[EN] USE OF OSMOLYTES TO HEAT STABILISE LUCIFERASE AND/OR APYRASE<br/>[FR] UTILISATION D'OSMOLYTES AFIN DE THERMOSTABILISER DE LA LUCIFERASE ET/OU APYRASE
申请人:BIOTAGE AB
公开号:WO2004035818A1
公开(公告)日:2004-04-29
The present invention refers to the use of at least one osmolyte to heat stabilise at least one enzyme, such as luciferase and/or apyrase, that is used in an in-vitro bio-luminometric assay, such as a sequencing-by-synthesis reaction, especially Pyrose-quencingTM, during the enzymatic reaction. Moreover, the invention refers to a composition and a kit for use in the bioluminometric assay. Hereby, the temperature sensitive enzyme is heat stabilised, whereby the bioluminometric assay may be per-formed at a temperature that is higher and therefore more suitable for the other en-zymes of the assay.