An object of the present invention is to provide a highly versatile, simple and safe method for measuring Lp-PLA2 activity. Another object of the present invention is to provide an accurate and highly sensitive method for measuring Lp-PLA2 activity. Provided is a method for measuring lipoprotein-associated phospholipase A2 (Lp-PLA2) activity in a sample containing Lp-PLA2, the method comprising the following steps (A) to (C):
(A) converting PAFs into lyso-PAFs by reacting the PAFs with the Lp-PLA2 in the sample;
(B) hydrolyzing the lyso-PAFs produced in the step (A) with an enzyme (lyso-PAF-PLD) to obtain hydrolysate; and
(C) measuring Lp-PLA2 activity in the sample by utilizing a quantitative change attributable to the hydrolysate obtained in step (B) as an indicator.
本发明的一个目的是提供一种高度通用、简单和安全的 Lp-P
LA2 活性测量方法。本发明的另一个目的是提供一种准确和高灵敏度的 Lp-P
LA2 活性测量方法。本发明提供了一种测量含 Lp-P
LA2 样品中脂蛋白相关
磷脂酶 A2 (Lp-P
LA2) 活性的方法,该方法包括以下步骤 (A) 至 (C):
(A) 通过使 PAFs 与样品中的 Lp-P
LA2 反应,将 PAFs 转化为溶菌
磷脂酶 A2;
(B) 用一种酶(溶菌酶-PAF-PLD)
水解步骤(A)中产生的溶菌酶-PAFs,以获得
水解物;及
(C) 利用可归因于步骤(B)中获得的
水解产物的定量变化作为指标,测量样品中的 Lp-P
LA2 活性。