6-amino-N-[3-(5-nitro-1H-pyrrol-3-yl)prop-2-ynyl]hexanamide

• 分子结构分类: 有机化合物 - 有机1,3-偶极化合物 - 烯丙基型1,3-偶极有机化合物
• 英文名称: 6-amino-N-[3-(5-nitro-1H-pyrrol-3-yl)prop-2-ynyl]hexanamide
• 化学式: C13H18N4O3
• 分子量: 278.31
• InChiKey: GAXDJMMNEIBEQH-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  0.4
• 重原子数：
  20
• 可旋转键数：
  7
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.46
• 拓扑面积：
  117
• 氢给体数：
  3
• 氢受体数：
  4

文献信息

• Methods and systems for high resolution fluorescence microscopy of polymeric dye-labeled samples using polarized light
  申请人：Becton, Dickinson and Company

  公开号：US10408759B2

  公开（公告）日：2019-09-10

  Methods for producing a high resolution image of a sample are provided. In some embodiments, the method includes: detecting first and second sets of spatially-dependent emission signals from a sample labeled with a fluorescent polymeric dye; and producing a high resolution fluorescence image of the sample from the detected first and second sets of spatially-dependent emission signals. In some embodiments, the sample is a cell. Also provided are systems for imaging a sample that include a high resolution light microscope including a light source configured to irradiate a field of view with an excitation light; a photodetector configured to detect an emission signal: and a polarization modulator disposed in the light pathway between the light source and the photodetector; and a sample labelled with a polymeric dye and disposed in the field of view.

  本文提供了制作样品高分辨率图像的方法。在一些实施例中，该方法包括：检测用荧光聚合物染料标记的样品的第一和第二组空间依赖性发射信号；以及根据检测到的第一和第二组空间依赖性发射信号生成样品的高分辨率荧光图像。在某些实施例中，样品是细胞。还提供了用于样品成像的系统，该系统包括高分辨率光学显微镜，其中包括：光源，用于用激发光照射视场；光电探测器，用于检测发射信号；偏振调节器，设置在光源和光电探测器之间的光通路中；以及用聚合染料标记并设置在视场中的样品。
• Polynucleotide sequencer tuned to artificial polynucleotides
  申请人：Microsoft Technology Licensing, LLC

  公开号：US10930370B2

  公开（公告）日：2021-02-23

  Artificial polynucleotides may have different characteristics than natural polynucleotides so conventional base-calling algorithms may make incorrect base calls. However, because artificial polynucleotides are typically designed to have certain characteristics, the known characteristics of the artificial polynucleotide can be used to modify the base-calling algorithm. This disclosure describes polynucleotide sequencers adapted to sequence artificial polynucleotides by modifying a base-calling algorithm of the polynucleotide sequencer according to known characteristics of the artificial polynucleotides. The base-calling algorithm analyzes raw data generated by a polynucleotide sequencer and identifies which nucleotide base occupies a given position on a polynucleotide strand.

  人工多核苷酸的特性可能与天然多核苷酸不同，因此传统的碱基调用算法可能会调用出错误的碱基。不过，由于人工多核苷酸通常被设计为具有某些特性，因此可以利用人工多核苷酸的已知特性来修改碱基调用算法。本公开描述了根据人造多核苷酸的已知特性修改多核苷酸测序仪的碱基调用算法，使之适于对人造多核苷酸进行测序的多核苷酸测序仪。碱基召唤算法分析多核苷酸测序仪生成的原始数据，并确定哪个核苷酸碱基占据多核苷酸链上的给定位置。
• Method for detecting protein-specific glycosylation
  申请人：The Regents of the University of California

  公开号：US11041850B2

  公开（公告）日：2021-06-22

  Methods are provided for detecting a glycosylated target protein in a sample. Aspects of the methods include: (a) contacting a sample comprising a probe-labeled glycosylated target protein with: (i) a first conjugate comprising a first nucleic acid tag linked to a first capture agent that specifically binds the target protein; (ii) a second conjugate comprising a second nucleic acid tag linked to a second capture agent that specifically binds the probe; and (iii) a bridging nucleic acid that hybridizes to the first and second nucleic acid tags; under conditions sufficient to specifically bind the first and second capture agents to the probe-labeled target protein and to hybridize the bridging nucleic acid to the first and second nucleic acid tags to produce a nucleic acid complex; and (b) detecting the nucleic acid complex. Also provided are compositions and kits useful in practicing various embodiments of the subject methods.

  提供了检测样品中糖基化目标蛋白质的方法。方法的各个方面包括(a) 将包含探针标记的糖基化目标蛋白的样品与以下物质接触：(i) 第一共轭物，包括与第一捕获剂连接的第一核酸标签，第一捕获剂特异性结合目标蛋白；(ii) 第二共轭物，包括与第二捕获剂连接的第二核酸标签，第二捕获剂特异性结合探针；以及 (iii) 桥接核酸，桥接核酸与第一和第二核酸标签杂交；在足以使第一和第二捕获剂与探针标记的靶蛋白特异性结合并使桥接核酸与第一和第二核酸标签杂交以产生核酸复合物的条件下；以及 (b) 检测核酸复合物。此外，还提供了用于实施本主题方法各种实施方案的组合物和试剂盒。
• Methods of quantifying target nucleic acids and identifying sequence variants
  申请人：OMNIOME, INC.

  公开号：US11326206B2

  公开（公告）日：2022-05-10

  The present disclosure provides compositions, methods and systems for quantifying target sequences and identifying target sequence variants.

  本公开提供了用于量化目标序列和识别目标序列变体的组合物、方法和系统。
• Gapmers and methods of using the same for the treatment of muscular dystrophy
  申请人：Children's National Medical Center

  公开号：US11518995B2

  公开（公告）日：2022-12-06

  The disclosure relates to compositions comprising a nucleotide sequence having two domains: a locked nucleic acid (LNA) domain and a DNA gap domain, wherein nucleotide sequence binds to an endogenous DUX4 mRNA sequence disrupts DUX4 expression.

  本公开涉及一种包含核苷酸序列的组合物，该核苷酸序列具有两个结构域：锁定核酸（LNA）结构域和 DNA 间隙结构域，其中核苷酸序列可与内源性 DUX4 mRNA 序列结合，从而破坏 DUX4 的表达。