3'-deoxy-2-thiothymidine | 162894-47-5

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘧啶核苷
• 英文名称: 3'-deoxy-2-thiothymidine
• 英文别名: 1-[(2R,5S)-5-(hydroxymethyl)tetrahydrofuran-2-yl]-5-methyl-2-thioxo-pyrimidin-4-one；1-[(2R,5S)-5-(hydroxymethyl)oxolan-2-yl]-5-methyl-2-sulfanylidenepyrimidin-4-one
• CAS: 162894-47-5
• 化学式: C₁₀H₁₄N₂O₃S
• 分子量: 242.299
• InChiKey: WTJGNHUYUJHHID-JGVFFNPUSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -1.1
• 重原子数：
  16
• 可旋转键数：
  2
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.6
• 拓扑面积：
  93.9
• 氢给体数：
  2
• 氢受体数：
  4

反应信息

• 作为反应物：
  描述：

  3'-deoxy-2-thiothymidine 在 吡啶 、 甲醇 、 氨 、 三乙胺 、 三氯氧磷 作用下， 以 乙腈 为溶剂， 反应 24.0h， 生成 2',3'-dideoxy-5-methyl-2-thiocytidine
  参考文献：
  名称：

  Synthesis of Pyrimidine 2′,3′-Dideoxy-2-Thionucleosides

  摘要：

  2'-Deoxyuridine 8a and thymidine 8b were converted in eight steps and in satisfactory overall yields into 2',3'-dideoxy-2-thiouridine (ddTU) 6a and 3'-deoxy-2-thiothymidine (ddTT) 6b, respectively. A three-step procedure is described for the conversion of ddTU 6a and ddTT 6b into the corresponding 2',3'-dideoxycytidine derivatives (ddTC 7a and ddMTC 7b, respectively) in good overall yield.

  DOI：

  10.1080/15257779508014664
• 作为产物：
  描述：

  5'-O-tritylthymidine 在 吡咯 、 偶氮二异丁腈 、 硫化氢 、 三正丁基氢锡 、 三乙胺 、 三氟乙酸 、 四甲基胍 作用下， 以 吡啶 、 乙醇 、 二氯甲烷 、 苯 为溶剂， 反应 10.25h， 生成 3'-deoxy-2-thiothymidine
  参考文献：
  名称：

  Synthesis of Pyrimidine 2′,3′-Dideoxy-2-Thionucleosides

  摘要：

  2'-Deoxyuridine 8a and thymidine 8b were converted in eight steps and in satisfactory overall yields into 2',3'-dideoxy-2-thiouridine (ddTU) 6a and 3'-deoxy-2-thiothymidine (ddTT) 6b, respectively. A three-step procedure is described for the conversion of ddTU 6a and ddTT 6b into the corresponding 2',3'-dideoxycytidine derivatives (ddTC 7a and ddMTC 7b, respectively) in good overall yield.

  DOI：

  10.1080/15257779508014664

文献信息

• Population of nucleic acids including a subpopulation of lna oligomers
  申请人：Ramsing Birger Neils

  公开号：US20060147924A1

  公开（公告）日：2006-07-06

  The present invention relates to a population of nucleic acids, said population comprising a first population of nucleic acids of the same length, said length being in the range of 5-15 nucleotides or units, said first population representing at least 1% of the possible different nucleic acid sequences for nucleic acids of said length, at least one nucleic acid in the first population being an LNA (Locked Nucleoside Analogues) oligomer. The population of oligonucleotides is preferably bonded to a solid support. The pluralities of nucleic acids are particularly useful in methods relating to the capture of target nucleic acids, or as probes, e.g. PCR probes. The invention also features LNA oligomers wherein the LNA units have SBC (Selective Binding Complementary) nucleobases.

  本发明涉及一种核酸群体，该群体包括一种长度相同的第一种核酸群体，该长度在5-15个核苷酸或单位的范围内，该第一种群体代表该长度的不同核酸序列中至少1％，第一种群体中至少有一个核酸是LNA（锁定核苷酸类似物）寡聚体。寡核苷酸的群体最好与固定支撑物结合。这些核酸的多样性在与目标核酸的捕获相关的方法或作为探针，例如PCR探针的方法中特别有用。本发明还涉及具有SBC（选择性结合互补）核碱基的LNA寡聚体。
• OLIGONUCLEOTIDES USEFUL FOR DETECTING AND ANALYZING NUCLEIC ACIDS OF INTEREST
  申请人：Kauppinen Sakari

  公开号：US20120157333A1

  公开（公告）日：2012-06-21

  The invention features improved nucleic acids and methods for expression profiling of mRNAs, identifying and profiling of particular mRNA splice variants, and detecting mutations, deletions, or duplications of particular exons or other splice variants, e.g., alterations associated with a disease such as cancer, in a nucleic acid sample, e.g., a biological sample or a patient sample.

  这项发明涉及改进的核酸和表达谱分析mRNA的方法，识别和分析特定的mRNA剪接变体，以及检测特定外显子或其他剪接变体的突变、缺失或复制，例如与疾病如癌症相关的变化，可以在核酸样品中进行检测，例如生物样品或患者样品。
• A population of nucleic acids including a subpopulation of LNA oligomers
  申请人：Exiqon A/S

  公开号：EP1882748A2

  公开（公告）日：2008-01-30

  The present application discloses LNA-2,6-diaminopurine (LNA-D) and LNA-2-aminopurine (LNA-2AP) of the formulae and corresponding methods for the preparation thereof, as well as LNA oligomers comprising such LNA-2,6-diaminopurine (LNA-D) and/or LNA-2-aminopurine (LNA-2AP) units. The application also discloses a pair of substantially complementary oligonucleotides, each comprising, in pairwise opposing positions, one or more SBC nucleotides or units, wherein at least one of the oligonucleotides is an LNA oligomer having SBC LNA units.

  本申请公开了式中的 LNA-2,6-二氨基嘌呤（LNA-D）和 LNA-2-aminopurine （LNA-2AP）。 及其相应的制备方法，以及包含此类 LNA-2,6-二氨基嘌呤 (LNA-D) 和/或 LNA-2-aminopurine (LNA-2AP) 单元的 LNA 低聚物。该申请还公开了一对基本互补的寡核苷酸，每个寡核苷酸在成对相对的位置上包含一个或多个 SBC 核苷酸或单元，其中至少一个寡核苷酸是具有 SBC LNA 单元的 LNA 寡聚体。
• Cell-free protein expression using rolling circle amplification product
  申请人：General Electric Company

  公开号：US10077459B2

  公开（公告）日：2018-09-18

  Methods for in vitro transcription and translation from an RCA product are provided. The methods comprise providing a double-stranded RCA product, wherein the double-stranded RCA product consists essentially of tandem repeats of a minimalistic expression sequence. The methods further comprise expressing a protein from the double-stranded RCA product in a cell-free expression system.

  提供了从 RCA 产物进行体外转录和翻译的方法。这些方法包括提供双链 RCA 产物，其中双链 RCA 产物基本上由最小表达序列的串联重复组成。这些方法还包括在无细胞表达系统中表达来自双链 RCA 产物的蛋白质。
• In vivo RNA or protein expression using double-stranded concatemeric DNA including phosphorothioated nucleotides
  申请人：General Electric Company

  公开号：US10350307B2

  公开（公告）日：2019-07-16

  A method for in vivo RNA or protein expression is provided. The method includes introducing a double-stranded concatemeric DNA into a eukaryotic cell to generate a desired RNA or protein. The double-stranded concatemeric DNA includes a plurality of tandem repeat sequences, wherein each of the plurality of tandem repeat sequences comprises an expression sequence. The double-stranded concatemeric DNA comprises one or more phosphorothioated nucleotides, wherein a ratio of phosphorothioated nucleotides to total nucleotides in the double-stranded concatemeric DNA is at least 1:1600. A eukaryotic cell comprising an exogeneous, double-stranded concatemeric DNA comprising the plurality of tandem repeat sequences is also provided.

  提供了一种体内 RNA 或蛋白质表达的方法。该方法包括将双链聚合 DNA 导入真核细胞以产生所需的 RNA 或蛋白质。双链聚合 DNA 包括多个串联重复序列，其中多个串联重复序列中的每一个都包括表达序列。双链聚合 DNA 包括一个或多个硫代磷酸核苷酸，其中双链聚合 DNA 中硫代磷酸核苷酸与总核苷酸的比例至少为 1:1600。还提供了一种真核细胞，该细胞包含外源双链聚合 DNA，其中包含多个串联重复序列。