生物素XX酰肼 | 211237-33-1

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 生物素及其衍生物
• 中文名称: 生物素XX酰肼
• 英文名称: N-(6-hydrazinyl-6-oxohexyl)-6-(5-(2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanamide
• 英文别名: N-(6-Hydrazinyl-6-oxohexyl)-6-{[5-(2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoyl]amino}hexanamide；N-(6-hydrazinyl-6-oxohexyl)-6-[5-(2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl)pentanoylamino]hexanamide
• CAS: 211237-33-1
• 化学式: C₂₂H₄₀N₆O₄S
• 分子量: 484.663
• InChiKey: LOIYZIMLLRKKNS-UHFFFAOYSA-N

物化性质

• 溶解度：
  DMSO：5mg/mL； DMSO:PBS(pH7.2) (1:1): 0.5 mg/mL

计算性质

• 辛醇/水分配系数(LogP)：
  0
• 重原子数：
  33
• 可旋转键数：
  17
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.82
• 拓扑面积：
  180
• 氢给体数：
  6
• 氢受体数：
  6

制备方法与用途

生物活性

Biotin-XX hydrazide（即生物素-(AC5)2-羟基胺）是一种反应性生物素化试剂，含有两个氨基己酸间隔基。这种化合物具有更高的抗生物素蛋白结合效率。

体外研究

Biotin-XX hydrazide 包含两个氨基己酸间隔基，这显著提高了生物素标记抗体（或其他生物素标记蛋白质）与 avidin 探针共轭物之间复合物形成的效率。此处的探针可以是荧光染料或酶。

反应信息

• 作为反应物：
  描述：

  2-[[(4-氨基苯基)磺酰基]氨基]-4-噻唑乙酸 、 生物素XX酰肼 在 N,N'-羰基二咪唑 作用下， 以 N,N-二甲基甲酰胺 为溶剂， 反应 2.0h， 生成 6-[5-((3aR,6S,6aS)-2-Oxo-hexahydro-thieno[3,4-d]imidazol-6-yl)-pentanoylamino]-hexanoic acid [6-(N'-{2-[2-(4-amino-benzenesulfonylamino)-thiazol-4-yl]-acetyl}-hydrazino)-6-oxo-hexyl]-amide
  参考文献：
  名称：

  Improving Broad Specificity Hapten Recognition with Protein Engineering

  摘要：

  Sulfa antibiotics (sulfonamides) are derivatives of p-aminobenzenesulfonamide that are widely used in veterinary medicine. Foods derived from treated animals may be contaminated with these drugs. However, current immunobased sulfonamide detection methods are unfit for screening of products because they are either too insensitive or specific for a few compounds only. An immunoassay capable of detecting all sulfas in a single reaction would be ideal for screening. For development of a binder capable of binding all sulfas, a protein engineering approach was chosen and the properties of monoclonal antibody 27G3 were improved with mutagenesis followed by selection with phage display. Several different mutant antibodies were isolated. The cross-reaction profile of the best mutant antibody was significantly improved over that of the wild-type antibody: it was capable of binding 9 of the tested 13 sulfonamides within a narrow concentration range and also bound the rest of the sulfas, albeit within a wider concentration range.

  DOI：

  10.1021/jf0200624

文献信息

• [EN] HALOFUGINONE ANALOGS FOR INHIBITION OF TRNA SYNTHETASES AND USES THEREOF<br/>[FR] ANALOGUES D'HALOFUGINONE POUR L'INHIBITION D'ARNT SYNTHÉTASES ET LEURS UTILISATIONS
  申请人：HARVARD COLLEGE

  公开号：WO2010019210A3

  公开（公告）日：2010-05-14
• HALOFUGINONE ANALOGS FOR INHIBITION OF TRNA SYNTHETASES AND USES THEREOF
  申请人：Keller Tracy

  公开号：US20110263532A1

  公开（公告）日：2011-10-27

  The present invention provides novel analogs and derivatives of halofuginone. The invention also provides pharmaceutical and cosmetic compositions thereof and methods for using halofuginone analogs in treating chronic inflammatory diseases, autoimmune diseases, dry eye syndrome, fibrosis, scar formation, angiogenesis, viral infections, ischemic damage, transplant and implant rejection, neurodegenerative diseases, and cosmetic applications.
• US9284297B2
  申请人：——

  公开号：US9284297B2

  公开（公告）日：2016-03-15
• Improving Broad Specificity Hapten Recognition with Protein Engineering
  作者：Teemu Korpimäki、Jaana Rosenberg、Pekka Virtanen、Tuomas Karskela、Urpo Lamminmäki、Mika Tuomola、Markus Vehniäinen、Petri Saviranta

  DOI：10.1021/jf0200624

  日期：2002.7.1

  Sulfa antibiotics (sulfonamides) are derivatives of p-aminobenzenesulfonamide that are widely used in veterinary medicine. Foods derived from treated animals may be contaminated with these drugs. However, current immunobased sulfonamide detection methods are unfit for screening of products because they are either too insensitive or specific for a few compounds only. An immunoassay capable of detecting all sulfas in a single reaction would be ideal for screening. For development of a binder capable of binding all sulfas, a protein engineering approach was chosen and the properties of monoclonal antibody 27G3 were improved with mutagenesis followed by selection with phage display. Several different mutant antibodies were isolated. The cross-reaction profile of the best mutant antibody was significantly improved over that of the wild-type antibody: it was capable of binding 9 of the tested 13 sulfonamides within a narrow concentration range and also bound the rest of the sulfas, albeit within a wider concentration range.