe's 3' terminus, 5' terminus, or both termini and a label attached to the terminus. The improved probes and probe sets enable sensitive, selective, and direct multiplex detection, differentiation and quantification of distinct target oligonucleotides such as miRNAs. The inventive detection method may also be employed as a non-invasive and cost-effective diagnostic method based on miRNA levels in the patient's tissue sample.