(2R)-6-amino-2-[bis(carboxymethyl)amino]hexanoic acid

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 英文名称: (2R)-6-amino-2-[bis(carboxymethyl)amino]hexanoic acid
• 化学式: C₁₀H₁₈N₂O₆
• 分子量: 262.26
• InChiKey: SYFQYGMJENQVQT-SSDOTTSWSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -5.3
• 重原子数：
  18
• 可旋转键数：
  10
• 环数：
  0.0
• sp3杂化的碳原子比例：
  0.7
• 拓扑面积：
  141
• 氢给体数：
  4
• 氢受体数：
  8

文献信息

• METHODS FOR DETECTING HIS-TAGGED PROTEINS USING NTA PROBES AND POLYACRYLAMIDE GEL ELECTROPHORESIS
  申请人：King Abdullah University of Science and Technology

  公开号：US20210373012A1

  公开（公告）日：2021-12-02

  Methods for detecting His-tagged proteins using metal ion-chelating nitrilotriacetate (NTA) probes and polyacrylamide gel electrophoresis (PAGE) are disclosed. In one embodiment, the method includes using a metal ion-loaded NTA probe coupled to a UV-excitable fluorophore with visible emission and the presence of His-tagged proteins in the sample is determined by exposing the gel following PAGE, to a UV-light source with naked human eye or bench camera visualization. The metal ion-loaded NTA-containing chelator head can be coupled to a fluorophore that is not UV-excitable (i.e., with the majority of emission and excitation in the visible region of the electromagnetic spectrum. The method includes separating proteins in a sample using PAGE, contacting the gel following electrophoresis with a composition containing a metal ion-loaded NTA probe coupled to the fluorophore, to allow binding of the probe to the His-tagged proteins, and detecting the presence of the probe and therefore of the His-tagged proteins.