1,3,6-芘三磺酸,8-甲氧基- | 121879-12-7

• 分子结构分类: 有机化合物 - 苯类化合物 - 芘
• 中文名称: 1,3,6-芘三磺酸,8-甲氧基-
• 英文名称: 8-methoxypyrene-1,3,6-trisulfonic Acid
• CAS: 121879-12-7
• 化学式: C₁₇H₁₂O₁₀S₃
• 分子量: 472.5
• InChiKey: CTERCLHSWSQHSD-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  1.2
• 重原子数：
  30
• 可旋转键数：
  4
• 环数：
  4.0
• sp3杂化的碳原子比例：
  0.06
• 拓扑面积：
  198
• 氢给体数：
  3
• 氢受体数：
  10

文献信息

• PROTEIN AFFINITY TAG AND USES THEREOF
  申请人：Zomer-Elhadari Jamila Willhelmina

  公开号：US20140017797A1

  公开（公告）日：2014-01-16

  This invention concerns isotopically coded or non-isotopically coded affinity-tags for analysis of certain target molecules in complex samples, in particular for mass spectrometric analysis of proteomic samples. The affinity-tags have the following general formula X-SPACER-OPO 3 H 2 , wherein X is a functional group or moiety capable of reacting with a functional group of a protein, peptide, DNA, lipid, sugar and/or steroid. These phosphate affinity tags (‘PTAG’) are capable of high but reversible binding to metal-oxides like TiO 2 . Due to this property, tagged sample fractions can be isolated from non-tagged sample fraction by affinity chromatography. The binding of organophosphate to metal-oxides remains intact during multiple washings of preferably acidic solutions to remove non-specifically bound components. PTAG's are also envisaged wherein X is selected such that it is capable of binding proteins, peptides, nucleic acid molecules, lipids, carbohydrates, steroids and the like.

  这项发明涉及同位素编码或非同位素编码的亲和标签，用于分析复杂样本中的特定靶分子，特别是用于蛋白质组样本的质谱分析。这些亲和标签具有以下一般公式X-SPACER-OPO3H2，其中X是能够与蛋白质、肽、DNA、脂质、糖和/或类固醇的功能基团反应的官能团或基团。这些磷酸亲和标签（“PTAG”）能够高效但可逆地与氧化金属如TiO2结合。由于这种特性，通过亲和色谱可以将标记样品分离出非标记样品分数。有机磷酸盐与金属氧化物的结合在多次使用酸性溶液洗涤以去除非特异性结合组分时仍然保持完好。还可以设想PTAG的情况，其中X被选择为能够结合蛋白质、肽、核酸分子、脂质、碳水化合物、类固醇等的官能团。
• Indirect colorimetric detection of an analyte in a sample using ratio of light signals
  申请人：Becton Dickinson and Company

  公开号：EP0278149A2

  公开（公告）日：1988-08-17

  The method of the invention employs an enzyme immunoassay for measuring the concentration of an analyte in a sample by indirect colorimetric detection. An incident light beam or a plurality of wavelengths is directed into a liquid solution containing an analyte of interest; the solution being capable of attentuating the amount of light at a first wavelength received from this solution as a function of the increasing concentration of the analyte present. A light signal from the solution at the first wavelength is detected, and light at a second wavelength, at which substantially no attenuation of light signal occurs as the concentration of the analyte increases, is also detected. The ratio of the two respective wavelengths is formed and that ratio is compared with ratios of known amounts of the analyte to determine the amount of tha analyte in the sample.

  本发明的方法采用酶免疫测定法，通过间接比色检测来测量样品中分析物的浓度。入射光束或多个波长的光束被射入含有相关分析物的液体溶液中；该溶液能够使从溶液中接收到的第一波长的光量随分析物浓度的增加而衰减。检测溶液中第一波长的光信号，同时检测第二波长的光信号，第二波长的光信号基本上不会随着分析物浓度的增加而衰减。形成两个各自波长的比率，并将该比率与已知量的分析物比率进行比较，以确定样品中的分析物含量。
• Slip chip device and methods
  申请人：University of Chicago

  公开号：US10543485B2

  公开（公告）日：2020-01-28

  A device is described having a first surface having a plurality of first areas and a second surface having a plurality of second areas. The first surface and the second surface are opposed to one another and can move relative to each other from at least a first position where none of the plurality of first areas, having a first substance, are exposed to plurality of second areas, having a second substance, to a second position. When in the second position, the plurality of first and second areas, and therefore the first and second substances, are exposed to one another. The device may further include a series of ducts in communication with a plurality of first second areas to allow for a substance to be disposed in, or upon, the plurality of second areas when in the first position.

  所述设备具有一个具有多个第一区域的第一表面和一个具有多个第二区域的第二表面。第一表面和第二表面彼此相对，至少可以从具有第一物质的多个第一区域都不接触具有第二物质的多个第二区域的第一位置相对移动到第二位置。当处于第二位置时，多个第一和第二区域以及第一和第二物质相互暴露。该装置可进一步包括一系列与多个第一和第二区域相通的管道，以便在处于第一位置时将物质置于多个第二区域内或其上。
• In situ detection of nucleotide variants in high noise samples, and compositions and methods related thereto
  申请人：Advanced Cell Diagnostics, Inc.

  公开号：US11078528B2

  公开（公告）日：2021-08-03

  The invention relates to methods of in situ detection of a nucleic acid variation of a target nucleic acid in a sample, including single nucleotide variations, multi-nucleotide variations or splice sites. The method can comprise the steps of contacting the sample with a probe that detects the nucleic acid variation or splice site and a neighbor probe; contacting the sample with pre-amplifiers that bind to the nucleic acid variation probe or splice site probe and neighbor probe, respectively; contacting the sample with a collaboration amplifier that binds to the pre-amplifiers; and contacting the sample with a label probe system, wherein hybridization of the components forms a signal generating complex (SGC) comprising a target nucleic acid with the nucleic acid variation or splice site, the probes and amplifiers; and detecting in situ signal from the SGC on the sample. The invention also provides samples, tissue slides, and kits relating to detection of nucleic acid variations, including single nucleotide variations, multi-nucleotide variations or splice sites, of a target nucleic acid.

  本发明涉及原位检测样品中目标核酸变异的方法，包括单核苷酸变异、多核苷酸变异或剪接位点。该方法可包括以下步骤：用检测核酸变异或剪接位点的探针和邻接探针接触样品；用分别与核酸变异探针或剪接位点探针和邻接探针结合的前置放大器接触样品；用与前置放大器结合的协作放大器接触样品；将样品与标记探针系统接触，其中各组分杂交形成信号发生复合物（SGC），该复合物由靶核酸与核酸变异或剪接位点、探针和放大器组成；检测样品上 SGC 的原位信号。本发明还提供了与检测核酸变异（包括目标核酸的单核苷酸变异、多核苷酸变异或剪接位点）有关的样品、组织切片和试剂盒。
• Chemical reagents and methods for detection and quantification of proteins in complex mixtures
  申请人：——

  公开号：US20040038319A1

  公开（公告）日：2004-02-26

  The invention provides a reagent comprising an affinity tag, a detectable moiety, a linker, an isotope tag and a reactive group. The invention also provides methods of using a reagent of the invention. The methods can be used to label a polypeptide in a sample by contacting a sample with a reagent of the invention under conditions allowing the reactive group to bind to one or more polypeptides in the sample. The invention additionally provides methods of isolating, identifying and quantifying a polypeptide in a sample. The invention further provides methods of diagnosing a disease using a reagent of the invention.

  本发明提供了一种试剂，该试剂由亲和性标签、可检测分子、连接体、同位素标签和反应基团组成。本发明还提供了使用本发明试剂的方法。在允许反应基团与样品中的一个或多个多肽结合的条件下，将样品与本发明的试剂接触，即可使用这些方法标记样品中的多肽。本发明还提供了分离、鉴定和量化样品中多肽的方法。本发明进一步提供了使用本发明试剂诊断疾病的方法。