我们描述了乙二胺四乙酸(EDTA)衍生物的合成途径,该衍生物可以通过立体化学独特方式连接到EDTA的C-1碳原子的甲基硫代磺酸盐部分连接到蛋白质中半胱氨酸残基的表面暴露的巯基官能团。此类化合物可用于在溶液中比对蛋白质,而无需添加液晶介质,因此对于生物分子的NMR光谱分析非常感兴趣。通过测量发光来确定顺磁性标签与镧系元素离子的结合常数。对于Tb(+3)-配体配合物,获得的K(b)值为6.5 x 10(17)M(-1)。该值与相关EDTA化合物的文献值非常一致。此外,
carboxylic acid and ester building blocks by diazotization of S-sulfonyl-cysteines. The thiosulfonate protecting group demonstrated resistance to oxidation and attenuation of sulfur's nucleophilicity by the anomeric effect. The key transformation was optimized by a 22 factorial design of experiment, highlighting the unique reactivity of cysteine derivatives in comparison with aliphatic amino acids.
We describe the synthetic route to ethylenediaminetetraacetic acid (EDTA) derivatives that can be attached to surface-exposed thiol functional groups of cysteine residues in proteins, via a methylthiosulfonate moiety that is connected in a stereochemically unique way to the C-1 carbon atom of EDTA. Such compounds can be used to align proteins in solution without the need to add liquid crystalline media
我们描述了乙二胺四乙酸(EDTA)衍生物的合成途径,该衍生物可以通过立体化学独特方式连接到EDTA的C-1碳原子的甲基硫代磺酸盐部分连接到蛋白质中半胱氨酸残基的表面暴露的巯基官能团。此类化合物可用于在溶液中比对蛋白质,而无需添加液晶介质,因此对于生物分子的NMR光谱分析非常感兴趣。通过测量发光来确定顺磁性标签与镧系元素离子的结合常数。对于Tb(+3)-配体配合物,获得的K(b)值为6.5 x 10(17)M(-1)。该值与相关EDTA化合物的文献值非常一致。此外,