The invention provides novel methods for the separation of charged molecules such as proteins according to the isoelectric points (pI's) and includes the systems and buffering compositions employed for isolating charged molecules. The invention further provides for modifications to the above described chromatographic methods that enable the separation of charged molecules exhibiting virtually identical pI's by shifting both the buffer's pKa and the pI's of the eluted charged molecules while they are traversing the ion exchange column.
US4334972A
申请人:——
公开号:US4334972A
公开(公告)日:1982-06-15
US7790025B2
申请人:——
公开号:US7790025B2
公开(公告)日:2010-09-07
[EN] EXTERNAL GRADIENT CHROMATOFOCUSING<br/>[FR] CHROMATOFOCALISATION A GRADIENT EXTERNE
申请人:DNAVAX INC
公开号:WO2004103519A2
公开(公告)日:2004-12-02
The invention provides novel methods for the separation of charged molecules such a proteins according to their isoelectric points (pI's) and includes the systems and buffering compositions employed for isolating charged molecules. The invention further provides for modifications to the above described chromatographic methods that enable the separation of charged molecules exhibiting virtually identical pl's by shifting both the buffer's pKa and the pl's of the eluted charged molecules while they are traversing the ion exchange column.