The present invention relates generally to novel genetic sequences that encode fatty acid epoxygenase enzymes, in particular fatty acid &Dgr;12-epoxygenase enzymes from plants that are mixed function monooxygenase enzymes. More particularly, the present invention exemplifies cDNA sequences from Crepis spp. and
Vernonia galamensis
that encode fatty acid &Dgr;12-epoxygenases. The genetic sequences of the present invention provide the means by which fatty acid metabolism may be altered or manipulated in organisms, such as, for example, yeasts, moulds, bacteria, insects, birds, mammals and plants, and more particularly in plants. The invention also extends to genetically modified oil-accumulating organisms transformed with the subject genetic sequences and to the oils derived therefrom. The oils thus produced provide the means for the cost-effective raw materials for use in the efficient production of coatings, resins, glues, plastics, surfactants and lubricants.
本发明一般涉及编码
脂肪酸环氧酶的新型
基因序列,特别是来自植物的
脂肪酸&Dgr;12-环氧酶,它们是混合功能的单加氧酶。更具体地说,本发明举例说明了来自 Crepis spp.
Vernonia galamensis
编码
脂肪酸&Dgr;12-环氧合酶的 cDNA 序列。本发明的
基因序列提供了改变或操纵
生物体
脂肪酸代谢的方法,例如酵母、霉菌、细菌、昆虫、鸟类、哺乳动物和植物,尤其是植物。本发明还适用于用所述
基因序列转化的转
基因贮油
生物以及由此产生的油。由此产生的油类为高效生产涂料、
树脂、胶
水、塑料、表面活性剂和润滑剂提供了具有成本效益的原材料。