An isolated nucleic acid fragment encoding a cytochrome P450 enzyme associated with the synthesis of plant &Dgr;
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-epoxy fatty acids is disclosed. The invention also relates to making a chimeric construct encoding all or a portion of the cytochrome P450 enzymes associated with the synthesis of plant &Dgr;
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-epoxy fatty acids, in sense or antisense orientation, wherein expression of the chimeric construct results in production of altered levels of the cytochrome P450 enzymes associated with the synthesis of plant &Dgr;
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-epoxy fatty acids in a transformed host cell.
一种分离的核酸片段,编码与植物 &Dgr 合成有关的细胞色素 P450 酶;
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-环氧
脂肪酸相关的细胞色素 P450 酶的分离核酸片段。本发明还涉及一种嵌合构建体,该构建体编码与植物&Dgr; 12 -环氧
脂肪酸合成相关的全部或部分细胞色素 P450 酶;
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-环氧
脂肪酸的全部或部分编码的有义或反义方向的嵌合构建体,其中表达该嵌合构建体导致产生与植物 &Dgr; 12 - 环氧
脂肪酸合成相关的细胞色素 P450 酶的
水平改变;
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-环氧
脂肪酸的细胞色素 P450 酶
水平的改变。