7-glycidyl coumarin

• 分子结构分类: 有机化合物 - 苯丙烷和聚酮 - 香豆素及其衍生物
• 英文名称: 7-glycidyl coumarin
• 英文别名: 7-Glycidylcoumarin；7-(oxiran-2-ylmethyl)chromen-2-one
• 化学式: C₁₂H₁₀O₃
• 分子量: 202.21
• InChiKey: OXMRVMHJGYRKAH-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  1.9
• 重原子数：
  15
• 可旋转键数：
  2
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.25
• 拓扑面积：
  38.8
• 氢给体数：
  0
• 氢受体数：
  3

反应信息

• 作为产物：
  描述：

  7-羟基香豆素 、 环氧溴丙烷 在 sodium hydroxide 、 potassium carbonate 作用下， 以 正己烷 、 氯仿 、 乙酸乙酯 、 丙酮 为溶剂， 生成 7-glycidyl coumarin
  参考文献：
  名称：

  Non-nucleosidic coumarin derivatives as polynucleotide-crosslinking

  摘要：

  本发明揭示了一种包含香豆素基团连接到非核苷骨架基团的新型香豆素衍生物。所得分子通常用作光活化交联基团，当它们替换掉探针中存在的一种或多种互补核苷酸碱基，被纳入到多核苷酸中，用于涉及核酸杂交反应的操作。

  公开号：

  US06005093A1

文献信息

• Non-nucleosidic coumarin derivatives as polynucleotide-crosslinking agents
  申请人：Naxcor, Inc.

  公开号：US06800768B1

  公开（公告）日：2004-10-05

  Novel coumarin derivatives comprising a coumarin moiety linked to a non-nucleosidic backbone moiety are disclosed. The resulting molecules are typically used as photoactivate crosslinking groups when incorporated into polynucleotides as replacements for one or more of the complementary nucleoside bases present in probes used in procedures involving nucleic acid hybridization reactions.

  本发明公开了一种新型香豆素衍生物，其包含一个香豆素基团与一个非核苷骨架基团相连。所得分子通常用作光致交联基团，当其替换掉核酸杂交反应中探针中存在的一个或多个互补核苷酸碱基时，被引入到多核苷酸中。
• Nucleic acid sequence detection employing amplification probes
  申请人：Naxcor

  公开号：US20030039961A1

  公开（公告）日：2003-02-27

  Methods and compositions are provided for detecting nucleic acid sequences. In particular, pairs of probes are employed, where the pair defines a substantially contiguous sequence on a target nucleic acid. Each of the pairs has a side chain which forms a stem of the two side chains which non-covalently binds and is capable of forming a cross-link upon activation, when the probes and sample nucleic acid are base paired. Cross-linking of the stems when unbound to complementary DNA is inhibited. Each of the nucleic acids is initially present as single stranded nucleic acid to allow for base pairing, so that the probes bind to homologous target nucleic acid. The assay mixture is activated to provide cross-linking, the double stranded nucleic acid melted, and the process of base pairing, activation and melting repeated, a sufficient number of cycles, to provide a detectable amount of cross-linked probes. To inhibit background cross-linking, the side chains may provide for duplex formation, where a portion of the side chain binds to a different portion of the side chain or the portion of the probe homologous to the target. Also provided are kits comprising reagents, as well as automatic devices, for carrying out the subject method.

  提供了检测核酸序列的方法和组合物。特别是采用了成对探针，其中成对探针定义了目标核酸上基本连续的序列。每对探针都有一条侧链，当探针和样本核酸碱基配对时，两条侧链形成一个茎，茎与探针非共价结合，并能在活化时形成交联。未与互补 DNA 结合时，茎的交联会受到抑制。每种核酸最初都是单链核酸，以便进行碱基配对，从而使探针与同源靶核酸结合。检测混合物被激活以产生交联，双链核酸被融化，碱基配对、激活和融化的过程重复足够多的循环，以提供可检测到的交联探针量。为抑制背景交联，侧链可提供双链形成，其中侧链的一部分与侧链的不同部分或探针与目标同源的部分结合。此外，还提供了包含试剂的试剂盒以及自动装置，用于实施主题方法。
• Nucleic acid sequence detection employing probes comprising non-nucleosidic coumarin derivatives as polynucleotide-crosslinking agents
  申请人：——

  公开号：US20030134274A1

  公开（公告）日：2003-07-17

  Methods and compositions are provided for detecting nucleic acid sequences. Probes comprising a crosslinking agent are combined with a sample which may comprise a target sequence which is complementary to the probe. Hybridization is allowed to occur between complementary sequences. The crosslinking agent is activated. Covalent bonds are formed between the probe and the target sequence if they are hybridized to one another. The crosslinked nucleic acids can then be detected to indicate the presence of the target sequence. Also provided are kits comprising reagents.

  提供了检测核酸序列的方法和组合物。含有交联剂的探针与样品结合，样品中可能含有与探针互补的目标序列。允许互补序列之间发生杂交。交联剂被激活。如果探针和目标序列相互杂交，它们之间就会形成共价键。然后可以检测交联的核酸，以显示目标序列的存在。此外，还提供由试剂组成的试剂盒。
• EP0796346A4
  申请人：——

  公开号：EP0796346A4

  公开（公告）日：2004-06-23
• NUCLEIC ACID SEQUENCE DETECTION EMPLOYING AMPLIFICATION PROBES
  申请人：NAXCOR

  公开号：EP0796346A1

  公开（公告）日：1997-09-24