6,8-Difluoro-4-methyl-2-oxo-2H-1-benzopyran-7-yl beta-D-galactopyranoside | 215868-26-1

• 分子结构分类: 有机化合物 - 苯丙烷和聚酮 - 香豆素及其衍生物
• 英文名称: 6,8-Difluoro-4-methyl-2-oxo-2H-1-benzopyran-7-yl beta-D-galactopyranoside
• 英文别名: 6,8-difluoro-4-methyl-7-[(2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxychromen-2-one
• CAS: 215868-26-1
• 化学式: C₁₆H₁₆F₂O₈
• 分子量: 374.29
• InChiKey: JZLOUZMEHMDFKY-DSERSLQPSA-N

物化性质

• 沸点：
  632.1±55.0 °C(Predicted)
• 密度：
  1.623±0.06 g/cm3(Predicted)
• 溶解度：
  可溶于DMSO

计算性质

• 辛醇/水分配系数(LogP)：
  -0.6
• 重原子数：
  26
• 可旋转键数：
  3
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.44
• 拓扑面积：
  126
• 氢给体数：
  4
• 氢受体数：
  10

文献信息

• Saccharide Fluorescent Substrates, Preparation Method and Uses Thereof
  申请人：Texier-Nogues Isabelle

  公开号：US20080299044A1

  公开（公告）日：2008-12-04

  The invention concerns saccharide type fluorescent enzymatic substrates comprising on the same saccharide unit a fluorophor F1 and an inhibitor of the fluorescence of F1, and their use for preparing a diagnostic reagent for in vivo functional imaging, as well as a diagnostic reagent containing at least such an enzymatic substrate.

  这项发明涉及糖类荧光酶底物，其在同一糖类单元上包含荧光色团F1和F1荧光抑制剂，并且它们用于制备用于体内功能成像的诊断试剂，以及含有至少这种酶底物的诊断试剂。
• Substrats fluorescents saccharidiques, leur procédé de préparation et leurs utilisations
  申请人：Commissariat à l'Energie Atomique

  公开号：EP2033663A1

  公开（公告）日：2009-03-11

  La présente invention est relative à des substrats enzymatiques fluorescents de nature saccharidique comportant un bras espaceur auto-sécable fonctionnalisé par un fluorophore F et par au moins un inhibiteur de la fluorescence de F, à leur utilisation à titre de réactif fluorescent pour la détection d'une activité enzymatique in vitro ou pour la préparation d'un réactif de diagnostic destiné à l'imagerie fonctionnelle in vivo.

  本发明涉及具有糖性质的荧光酶底物，该底物包含由荧光团 F 和至少一种 F 荧光抑制剂官能化的自分离间隔臂，本发明还涉及将其用作荧光试剂，以检测体外酶活性或制备用于体内功能成像的诊断试剂。
• Biological indicator
  申请人：American Sterilizer Company

  公开号：US10011843B2

  公开（公告）日：2018-07-03

  This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.

  本发明涉及一种生物指示剂，其成分包括：由形成孢子的细菌组成的宿主生物；产生指示剂酶的报告基因；调控基因；以及将报告基因和调控基因插入宿主生物的载体；宿主生物的基因组中含有转座遗传因子，用于在调控基因中插入插入序列；插入序列包括转座酶、一对末端倒置重复序列和至少一个用于表达转座酶的开放阅读框。载体可被宿主生物吸收。插入序列可插入调控基因中。宿主生物可进行孢子繁殖以形成生物指示剂。本发明公开了一种使用生物指示剂的工艺和装置。
• Structurally biased random peptide libraries based on different scaffolds
  申请人：Rigel Pharmaceuticals, Inc.

  公开号：US20030143562A1

  公开（公告）日：2003-07-31

  The invention relates to the use of scaffold proteins, particularly green fluorescent protein (GFP), in fusion constructs with random and defined peptides and peptide libraries, to increase the cellular expression levels, decrease the cellular catabolism, increase the conformational stability relative to linear peptides, and to increase the steady state concentrations of the library peptides and peptide library members expressed in cells for the purpose of detecting the presence of the peptides and screening peptide libraries. N-terminal, C-terminal, dual N- and C-terminal and one or more internal fusions are all contemplated. Novel fusions utilizing self-binding peptides to create a conformationally stabilized fusion domain are also contemplated.

  本发明涉及将支架蛋白，特别是绿色荧光蛋白（GFP），用于随机肽和定义肽以及肽文库的融合构建物中，以提高细胞表达水平，减少细胞分解，增加相对于线性肽的构象稳定性，并提高在细胞中表达的文库肽和肽文库成员的稳态浓度，从而达到检测肽的存在和筛选肽文库的目的。N 端、C 端、双 N 端和 C 端以及一个或多个内部融合都是可以考虑的。此外，还考虑利用自结合肽的新型融合来创建构象稳定的融合结构域。
• Methods, compositions, and kits for analysis of enzyme activity in cells
  申请人：Graham J. Ronald

  公开号：US20050244907A1

  公开（公告）日：2005-11-03

  In one aspect, the present disclosure relates to methods for detecting an activity of an enzyme in a cell. In some embodiments, the methods include contacting a cell with a liposome containing at least one substrate thereby facilitating introduction of the substrate into the cell. The substrate is capable of producing a detectable light signal when acted on by the enzyme, and the signal is detected. The methods can be used in screening agents that can inhibit or activate an enzyme activity. The methods can also be used in various downstream assays such the detection of interactions between intracellular proteins, screening for variants of an enzyme, and detection of various diseases. Compositions and kits for carrying out the various methods are also provided.

  在一个方面，本公开涉及检测细胞中酶活性的方法。在某些实施方案中，这些方法包括使细胞与含有至少一种底物的脂质体接触，从而促进底物进入细胞。底物在酶的作用下能够产生可检测的光信号，并对信号进行检测。这些方法可用于筛选可抑制或激活酶活性的制剂。这些方法还可用于各种下游检测，如检测细胞内蛋白质之间的相互作用、筛选酶的变体以及检测各种疾病。还提供了用于实施各种方法的组合物和试剂盒。