| 1293955-24-4

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘧啶核苷酸
• CAS: 1293955-24-4
• 化学式: C₇₇H₈₁Cl₂N₁₄O₂₁P₃
• 分子量: 1702.4
• InChiKey: VJTUGDISWZMIGW-COXLESGVSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  13.64
• 重原子数：
  117.0
• 可旋转键数：
  33.0
• 环数：
  14.0
• sp3杂化的碳原子比例：
  0.38
• 拓扑面积：
  418.46
• 氢给体数：
  4.0
• 氢受体数：
  31.0

反应信息

• 作为产物：
  描述：

  、 2-氰乙基N,N-二异丙基氯亚磷酰胺 在 N,N-二异丙基乙胺 作用下， 以 四氢呋喃 为溶剂， 反应 1.5h， 以41.2%的产率得到
  参考文献：
  名称：

  Synthetic Approach to Stop-Codon Scanning Mutagenesis

  摘要：

  A general combinatorial mutagenesis strategy using common dimethoxytrityl-protected mononucleotide phosphoramidites and a single orthogonally protected trinucleotide phosphoramidite (Fmoc-TAG; Fmoc = 9-fluorenylmethoxycarbonyl) was developed to scan a gene with the TAG amber stop codon with complete synthetic control. In combination with stop-codon suppressors that insert natural (e.g., alanine) or unnatural (e.g., p-benzoylphenylalanine, Bpa) amino acids, a single DNA library can be used to incorporate different amino acids for diverse purposes. Here, we scanned TAG codons through part of the gene for a model four-helix bundle protein, Rop, which regulates the copy number of ColE1 plasmids. Alanine was incorporated into Rop for mapping its binding site using an in vivo activity screen, and subtle but important differences from in vitro gel-shift studies of Rop function are evident. As a test, Bpa was incorporated using a Phe14 amber mutant isolated from the scanning library. Surprisingly, Phel4Bpa-Rop is weakly active, despite the critical role of Phe14 in Rop activity. Bpa is a photoaffinity label unnatural amino acid that can form covalent bonds with adjacent molecules upon UV irradiation. Irradiation of Phe14Bpa-Rop, which is a dimer in solution like wildtype Rop, results in covalent dimers, trimers, and tetramers. This suggests that Phel4Bpa-Rop weakly associates as a tetramer in solution and highlights the use of Bpa cross-linking as a means of trapping weak and transient interactions.

  DOI：

  10.1021/ja106894g