(5Z)-5-[((3,5-二氟-4-羟基苯基)亚甲基]-3,5-二氢-2-甲基-3-(2,2,2-三氟乙基)-4H-咪唑-4-酮 | 1539318-36-9

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 中文名称: (5Z)-5-[((3,5-二氟-4-羟基苯基)亚甲基]-3,5-二氢-2-甲基-3-(2,2,2-三氟乙基)-4H-咪唑-4-酮
• 中文别名: (Z)-5-(3,5-二氟-4-羟基苯亚甲基)-2-甲基-3-(2,2,2-三氟乙基)-3,5-二氢-4H-咪唑-4-酮
• 英文名称: (Z)-4-(3,5-difluoro-4-hydroxybenzylidene)-2-methyl-1-(2,2,2-trifluoroethyl)-1H-imidazol-5(4H)-one
• 英文别名: Dfhbi 1T；(5Z)-5-[(3,5-difluoro-4-hydroxyphenyl)methylidene]-2-methyl-3-(2,2,2-trifluoroethyl)imidazol-4-one
• CAS: 1539318-36-9
• 化学式: C₁₃H₉F₅N₂O₂
• 分子量: 320.219
• InChiKey: AWYCLBWNRONMQC-WMZJFQQLSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  2.5
• 重原子数：
  22
• 可旋转键数：
  2
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.23
• 拓扑面积：
  52.9
• 氢给体数：
  1
• 氢受体数：
  8

反应信息

• 作为产物：
  描述：

  3,5-二氟-4-羟基苯甲醛 在 sodium acetate 、 potassium carbonate 作用下， 以 乙醇 为溶剂， 反应 5.0h， 生成 (5Z)-5-[((3,5-二氟-4-羟基苯基)亚甲基]-3,5-二氢-2-甲基-3-(2,2,2-三氟乙基)-4H-咪唑-4-酮
  参考文献：
  名称：

  Plug-and-Play Fluorophores Extend the Spectral Properties of Spinach

  摘要：

  Spinach and Spinach2 are RNA aptamers that can be used for the genetic encoding of fluorescent RNA. Spinach2 binds and activates the fluorescence of (Z)-4-(3,5-difluoro-4-hydroxybenzylidene)-1,2-dimethyl-1H-imidazol-5 (4H)-one (DFHBI), allowing the dynamic localizations of Spinach2-tagged RNAs to be imaged in live cells. The spectral properties of Spinach2 are limited by DFHBI, which produces fluorescence that is bluish-green and is not optimized for filters commonly used in fluorescence microscopes. Here we characterize the structural features that are required for fluorophore binding to Spinach2 and describe novel fluorophores that bind and are switched to a fluorescent state by Spinach2. These diverse Spinach2 fluorophore complexes exhibit fluorescence that is more compatible with existing microscopy filter sets and allows Spinach2-tagged constructs to be imaged with either GFP or YFP filter cubes. Thus, these "plug-and-play" fluorophores allow the spectral properties of Spinach2 to be altered on the basis of the specific spectral needs of the experiment.

  DOI：

  10.1021/ja410819x

文献信息

• FLUORESCENT APTAMERS AND THEIR APPLICATIONS
  申请人：Centre National de la Recherche Scientifique

  公开号：EP3211083A1

  公开（公告）日：2017-08-30

  The present invention relates to a nucleic acid molecule capable of binding to a fluorophore molecule, the nucleic acid molecule being a molecule containing a first and a second region, said first and second regions being such that : - the first region comprises the nucleotide sequence of SEQ ID NO: 1; and - the second region comprises the nucleotide sequence of SEQ ID NO: 2.

  本发明涉及一种能够与荧光团分子结合的核酸分子，该核酸分子是包含第一和第二区域的分子，所述第一和第二区域是：- 第一区域包含 SEQ ID NO: 1 的核苷酸序列；- 第二区域包含 SEQ ID NO: 2 的核苷酸序列。
• FLUOROGENIC NUCLEIC ACID MOLECULE AND TARGET-RNA FLUORESCENT LABELING METHOD
  申请人：Japan Science and Technology Agency

  公开号：EP3892725A1

  公开（公告）日：2021-10-13

  The present invention provides a fluorogenic RNA capable of visualizing mRNA in cells, particularly, living mammalian cells, and a target RNA fluorescent labeling method using the fluorogenic RNA. The present invention provides a fluorogenic nucleic acid molecule containing a base sequence having 2 or more fluorescent molecule-binding regions linked via a linker sequence, in which 1 or more fluorescent molecule-binding aptamer sequences are inserted into a scaffold sequence of each of the fluorescent molecule-binding regions.

  本发明提供了一种能够可视化细胞，特别是哺乳动物活细胞中mRNA的荧光RNA，以及一种使用该荧光RNA的靶RNA荧光标记方法。本发明提供了一种荧光核酸分子，该分子含有一个碱基序列，该碱基序列具有 2 个或 2 个以上通过连接序列连接的荧光分子结合区，其中 1 个或 1 个以上荧光分子结合适配体序列被插入到每个荧光分子结合区的支架序列中。
• NOVEL PROBE SET FOR ISOTHERMAL ONE-POT REACTION, AND USES THEREOF
  申请人：POSTECH Research and Business Development Foundation

  公开号：EP3943614A1

  公开（公告）日：2022-01-26

  The present invention relates to a novel probe set for an isothermal one-pot reaction, and uses thereof and, particularly, provides a method for easily, accurately, and quickly diagnosing molecules, in particular, infection diseases, in the field, the method having a form applicable in the field on the basis of a nucleic acid sequence by using a one-pot reaction composition under an isothermal condition. If a molecular diagnostic platform according to the present invention is used, a target sequence can be quickly and accurately detected. Also, elements (reaction buffer, enzyme) needed in a diagnostic process using the present invention are much more simple and convenient than in a conventional antibody-based diagnosis, and, thus, the diagnostic process can be performed by a non-skilled person, and the sensitivity and speed of diagnosis can be increased, as all reactions are unified at a constant temperature without expensive equipment used in general nucleic-acid-based molecular diagnostic technology, and an amplification process is performed automatically during a reaction process.

  本发明涉及一种用于等温一锅反应的新型探针组及其用途，特别是提供了一种在现场简便、准确、快速地诊断分子，尤其是感染疾病的方法，该方法通过使用等温条件下的一锅反应组合物，以核酸序列为基础，具有适用于现场的形式。如果使用本发明的分子诊断平台，就可以快速准确地检测目标序列。此外，使用本发明的诊断过程中所需的元素（反应缓冲液、酶）比传统的基于抗体的诊断简单方便得多，因此，诊断过程可由非专业人员完成，而且由于所有反应均在恒温条件下进行，无需使用一般基于核酸的分子诊断技术中使用的昂贵设备，而且在反应过程中自动执行扩增过程，因此可提高诊断的灵敏度和速度。
• Cascade/dCas3 complementation assays for in vivo detection of nucleic acid-guided nuclease edited cells
  申请人：Inscripta, Inc.

  公开号：US11008557B1

  公开（公告）日：2021-05-18

  The present disclosure relates to methods and compositions that allow one to identify in vivo edited cells when employing nucleic-acid guided editing. Additionally provided are automated multi-module instruments for performing editing and selection methods and using the compositions.

  本公开涉及在使用核酸引导编辑时可识别体内编辑细胞的方法和组合物。此外，还提供了用于执行编辑和选择方法以及使用组合物的自动化多模块仪器。
• Molecular fuses for real-time, label-free, multiplexed imaging of RNAs in living cells
  申请人：ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

  公开号：US11047000B2

  公开（公告）日：2021-06-29

  Provided herein are imaging probes and systems and methods employing such imaging probes for real-time, label-free, multiplexed imaging of RNAs in living cells. More particularly, aptamer-based sensors (“aptasensors”) and molecular fuses comprising multiple aptasensors are genetically encoded imaging probes comprising RNA-target binding sequence and an intramolecular reconfiguration sequence. The probe is configured such that binding of a RNA target by the RNA-target binding sequence triggers the intramolecular reconfiguration sequence to reconfigure such that an optically detectable output is generated by the probe.

  本文提供了成像探针以及使用这种成像探针对活细胞中的 RNA 进行实时、无标记、多路复用成像的系统和方法。更具体地说，基于适配体的传感器（"适配体传感器"）和由多个适配体传感器组成的分子融合体是基因编码的成像探针，包括 RNA 靶标结合序列和分子内重构序列。探针的配置方式是，RNA-靶标结合序列与 RNA 靶标结合，触发分子内重构序列进行重构，从而使探针产生可光学检测的输出。