申请人:Streck Laboratories, Inc.
公开号:EP1422509A1
公开(公告)日:2004-05-26
A reagent system for substantially lysing red blood cells in a whole blood sample prior to leukocyte analysis, the reagent system includes: a first reagent for substantially lysing the red blood cells in the whole blood sample, and a second reagent for quenching the activity of the first reagent, wherein the second reagent includes a base and has a pH value of about 8 to 12. A final acidic media, ranging from about pH 4 to about 6, is used to stabilize the white blood cells and continuously remove red blood cell fragments. The first reagent is formulated to include: a saponin compound; an acid selected from the group consisting of halogenated carboxylic acids, phosphoric acid or combinations thereof and optionally a surfactant. In addition a method of lysing the red blood cells present in a sample of whole blood, includes the steps of: combining a predetermined portion of the sample of whole blood with a predetermined portion of a first reagent for substantially lysing the red blood cells and stabilizing white blood cells in the whole blood sample, wherein the first reagent includes: a saponin compound; and an acid; and quenching the lysing action of said first reagent by the addition of a predetermined portion of a second reagent, wherein the second reagent includes a base and has a pH value of about 8 to about 12 to give a solution containing substantially lysed red blood cells, leukocytes and a pH value of about 3 to about 6.
一种试剂系统,用于在白细胞分析前基本裂解全血样本中的红细胞,该试剂系统包括:第一试剂,用于基本裂解全血样本中的红细胞;第二试剂,用于淬灭第一试剂的活性,其中第二试剂包括碱,pH 值约为 8 至 12。最后的酸性介质(pH 值约为 4 至 6)用于稳定白细胞并持续清除红细胞碎片。第一种试剂的配方包括:一种皂素化合物;一种选自卤代羧酸、磷酸或其组合的酸,以及可选的一种表面活性剂。此外,一种裂解存在于全血样品中的红细胞的方法包括以下步骤:将全血样品的预定部分与第一试剂的预定部分结合,以基本裂解全血样品中的红细胞并稳定白细胞,其中第一试剂包括:皂苷化合物;和一种表面活性剂:一种皂苷化合物;和一种酸;以及通过加入预定份量的第二试剂来淬灭所述第一试剂的裂解作用,其中第二试剂包括一种碱,pH 值约为 8 至 12,从而得到一种溶液,该溶液含有基本裂解的红细胞和白细胞,pH 值约为 3 至 6。