[EN] METHOD OF PREPARATION OF A BIOSENSING SURFACE AND BIOSENSING SURFACE<br/>[FR] PROCEDE DE PREPARATION D'UNE SURFACE DE BIODETECTION ET SURFACE DE BIODETECTION
申请人:PELTONEN JOUKO
公开号:WO2003052423A1
公开(公告)日:2003-06-26
The present invention relates to a method of preparation of a biosensing surface where a host matrix comprising at least one analyte specific binding agent and at least one analytically inert matrix agent comprising binding units is dissolved to form a host matrix solution. The host matrix solution is applied onto a subphase surface, where it forms an oriented monolayer, which is compressed. A solid metal substrate is brought in contact with the monolayer on the subphase surface, and the monolayer is transferred onto the solid substrate by using hydrophobic interactions between the monolayer and the solid substrate. The monolayer is also bound covalently to the solid substrate via binding units comprised in the analytically inert matrix agent molecules of the host matrix. The invention relates also to a biosensing surface.