2,3-二羟基-2-甲基戊酸 | 597-02-4

• 分子结构分类: 有机化合物 - 脂质和类脂质分子 - 脂肪酰基
• 中文名称: 2,3-二羟基-2-甲基戊酸
• 英文名称: 2,3-dihydroxy-2-methyl-valeric acid
• 英文别名: α.β-Dioxy-α-methyl-n-valeriansaeure；β.γ-Dioxy-pentan-β-carbonsaeure；2,3-Dihydroxy-2-methyl-valeriansaeure；2-Methyl-pentandiol-(2.3)-saeure-(1)；2,3-Dihydroxy-2-methylpentanoic acid
• CAS: 597-02-4
• 化学式: C₆H₁₂O₄
• mdl: MFCD19229436
• 分子量: 148.159
• InChiKey: WBPIKRWVCLNWOS-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -0.4
• 重原子数：
  10
• 可旋转键数：
  3
• 环数：
  0.0
• sp3杂化的碳原子比例：
  0.833
• 拓扑面积：
  77.8
• 氢给体数：
  3
• 氢受体数：
  4

反应信息

• 作为产物：
  描述：

  2-甲基-2-戊烯醛 在 氧气 作用下， 生成 2,3-二羟基-2-甲基戊酸
  参考文献：
  名称：

  Lieben; Zeisel, Monatshefte fur Chemie, 1883, vol. 4, p. 52

  摘要：

  DOI：

文献信息

• Identification and use of Bacterial [2Fe-2S] dihydroxy-acid dehydratases
  申请人：Butamax Advanced Biofuels LLC

  公开号：EP2821484A1

  公开（公告）日：2015-01-07

  A group of bacterial dihydroxy-acid dehydratases having a [2Fe-2S] cluster was discovered. Bacterial [2Fe-2S] DHADs were expressed as heterologous proteins in bacteria and yeast cells, providing DHAD activity for conversion of 2,3-dihydroxyisovalerate to α-ketoisovalerate or 2,3-dihydroxymethylvalerate to α-ketomethylvalerate. Isobutanol and other compounds may be synthesized in pathways that include bacterial [2Fe-2S] DHAD activity.

  发现了一组具有 [2Fe-2S] 簇的细菌二羟基酸脱水酶。细菌[2Fe-2S] DHADs 在细菌和酵母细胞中以异源蛋白的形式表达，具有将 2,3-二羟基异戊酸转化为 α-酮异戊酸或将 2,3-二羟甲基戊酸转化为 α-酮甲基戊酸的 DHAD 活性。异丁醇和其他化合物可能通过包括细菌 [2Fe-2S] DHAD 活性在内的途径合成。
• DHAD variants and methods of screening
  申请人：Butamax Advanced Biofuels LLC

  公开号：US10287566B2

  公开（公告）日：2019-05-14

  Methods of screening for dihydroxy-acid dehydratase (DHAD) variants that display increased DHAD activity are disclosed, along with DHAD variants identified by these methods. Such enzymes can result in increased production of compounds from DHAD requiring biosynthetic pathways. Also disclosed are isolated nucleic acids encoding the DHAD variants, recombinant host cells comprising the isolated nucleic acid molecules, and methods of producing butanol.

  本研究公开了筛选二羟基酸脱水酶（DHAD）变体的方法，以及通过这些方法鉴定出的二羟基酸脱水酶变体。这种酶可导致需要 DHAD 生物合成途径的化合物产量增加。还公开了编码 DHAD 变异体的分离核酸、包含分离核酸分子的重组宿主细胞以及生产丁醇的方法。
• Activity of Fe—S cluster requiring proteins
  申请人：Butamax Advanced Biofuels LLC

  公开号：US10308964B2

  公开（公告）日：2019-06-04

  The present invention is related to a recombinant host cell, in particular a yeast cell, comprising a dihydroxy-acid dehydratase polypeptide. The invention is also related to a recombinant host cell having increased specific activity of the dihydroxy-acid dehydratase polypeptide as a result of increased expression of the polypeptide, modulation of the Fe—S cluster biosynthesis of the cell, or a combination thereof. The present invention also includes methods of using the host cells, as well as, methods for identifying polypeptides that increase the flux in an Fe—S cluster biosynthesis pathway in a host cell.

  本发明涉及一种含有二羟基酸脱水酶多肽的重组宿主细胞，特别是酵母细胞。本发明还涉及一种重组宿主细胞，由于该多肽表达量的增加、细胞中Fe-S簇生物合成的调节或它们的组合，该细胞中二羟基酸脱水酶多肽的特异性活性增加。本发明还包括使用宿主细胞的方法，以及鉴定增加宿主细胞中Fe-S簇生物合成途径通量的多肽的方法。
• Lieben; Zeisel, Monatshefte fur Chemie, 1883, vol. 4, p. 77
  作者：Lieben、Zeisel

  DOI：——

  日期：——
• FUNGAL SIGNALLING AND METABOLIC ENZYMES
  申请人：F2G Ltd.

  公开号：EP1825272A2

  公开（公告）日：2007-08-29