氯化新四唑 | 298-95-3

• 物质功能分类: 化学试剂 - 有机试剂 - 偶氮、重氮化合物
• 分子结构分类: 有机化合物 - 苯类化合物 - 苯和取代衍生物
• 中文名称: 氯化新四唑
• 英文名称: Neotetrazolium chloride
• 英文别名: 2-[4-[4-(3,5-diphenyltetrazol-2-ium-2-yl)phenyl]phenyl]-3,5-diphenyltetrazol-2-ium;dichloride
• CAS: 298-95-3
• 化学式: C38H28Cl2N8
• 分子量: 667.6
• InChiKey: WYFYSTBFFDOVJW-UHFFFAOYSA-L

物化性质

• 熔点：
  230 °C (dec.)(lit.)
• 沸点：
  754.15°C (rough estimate)
• 密度：
  1.0962 (rough estimate)
• 溶解度：
  溶于甲醇，溶解度为50mg/mL，澄清
• 最大波长(λmax)：
  248 nm

计算性质

• 辛醇/水分配系数(LogP)：
  0.41
• 重原子数：
  48
• 可旋转键数：
  7
• 环数：
  8.0
• sp3杂化的碳原子比例：
  0.0
• 拓扑面积：
  69.2
• 氢给体数：
  0
• 氢受体数：
  6

安全信息

• 危险品标志：
  Xn
• 安全说明：
  S24/25,S36
• 危险类别码：
  R22
• WGK Germany：
  3

反应信息

• 作为反应物：
  描述：

  氯化新四唑 、 异烟肼 以0%的产率得到
  参考文献：
  名称：

  Issopoulos P. B., Economou P. T., Analusis, 20 (1992) N 1, S 31-35

  摘要：

  DOI：

文献信息

• Reagents and methods for direct labeling of nucleotides
  申请人：Naleway John J.

  公开号：US20130150254A1

  公开（公告）日：2013-06-13

  The present invention provides systems and methods for production of activatable diazo-derivatives for use in labeling nucleotides. Labeling nucleotides is accomplished by contacting a stable hydrazide derivative of a detectable moiety with an activating polymer reagent which is used to directly label the nucleotide sample. Labeling occurs on the phosphate backbone of the nucleotide which does not perturb hybridization of the labeled nucleotide with its anti-sense strand. Since the method involves direct labeling, all types of nucleotides can be labeled without prior amplification or alteration.

  本发明提供了用于生产可激活重氮衍生物以用于标记核苷酸的系统和方法。通过将可检测基团的稳定的肼酰肼衍生物与用于直接标记核苷酸样品的活化聚合物试剂接触来完成核苷酸的标记。标记发生在核苷酸的磷酸骨架上，不会干扰标记核苷酸与其反义链的杂交。由于该方法涉及直接标记，所有类型的核苷酸都可以在不经过扩增或改变的情况下进行标记。
• REAGENTS AND METHODS FOR DIRECT LABELING OF NUCLEOTIDES
  申请人：MARKER GENE TECHNOLOGIES, INC.

  公开号：US20150152476A1

  公开（公告）日：2015-06-04

  The present invention provides systems and methods for production of activatable diazo-derivatives for use in labeling nucleotides. Labeling nucleotides is accomplished by contacting a stable hydrazide derivative of a detectable moiety with an activating polymer reagent which is used to directly label the nucleotide sample. Labeling occurs on the phosphate backbone of the nucleotide which does not perturb hybridization of the labeled nucleotide with its anti-sense strand. Since the method involves direct labeling, all types of nucleotides can be labeled without prior amplification or alteration.

  本发明提供了用于生产可激活的重氮衍生物以用于标记核苷酸的系统和方法。标记核苷酸是通过将可检测基团的稳定肼基衍生物与用于直接标记核苷酸样品的活化聚合物试剂接触来完成的。标记发生在核苷酸的磷酸骨架上，不会干扰标记核苷酸与其反义链的杂交。由于该方法涉及直接标记，因此所有类型的核苷酸都可以在不进行扩增或改变的情况下进行标记。
• Method of discrimination of microorganisms, composition and element for the implantation thereof
  申请人：EASTMAN KODAK COMPANY (a New Jersey corporation)

  公开号：EP0107594A1

  公开（公告）日：1984-05-02

  Disclosed herein is a method, element and compostion for substantially preventing the reduction of a compound in admixture with a gram-positive microorganism capable of reducing said compound. The method comprises including in said admixture an anionic surfactant in an amount sufficient to inhibit the reduction capability of the gram-positive microorganisms. The method provides a means for rapidly discriminating between gram-positive and gram-negative microorganisms. Elements and compositions for implementing the method are also disclosed.

  本发明公开了一种方法、元件和混合物，用于基本防止与能够还原所述化合物的革兰氏阳性微生物混合的化合物被还原。该方法包括在所述混合物中加入一种阴离子表面活性剂，其用量足以抑制革兰氏阳性微生物的还原能力。该方法提供了一种快速区分革兰氏阳性微生物和革兰氏阴性微生物的方法。还公开了实施该方法的元件和组合物。
• Inhibition of reduction activities of leukocytes
  申请人：EASTMAN KODAK COMPANY (a New Jersey corporation)

  公开号：EP0113103A1

  公开（公告）日：1984-07-11

  A method for detecting bacteria in an aqueous sample containing leukocytes comprising a) contacting said sample with a reducible indicator which forms a detectable end product b) incubating the sample of step a) and c) detecting the amount of bacteria present as a function of end product formed by reduction in step b) characterized in that during or prior to step a) the leukocytes in the sample are removed by selective lysis.

  一种检测含有白细胞的水样中细菌的方法，包括 a) 将所述样品与可还原指示剂接触，形成可检测的最终产物；b) 培养步骤 a) 中的样品；c) 检测存在的细菌数量与步骤 b) 中还原形成的最终产物的函数关系，其特征在于，在步骤 a) 期间或之前，通过选择性裂解去除样品中的白细胞。
• Isolation and quantitation of alkaline phosphatase
  申请人：AKZO N.V.

  公开号：EP0166505A2

  公开（公告）日：1986-01-02

  There is disclosed a process for isolating and quantitating an alkaline phosphatase comprising the steps of: (1) separating the phosphatase from other enzymes in a biological sample; (2) reacting the phosphatase from step (1) with a co-enzyme precursor to produce a co-enzyme; (3) employing the co-enzyme produced in step (2) in a series of chemical reactions at least one of which uses a chromophore generation catalyst, to yield a chromophore, and (4) measuring the absorbance of the chromophore which is generated in step (3).

  本发明公开了一种分离和定量检测碱性磷酸酶的方法，包括以下步骤 (1) 将生物样品中的磷酸酶与其他酶分离； (2) 将步骤(1)中的磷酸酶与辅酶前体反应生成辅酶； (3) 将步骤(2)中生成的辅酶用于一系列化学反应，其中至少有一个反应使用发色团生成催化剂，以生成发色团，以及 (4) 测量步骤(3)中生成的发色团的吸光度。

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